Human/Mouse NLRP3/NALP3 Antibody

Catalog # Availability Size / Price Qty
MAB7578
MAB7578-SP
Human/Mouse NLRP3/NALP3 Antibody in Data
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Product Details
Citations (23)
FAQs
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Reviews (3)

Human/Mouse NLRP3/NALP3 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse NLRP3/NALP3 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human NLRP3/NALP3 is observed.
Source
Monoclonal Rat IgG2A Clone # 768319
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse NLRP3/NALP3
Met1-Arg153
Accession # Q8R4B8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse NLRP3/NALP3 antibody by Western Blot. View Larger

Detection of Mouse NLRP3/NALP3 by Western Blot. Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB7578) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for NLRP3/NALP3 at approximately 117 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Catalog # AF6789is recommended for use with human lysates.

Immunocytochemistry NLRP3/NALP3 antibody in RAW 264 by Immunocytochemistry (ICC).7 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

NLRP3/NALP3 in RAW 264.7 Mouse Cell Line. NLRP3/NALP3 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Human/Mouse NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB7578) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry NLRP3/NALP3 antibody in Human Tonsil Tissue by Immunohistochemistry (IHC-P). View Larger

NLRP3/NALP3 in Human Tonsil Tissue. NLRP3/NALP3 was detected in immersion fixed paraffin-embedded sections of human tonsil tissue using Rat Anti-Human/Mouse NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB7578) at 5 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Intracellular Staining by Flow Cytometry Detection of NLRP3/NALP3 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of NLRP3/NALP3 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse NLRP3/ NALP3 Monoclonal Antibody (Catalog # MAB7578, filled histo-gram) or isotype control antibody (Catalog # MAB006, open histo-gram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Intracellular Staining by Flow Cytometry Detection of NLRP3/NALP3 antibody in Human Monocytes antibody by Flow Cytometry. View Larger

Detection of NLRP3/NALP3 in Human Monocytes by Flow Cytometry. Human peripheral blood monocytes was stained with Rat Anti-Human/Mouse NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB7578, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NLRP3/NALP3

NLRP3, also known as NALP3, CIAS1, PYPAF or Cryopyrin, is a cytosolic ~120 kDa member of the NLRP family of proteins expressed in leukocytes, especially neutrophils. As a component of the inflammasome, NLRP3 activates caspases 1 and 5. Defects in NLRP3 may cause FCAS1, CINCA, or Muckle-Wells autoinflammatory syndromes. Mouse NLRP3 contains an N-terminal Pyrin domain (aa 1-91) followed by a Nacht region (aa 216-532), and seven LRRs (aa 739-988). Within the sequence used as an immunogen, mouse NLRP3 shares 78% and 93% aa identity with human and rat NLRP3, respectively. Alternate splicing of mouse NLRP3 generates additional isoforms that lack either LRR2 and 3, LRR 6 and 7, or LRR4-9.

Long Name
NLR Family, Pyrin Domain Containing 2
Entrez Gene IDs
114548 (Human); 216799 (Mouse); 287362 (Rat)
Alternate Names
AGTAVPRL; AII; AII/AVP; Angiotensin/vasopressin receptor AII/AVP-like; AVP; C1orf7; Caterpiller protein 1.1; CIAS1; CLR1.1; Cold autoinflammatory syndrome 1 protein; Cold autoinflammatory syndrome 1; Cryopyrin; Familial cold autoinflammatory syndrome; FCAS; FCU; Leucine-rich repeat-, and PYD-containing protein 3; Muckle-Wells syndrome; MWS; NACHT; NACHT, LRR and PYD containing protein 3; NACHT, LRR and PYD domains-containing protein 3; NALP3; NLR family, pyrin domain containing 3; NLRP3; Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3; PYPAF1; PYRIN-containing APAF1-like protein 1

Product Datasheets

Citations for Human/Mouse NLRP3/NALP3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. AIM2 inflammasome activation may mediate high mobility group box 1 release in murine allergic rhinitis
    Authors: Y Wang, S Chen, PL Yang, JJ Chen, WJ Kong, YJ Wang
    Brazilian journal of otorhinolaryngology, 2021;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi�strains unveil a role for fibroblasts in neutrophil recruitment and activation
    Authors: AER Oliveira, MCA Pereira, AT Belew, LRP Ferreira, LMN Pereira, EGA Neves, MDCP Nunes, BA Burleigh, WO Dutra, NM El-Sayed, RT Gazzinelli, SMR Teixeira
    PLoS Pathog., 2020;16(8):e1008781.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  3. The Combination of Sulforaphane and Fernblock� XP Improves Individual Beneficial Effects in Normal and Neoplastic Human Skin Cell Lines
    Authors: S Serini, R Guarino, R Ottes Vasc, L Celleno, G Calviello
    Nutrients, 2020;12(6):.
    Species: Human
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  4. Inhibition of the NLRP3-inflammasome prevents cognitive deficits in experimental autoimmune encephalomyelitis mice via the alteration of astrocyte phenotype
    Authors: B Hou, Y Zhang, P Liang, Y He, B Peng, W Liu, S Han, J Yin, X He
    Cell Death Dis, 2020;11(5):377.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. NLRP3 Inflammasome and Mineralocorticoid Receptors Are Associated with Vascular Dysfunction in Type 2 Diabetes Mellitus
    Authors: NS Ferreira, T Bruder-Nas, CA Pereira, CZ Zanotto, DS Prado, JF Silva, DM Rassi, MC Foss-Freit, JC Alves-Filh, D Carlos, RC Tostes
    Cells, 2019;8(12):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. DDX3X acts as a live-or-die checkpoint in stressed cells by regulating NLRP3 inflammasome
    Authors: P Samir, S Kesavardha, DM Patmore, S Gingras, RKS Malireddi, R Karki, CS Guy, B Briard, DE Place, A Bhattachar, BR Sharma, A Nourse, SV King, A Pitre, AR Burton, S Pelletier, RJ Gilbertson, TD Kanneganti
    Nature, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. Magnesium Sulfate Mitigates the Progression of Monocrotaline Pulmonary Hypertension in Rats
    Authors: CY Chang, HJ Shih, IT Huang, PS Tsai, KY Chen, CJ Huang
    Int J Mol Sci, 2019;20(18):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Downregulation of the NLRP3 inflammasome by adiponectin rescues Duchenne muscular dystrophy
    Authors: R Boursereau, M Abou-Samra, S Lecompte, L Noel, SM Brichard
    BMC Biol., 2018;16(1):33.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Endothelial GPR124 Exaggerates the Pathogenesis of Atherosclerosis by Activating Inflammation
    Authors: DM Gong, YL Zhang, DY Chen, LJ Hong, F Han, QB Liu, JJ Jiang, YM Lu
    Cell. Physiol. Biochem., 2018;45(2):547-557.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Eruca sativa seed extract: A novel natural product able to counteract neuroinflammation
    Authors: A Gugliandol, S Giacoppo, M Ficicchia, A Aliquò, P Bramanti, E Mazzon
    Mol Med Rep, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  11. Issues with the Specificity of Immunological Reagents for NLRP3: Implications for Age-related Macular Degeneration
    Authors: C Kosmidou, NE Efstathiou, MV Hoang, S Notomi, EK Konstantin, M Hirano, K Takahashi, DE Maidana, P Tsoka, L Young, ES Gragoudas, TW Olsen, Y Morizane, JW Miller, DG Vavvas
    Sci Rep, 2018;8(1):461.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Evidence that TLR4 Is Not a Receptor for Saturated Fatty Acids but Mediates Lipid-Induced Inflammation by Reprogramming Macrophage Metabolism
    Authors: GI Lancaster, KG Langley, NA Berglund, HL Kammoun, S Reibe, E Estevez, J Weir, NA Mellett, G Pernes, JRW Conway, MKS Lee, P Timpson, AJ Murphy, SL Masters, S Gerondakis, N Bartonicek, DC Kaczorowsk, ME Dinger, PJ Meikle, PJ Bond, MA Febbraio
    Cell Metab., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Inhibition of NLRP3 Inflammasome Pathway by Butyrate Improves Corneal Wound Healing in Corneal Alkali Burn
    Authors: F Bian, Y Xiao, M Zaheer, EA Volpe, SC Pflugfelde, DQ Li, CS de Paiva
    Int J Mol Sci, 2017;18(3):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  14. Intestinal fungi contribute to development of alcoholic liver disease
    Authors: AM Yang, T Inamine, K Hochrath, P Chen, L Wang, C Llorente, S Bluemel, P Hartmann, J Xu, Y Koyama, T Kisseleva, MG Torralba, K Moncera, K Beeri, CS Chen, K Freese, C Hellerbran, SM Lee, HM Hoffman, WZ Mehal, G Garcia-Tsa, EA Mutlu, A Keshavarzi, GD Brown, SB Ho, R Bataller, P Stärkel, DE Fouts, B Schnabl
    J. Clin. Invest., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. NLRP3 signaling drives macrophage-induced adaptive immune suppression in pancreatic carcinoma
    Authors: D Daley, VR Mani, N Mohan, N Akkad, GSDB Pandian, S Savadkar, KB Lee, A Torres-Her, B Aykut, B Diskin, W Wang, MS Farooq, AI Mahmud, G Werba, EJ Morales, S Lall, BJ Wadowski, AG Rubin, ME Berman, R Narayanan, M Hundeyin, G Miller
    J. Exp. Med., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. The Antimalarial Chloroquine Suppresses LPS-Induced NLRP3 Inflammasome Activation and Confers Protection against Murine Endotoxic Shock
    Authors: X Chen, N Wang, Y Zhu, Y Lu, X Liu, J Zheng
    Mediators Inflamm., 2017;2017(0):6543237.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Expression of TLR2, NOD1, and NOD2 and the NLRP3 Inflammasome in Renal Tubular Epithelial Cells of Male versus Female Mice
    Authors: SE DeWolf, AA Shigeoka, A Scheinok, SG Kasimsetty, AK Welch, DB McKay
    Nephron, 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Knockout of Ccr2 alleviates photoreceptor cell death in rodent retina exposed to chronic blue light
    Cell Death Dis, 2016;7(11):e2468.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  19. Suppression of NLRP3 inflammasome by gamma-tocotrienol ameliorates type 2 diabetes.
    Authors: Kim Y, Wang W, Okla M, Kang I, Moreau R, Chung S
    J Lipid Res, 2015;57(1):66-76.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Caspase-8 as an Effector and Regulator of NLRP3 Inflammasome Signaling.
    Authors: Antonopoulos C, Russo H, El Sanadi C, Martin B, Li X, Kaiser W, Mocarski E, Dubyak G
    J Biol Chem, 2015;290(33):20167-84.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. The cyclopentenone prostaglandin 15d-PGJ2 inhibits the NLRP1 and NLRP3 inflammasomes.
    Authors: Maier N, Leppla S, Moayeri M
    J Immunol, 2015;194(6):2776-85.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  22. Chinese Herbal Preparation Xuebijing Potently Inhibits Inflammasome Activation in Hepatocytes and Ameliorates Mouse Liver Ischemia-Reperfusion Injury.
    Authors: Liu X, Hu Z, Zhou B, Li X, Tao R
    PLoS ONE, 2015;10(7):e0131436.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  23. ASC in renal collecting duct epithelial cells contributes to inflammation and injury after unilateral ureteral obstruction.
    Authors: Komada T, Usui F, Shirasuna K, Kawashima A, Kimura H, Karasawa T, Nishimura S, Sagara J, Noda T, Taniguchi S, Muto S, Nagata D, Kusano E, Takahashi M
    Am J Pathol, 2014;184(5):1287-98.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human/Mouse NLRP3/NALP3 Antibody

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Human/Mouse NLRP3/NALP3 Antibody
By Anonymous on 07/06/2021
Application: ICC/IF Sample Tested: Connective tissue Species: Rat

Human/Mouse NLRP3/NALP3 Antibody
By Anonymous on 07/21/2017
Application: ICC/IF Sample Tested: J774A.1 mouse reticulum cell sarcoma macrophage cell line Species: Mouse

Human/Mouse NLRP3/NALP3 Antibody
By Anonymous on 01/11/2017
Application: WB Sample Tested: primary macrophages Species: Mouse