Human/Mouse EphB6 Antibody

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Human/Mouse EphB6 Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse EphB6 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) EphB6 is observed, 25% cross-reactivity with recombinant human (rh) EphA10 is observed, and no cross-reactivity with rhEphA1, 2, 5, rhEphB3, 4, rmEphA1, 3, 4, 6, 7, 8, rmEphB2, 3, recombinant rat (rr) EphA5, or rrEphB1 is observed.
Monoclonal Mouse IgG2B Clone # 465323
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human EphB6
Accession # O15197
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human EphB6 Fc Chimera (Catalog # 3384-B6)
Recombinant Mouse EphB6 Fc Chimera (Catalog # 611-B6)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphB6

EphB6, also known as Hep and Mep, is a 110 kDa type I transmembrane glycoprotein in the Eph receptor tyrosine kinase family. EphB6 is expressed in brain, pancreas, thymus, kidney, and on peripheral T cells. Depending on concentration, Ephrin-B2 can either inhibit or promote EphB6 mediated cell adhesion and migration. EphB6 participates in T cell activation, and its level of expression is inversely correlated with tumor aggressiveness in a variety of malignancies. Within the ECD, human EphB6 shares 91% aa sequence identity with mouse and rat EphB6.

Long Name
Eph Receptor B6
Entrez Gene IDs
2051 (Human); 13848 (Mouse)
Alternate Names
EC; EPH receptor B6; EphB6; ephrin type-B receptor 6; Hep; HEPTyrosine-protein kinase-defective receptor EPH-6; Mep; MGC129910; MGC129911

Product Datasheets


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human/Mouse EphB6 Antibody
By Anonymous on 12/07/2020
Application: Microarray Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma