Detection of Human and Mouse FosB/G0S3 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with XXX TPA for 2 hours and NIH‑3T3 mouse embryonic fibroblast cell line untreated or treated with serum for 1 hour. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse FosB/G0S3 Monoclonal Antibody (Catalog # MAB2214), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FosB/G0S3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
FosB (FBJ murine osteoscarcoma viral oncogene homolog B) is one of four Fos family proteins that associate with Jun family proteins to form the AP-1 transcription factor complex. AP-1 activity is important for gene transcription in a wide variety of cellular processes.
FBJ Murine Osteosarcoma Viral Oncogene Homolog B
Entrez Gene IDs:
2354 (Human); 14282 (Mouse)
activator protein 1; AP-1; DKFZp686C0818; FBJ murine osteosarcoma viral oncogene homolog B; FosB; G0S3; G0S3G0/G1 switch regulatory protein 3; GOS3; GOSB; MGC42291; oncogene FOS-B; protein fosB
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