Human/Mouse MSX1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human MSX1 by Western Blot.
2 Images
Product Details
Citations (1)
Supplemental Products

Human/Mouse MSX1 Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse MSX1 in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human MSX1
Accession # P28360
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human MSX1 by Western Blot. View Larger

Detection of Human MSX1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 30 μg of whole cell lysate (WCL), 20 μg of cytoplasmic (Cyto), and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 0.1 µg/mL Goat Anti-Human/Mouse MSX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5045) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for MSX1 was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry MSX1 in Human Ovarian Cancer Tissue. View Larger

MSX1 in Human Ovarian Cancer Tissue. MSX1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human/Mouse MSX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5045) at 0.3, 1.0 and 3.0 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSX1

MSX1 (Msh homeobox homology 1) is a member of the muscle segment homoebox gene family. MSX1 is involved in limb-pattern formation, craniofacial development, odontogenesis, and tumor growth inhibition. MSX1 functions as a transcriptional repressor. MSX1 has been shown to interact with the linker histone, H1B, and repress transcription of the MyoD promoter. Chromosomal abnormalities involving MSX1 have been associated with the Wolf-Hirschhorn syndrome characterized by heart defects and mental retardation.

Long Name
Msh Homeobox 1
Entrez Gene IDs
4487 (Human); 17701 (Mouse); 81710 (Rat)
Alternate Names
Homeobox protein Hox-7; homeobox protein MSX-1; HOX7; HOX7homeobox 7; HYD1; HYD1msh homeobox homolog 1 (Drosophila); msh (Drosophila) homeo box homolog 1 (formerly homeo box 7); msh homeo box 1; msh homeobox 1; Msh homeobox 1-like protein; msh homeobox homolog 1; MSX1; OFC5; STHAG1

Product Datasheets

Citation for Human/Mouse MSX1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A new strategy to measure intercellular adhesion forces in mature cell-cell contacts
    Authors: A Sancho, I Vandersmis, S Craps, A Luttun, J Groll
    Sci Rep, 2017;7(0):46152.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB


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Cell and Tissue Staining Kits

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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