Human/Mouse EphB6 Antibody

Catalog # Availability Size / Price Qty
MAB3384
MAB3384-SP
Product Details
FAQs
Supplemental Products
Reviews (1)

Human/Mouse EphB6 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse EphB6 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) EphB6 is observed, 25% cross-reactivity with recombinant human (rh) EphA10 is observed, and no cross-reactivity with rhEphA1, 2, 5, rhEphB3, 4, rmEphA1, 3, 4, 6, 7, 8, rmEphB2, 3, recombinant rat (rr) EphA5, or rrEphB1 is observed.
Source
Monoclonal Mouse IgG2B Clone # 465323
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human EphB6
Val17-Thr579
Accession # O15197
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human EphB6 Fc Chimera (Catalog # 3384-B6)
Recombinant Mouse EphB6 Fc Chimera (Catalog # 611-B6)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphB6

EphB6, also known as Hep and Mep, is a 110 kDa type I transmembrane glycoprotein in the Eph receptor tyrosine kinase family. EphB6 is expressed in brain, pancreas, thymus, kidney, and on peripheral T cells. Depending on concentration, Ephrin-B2 can either inhibit or promote EphB6 mediated cell adhesion and migration. EphB6 participates in T cell activation, and its level of expression is inversely correlated with tumor aggressiveness in a variety of malignancies. Within the ECD, human EphB6 shares 91% aa sequence identity with mouse and rat EphB6.

Long Name
Eph Receptor B6
Entrez Gene IDs
2051 (Human); 13848 (Mouse)
Alternate Names
EC 2.7.10.1; EPH receptor B6; EphB6; ephrin type-B receptor 6; Hep; HEPTyrosine-protein kinase-defective receptor EPH-6; Mep; MGC129910; MGC129911

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse EphB6 Antibody

Average Rating: 5 (Based on 1 Review)

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Human/Mouse EphB6 Antibody
By Anonymous on 12/07/2020
Application: Microarray Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma