Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MSX1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MSX1
Met1-Thr165
Accession # P28360

Specificity

Detects human and mouse MSX1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse MSX1 Antibody

Detection of Human MSX1 antibody by Western Blot.

Detection of Human MSX1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.1 µg/mL Goat Anti-Human/Mouse MSX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5045) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for MSX1 was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MSX1 antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P).

MSX1 in Human Ovarian Cancer Tissue.

MSX1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human/Mouse MSX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5045) at 0.3, 1.0 and 3.0 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human MSX1 by Western Blot

Detection of Human MSX1 by Western Blot

Regulation of MSX1 expression is mediated by pSMAD1/5/8 in the human cap-stage tooth germs. (A–C) Immunostaining shows that MSX1 expression is abundant in dimethyl sulfoxide (DMSO)-treated human cap-stage tooth germs, dramatically reduced in dorsomorphin treated human cap-stage tooth germ, and unaltered in U0126 + SB203580 + SP600125 treated human cap-stage tooth germs. (D) A Western blot assay confirms the dramatically reduced expression of MSX1 in dorsomorphin treated human cap-stage tooth germs. Actin was used as the internal control. (E) Quantitative analysis of the Western blot assay. de, the dental epithelium; dm, the dental mesenchyme. Error bars represent SD. ***p < 0.001. Bar = 50 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35211032), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse MSX1 by Immunohistochemistry

Detection of Mouse MSX1 by Immunohistochemistry

Msx1 expression in E12.5 Wls-knockout mutant and control cerebella. In control cerebellum (A,B), Msx1 is expressed in the RL. Expression of Msx1 in the RL is persisted in the Wls knockout cerebellum (C,D) and similar to Msx1 expression in the control RL. RL, rhombic lip. Scale bars, 100 μm. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fnmol.2024.1356544/full), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse MSX1 by Immunohistochemistry

Detection of Mouse MSX1 by Immunohistochemistry

Msx1 and Msx3 expression in E12.5 Atoh1-null and wildtype cerebella. (A,B) The FISH double-labeling of Msx1 and Msx3 in the E12.5 Atoh1-null (a,a’) and wildtype cerebella (b,b’). (a’ and b’) illustrate that the sections from mutant and wildtype are collected at the same medio-lateral position. (C–F) Expression of Msx1 and Msx3 shifts and now sharing a boundary in the Atoh1-null cerebellum as denoted by the red arrow in (C). Panels (A,C–F) indicate Msx1 and Msx3 expression persistence in the Atoh1-null cerebellum. The shift of Msx1 and Msx3 expression, however, does not result in cells that co-express Msx1 and Msx3 (A,C–E). (G–I) A very different picture is shown in the wildtype cerebellum where there is a notable gap between cells that express Msx3 and Msx1 as noted by white arrow in (G). (F,J) DAPI (blue) used as a counterstain. RL, Rhombic Lip; VZ, ventricular zone. All scale bars, 100 μm. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fnmol.2024.1356544/full), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse MSX1 by Immunohistochemistry

Detection of Mouse MSX1 by Immunohistochemistry

Temporal and spatial expression of Msx genes in the developing cerebellum. (A–C) Graphs show the dynamic nature of Msx expression in the cerebellum across 12 developmental time-points as observed from the RIKEN FANTOM5 transcriptome time-course data. (D–I) Sagittal sections of the RL with the right-side of panels denoting posterior and the bottom-side denoting ventral, with RNA in situ hybridization showing Msx genes expressed in the progenitor zones in (D–F) E11.5 and (G–I) E12.5. Msx1 expression is limited to the RL (white arrows in D,G) whereas Msx3 is limited to the VZ (black arrows in E,H). Msx2 expression is detected in the neuroepithelium but the boundary of the expression is not clear (F,I). See supplementary Figure S2 for negative control staining. RL, Rhombic Lip; VZ, Ventricular Zone. Scale bar, 100 μm. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fnmol.2024.1356544/full), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse MSX1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue

Western Blot

0.1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Reviewed Applications

Read 3 reviews rated 4.7 using AF5045 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MSX1

MSX1 (Msh homeobox homology 1) is a member of the muscle segment homoebox gene family. MSX1 is involved in limb-pattern formation, craniofacial development, odontogenesis, and tumor growth inhibition. MSX1 functions as a transcriptional repressor. MSX1 has been shown to interact with the linker histone, H1B, and repress transcription of the MyoD promoter. Chromosomal abnormalities involving MSX1 have been associated with the Wolf-Hirschhorn syndrome characterized by heart defects and mental retardation.

Long Name

Msh Homeobox 1

Alternate Names

HOX7, HYD1, OFC5

Entrez Gene IDs

4487 (Human); 17701 (Mouse); 81710 (Rat)

Gene Symbol

MSX1

UniProt

P28360

Additional MSX1 Products

Product Documents for Human/Mouse MSX1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse MSX1 Antibody

For research use only

Citations for Human/Mouse MSX1 Antibody

Customer Reviews for Human/Mouse MSX1 Antibody (3)

4.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
33%
3 Stars
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Showing  1 - 3 of 3 reviews Showing All
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  • Human/Mouse MSX1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: E12.5 mouse embryo fixed in 4% PFA
    Species: Mouse
    Verified Customer | Posted 06/23/2021
    Fixed 4% PFA overnight. Blocked with 1% BSA Primary antibody dilution - 1:20 Secondary antibody - Invitrogen Alexa Fluor 488 Secondary antibody dilution - 1:1000 Stained on a E12.5 mouse heart section.
    Human/Mouse MSX1 Antibody AF5045
  • Human/Mouse MSX1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: mouse digits
    Species: Mouse
    Verified Customer | Posted 12/17/2018
    Primary antibody was used at 1:100 and incubated at 4°C overnight.
    Human/Mouse MSX1 Antibody AF5045
  • Human/Mouse MSX1 Antibody
    Name: Gufa Lin
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: digit section
    Species: Mouse
    Verified Customer | Posted 12/17/2018
    Msx1 expression on frozen sections of regenerating mouse digit was detected with AF5045 (1:200) incubated overnight in fridge ( 4°C) in PBS/3% BSA/0.1% Triton-X100, secondary antibody (1:500) incubated at room temperature for 90 minutes, followed by nuclei counterstaining with DAPI.
    Human/Mouse MSX1 Antibody AF5045

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