Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all KLF5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human KLF5
Leu85-Ala177
Accession # Q13887

Specificity

Detects human and mouse KLF5 in Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) KLF4, rhKLF6, recombinant mouse (rm) KLF4 and rmKLF15 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse KLF5 Antibody

Detection of Human and Mouse KLF5 antibody by Western Blot.

Detection of Human and Mouse KLF5 by Western Blot.

Western blot shows lysates of BG01V human embryonic stem cells, D3 mouse embryonic stem cell line, and P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human KLF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3758) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for KLF5 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

KLF5 antibody in D3 Mouse Stem Cells by Immunocytochemistry (ICC).

KLF5 in D3 Mouse Stem Cells.

KLF5 was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human KLF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3758) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Mouse KLF5 antibody by Simple WesternTM.

Detection of Mouse KLF5 by Simple WesternTM.

Simple Western lane view shows lysates of D3 mouse embryonic stem cell line, loaded at 0.2 mg/mL. A specific band was detected for KLF5 at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse KLF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3758) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Shows Human KLF5 Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human KLF5 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and KLF5 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse KLF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3758) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for KLF5 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse KLF5 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells and D3 mouse embryonic stem cells

Knockout Validated

KLF5 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in KLF5 knockout HeLa cell line.

Simple Western

10 µg/mL
Sample: D3 mouse embryonic stem cell line

Western Blot

1 µg/mL
Sample: BG01V human embryonic stem cells, D3 mouse embryonic stem cell line, and P19 mouse embryonal carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KLF5

Human KLF5, also known as basic transcription element binding protein 2 (BTEB2), is a widely expressed 51 kDa (457 aa) member of the kruppel C2H2-type zinc finger protein family. KLF5 contains three C2H2-type zinc fingers at the carboxyl-terminus that preferentially bind to cis-DNA elements that are GC rich. It is a transcription activator that promotes cell proliferation and oncogenesis. Over the region used as immunogen, human, mouse and canine KLF5 share 95% aa sequence identity.

Long Name

Kruppel-Like Factor 5

Alternate Names

BTEB2

Entrez Gene IDs

688 (Human)

Gene Symbol

KLF5

UniProt

Q13887

Additional KLF5 Products

Product Documents for Human/Mouse KLF5 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse KLF5 Antibody

For research use only

Citations for Human/Mouse KLF5 Antibody

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Protocols

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