Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 465323
View all EphB6 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human EphB6
Val17-Thr579
Accession # O15197

Specificity

Detects human and mouse EphB6 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) EphB6 is observed, 25% cross-reactivity with recombinant human (rh) EphA10 is observed, and no cross-reactivity with rhEphA1, 2, 5, rhEphB3, 4, rmEphA1, 3, 4, 6, 7, 8, rmEphB2, 3, recombinant rat (rr) EphA5, or rrEphB1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human/Mouse EphB6 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human EphB6 Fc Chimera (Catalog # 3384-B6)
Recombinant Mouse EphB6 Fc Chimera (Catalog # 611-B6)

Reviewed Applications

Read 1 review rated 5 using MAB3384 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EphB6

EphB6, also known as Hep and Mep, is a 110 kDa type I transmembrane glycoprotein in the Eph receptor tyrosine kinase family. EphB6 is expressed in brain, pancreas, thymus, kidney, and on peripheral T cells. Depending on concentration, Ephrin-B2 can either inhibit or promote EphB6 mediated cell adhesion and migration. EphB6 participates in T cell activation, and its level of expression is inversely correlated with tumor aggressiveness in a variety of malignancies. Within the ECD, human EphB6 shares 91% aa sequence identity with mouse and rat EphB6.

Long Name

Eph Receptor B6

Alternate Names

Hep, Mep

Entrez Gene IDs

2051 (Human); 13848 (Mouse)

Gene Symbol

EPHB6

UniProt

O15197

Additional EphB6 Products

Product Documents for Human/Mouse EphB6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse EphB6 Antibody

For research use only

Customer Reviews for Human/Mouse EphB6 Antibody (1)

5 out of 5
1 Customer Rating
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  • Human/Mouse EphB6 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human/Mouse EphB6 Antibody MAB3384

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