Detection of Human and Mouse HSP47 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, A549 human lung carcinoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse HSP47 Monoclonal Antibody (Catalog # MAB9166) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HSP47 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
HSP47 in Human Breast Cancer Tissue.
HSP47 was detected in immersion fixed paraffin-embedded sections of human normal breast (left) and breast cancer tissue (right) using Mouse Anti-Human/Mouse HSP47 Monoclonal Antibody (Catalog # MAB9166) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Heat Shock Protein 47 (HSP47), also known as Serpin-H1/CBP1/CBP2, is localized to endoplasmic reticulum (ER),
where it is a collagen-specific molecular chaperone. In the ER, HSP47 interacts with and
stabilizes correctly-folded procollagen. Nucleotide polymorphisms may be
associated with preterm birth and Osteogenesis Imperfecta type X. Serpin-H1 is up-regulated in several cancers
including squamous cell carcinoma, breast and prostate carcinomas. Expression in tumors drives malignant growth
and invasion by enhancing deposition of extracellular matrix proteins.
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Tasab M, et al. (2000) EMBO J. 19:22043.
Kwon YJ, et al. (2009) Oncol Res. 18:1414.
Zhu J, et al. (2015) Cancer Res. 75:15805.
Nese N, et al. (2010) Anal Quant Cytol Histol. 32:90.
Heat Shock Protein 47
Entrez Gene IDs:
871 (Human); 12406 (Mouse); 29345 (Rat)
(collagen binding protein 1); Arsenic-transactivated protein 3; AsTP3; BERF-1; CBP1; CBP2; CBP2RA-A47; Cell proliferation-inducing gene 14 protein; Collagen-binding protein; colligen; Colligin; colligin-1; colligin-2; gp46; HSP47; HSP47serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47); member 1, (collagen binding protein 1); member 2; member 2, (collagen-binding protein 2); OI10; PPROM; proliferation-inducing gene 14; RA-A47; rheumatoid arthritis antigen A-47,47 kDa heat shock protein; Rheumatoid arthritis-related antigen RA-A47; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47); Serpin H1; serpin peptidase inhibitor, clade H (heat shock protein 47), member 1; SERPINH1; SERPINH2colligin
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