Human/Mouse KLF5 Antibody

(3 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse KLF5 in Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) KLF4, rhKLF6, recombinant mouse (rm) KLF4 and rmKLF15 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human KLF5
    Leu85-Ala177
    Accession # Q13887
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse KLF5 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells, D3 mouse embryonic stem cell line, and P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human KLF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3758) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for KLF5 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
KLF5 in D3 Mouse Stem Cells. KLF5 was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human KLF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3758) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Mouse KLF5 by Simple WesternTM. Simple Western lane view shows lysates of D3 mouse embryonic stem cell line, loaded at 0.2 mg/mL. A specific band was detected for KLF5 at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse KLF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3758) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: KLF5
Human KLF5, also known as basic transcription element binding protein 2 (BTEB2), is a widely expressed 51 kDa (457 aa) member of the kruppel C2H2-type zinc finger protein family. KLF5 contains three C2H2-type zinc fingers at the carboxyl-terminus that preferentially bind to cis-DNA elements that are GC rich. It is a transcription activator that promotes cell proliferation and oncogenesis. Over the region used as immunogen, human, mouse and canine KLF5 share 95% aa sequence identity.
  • Long Name:
    Kruppel-Like Factor 5
  • Entrez Gene IDs:
    688 (Human)
  • Alternate Names:
    Basic transcription element-binding protein 2; BTEB2; BTE-binding protein 2; CKLFGC-box-binding protein 2; Colon krueppel-like factor; colon kruppel-like factor; GC box binding protein 2; IKLFbasic transcription element binding protein 2; Intestinal-enriched krueppel-like factor; intestinal-enriched kruppel-like factor; KLF5; Krueppel-like factor 5; Kruppel-like factor 5 (intestinal); Transcription factor BTEB2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. KLF13 regulates the differentiation-dependent human papillomavirus life cycle in keratinocytes through STAT5 and IL-8
    Authors: W Zhang, S Hong, KP Maniar, S Cheng, C Jie, AW Rademaker, AM Krensky, C Clayberger
    Oncogene, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. The intestinal epithelial cell differentiation marker intestinal alkaline phosphatase (ALPi) is selectively induced by histone deacetylase inhibitors (HDACi) in colon cancer cells in a Kruppel-like factor 5 (KLF5)-dependent manner.
    Authors: Shin J, Carr A, Corner G, Togel L, Davaos-Salas M, Tran H, Chueh A, Al-Obaidi S, Chionh F, Ahmed N, Buchanan D, Young J, Malo M, Hodin R, Arango D, Sieber O, Augenlicht L, Dhillon A, Weber T, Mariadason J
    J Biol Chem, 2014;289(36):25306-16.
    Species: Human
    Sample Type: Cell Lysates
    Application: ChIP
  3. Epithelial microRNAs regulate gut mucosal immunity via epithelium-T cell crosstalk.
    Authors: Biton M, Levin A, Slyper M, Alkalay I, Horwitz E, Mor H, Kredo-Russo S, Avnit-Sagi T, Cojocaru G, Zreik F, Bentwich Z, Poy MN, Artis D, Walker MD, Hornstein E, Pikarsky E, Ben-Neriah Y
    Nat. Immunol., 2011;12(3):239-46.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
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