Pax3/Pax7 /Pax7 Antibody (274212)

R&D Systems | Catalog # MAB2457

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Porcine

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP), Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 274212
View all Pax3/Pax7 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Pax3 (isoform Pax3a)
Met1-Ser215
Accession # NP_000429

Specificity

Detects human and mouse Pax3 and Pax7 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Pax1 is observed, approximately 10% with rhPax6, and less than 2% with rhPax2, rhPax4, rhPax5, and rhPax9.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Pax3/Pax7 /Pax7 Antibody (274212)

Detection of Pax3/Pax7 in B16-F1 cells by Flow Cytometry

B16-F1 cells were stained with Mouse Anti-Human/Mouse Pax3/Pax7 /Pax7 Monoclonal Antibody (Catalog # MAB2457, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Pax3 antibody in B16-F1 Mouse Cell Line by Immunocytochemistry (ICC).

Pax3/Pax7 in B16‑F1 Mouse Cell Line.

Pax3/Pax7 was detected in immersion fixed B16-F1 mouse melanoma cell line using Mouse Anti-Human/Mouse Pax3/Pax7 Monoclonal Antibody (Catalog # MAB2457) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Pax3/Pax7 by Immunocytochemistry/Immunofluorescence

Detection of Human Pax3/Pax7 by Immunocytochemistry/Immunofluorescence

Characterization of iMPCs during monolayer differentiation. a–e Representative immunostaining of Pax3 (a), Myf5 (b), MyoD (c), and MyoG (d), and corresponding quantification (e) during iMPC expansion. Scale bar=100 µm. f Representative FACS analysis for CD56 in H9 and TRiPSC derived iMPCs. g Representative immunostaining (top) and quantification (bottom) of Pax7+ and MyoG+ cell populations for H9 and TRiPS derived myotubes at 2 weeks of monolayer differentiation. (n = 6 samples from 2 differentiations for each cell line). h Representative immunostaining and quantification of GFP+/Pax7+ and GFP-/Pax7+ cell pools at 2 weeks of monolayer differentiation. Scale bar=50 µm. (n = 4 samples from 2 differentiations for each cell line). i Representative immunostaining and quantification of myotube diameter at 1, 2, and 4 weeks of monolayer differentiation. (*P < 0.05 vs. 1 week, #P < 0.05 vs. 4 week, Tukey–Kramer HSD test; n = 6 samples from 2 differentiations for each cell line). Scale bars=50 µm. Data are presented as mean ± SEM Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29317646), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Pax3/Pax7/Pax7 by Immunohistochemistry

Detection of Pax3/Pax7/Pax7 by Immunohistochemistry

Immunofluorescent analysis of Pax3 expression in the developing mouse cerebellum.(A) Bar plot showing the percentage of Pax3 + cells co-stained (y-axis) with cerebellar cell markers Pax2, Foxp2 and Calbindin at E12, E15, and P3 (x-axis). (B) Top: Immunofluorescent co-staining of Pax3 (red) and Foxp2 (green) in embryonic cerebellum at E15. Merged image is a composite image of the Pax3, Foxp2, and DAPI. Bottom: Immunofluorescent co-staining of Pax3 (red) and Calb (green) in the postnatal cerebellum at P0. Merged image is a composite image of the Pax3, Foxp2, and DAPI. Labels: VZ: Ventricular zone, EGL: External granular layer, PCL: Purkinje cell layer, ML: Molecular layer, Scalebars = 100 µm. (C) Immunofluorescent staining of Pax3 (red) in the developing cerebellum at P9. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35942939), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Pax3/Pax7/Pax7 by Immunohistochemistry

Detection of Pax3/Pax7/Pax7 by Immunohistochemistry

Immunofluorescent analysis of Pax3 expression in the developing mouse cerebellum.(A) Bar plot showing the percentage of Pax3 + cells co-stained (y-axis) with cerebellar cell markers Pax2, Foxp2 and Calbindin at E12, E15, and P3 (x-axis). (B) Top: Immunofluorescent co-staining of Pax3 (red) and Foxp2 (green) in embryonic cerebellum at E15. Merged image is a composite image of the Pax3, Foxp2, and DAPI. Bottom: Immunofluorescent co-staining of Pax3 (red) and Calb (green) in the postnatal cerebellum at P0. Merged image is a composite image of the Pax3, Foxp2, and DAPI. Labels: VZ: Ventricular zone, EGL: External granular layer, PCL: Purkinje cell layer, ML: Molecular layer, Scalebars = 100 µm. (C) Immunofluorescent staining of Pax3 (red) in the developing cerebellum at P9. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35942939), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Pax3/Pax7 /Pax7 Antibody (274212)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed B16-F1 mouse melanoma cell line

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: B16‑F1 mouse melanoma cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Human Pax3

Reviewed Applications

Read 2 reviews rated 4.5 using MAB2457 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Pax3/Pax7

Pax3 belongs to the family of paired box transcription factors. Pax family proteins typically contain a paired box domain and a paired-type homeodomain. These transcription factors play critical roles during fetal development. Human and mouse Pax3 share 98% amino acid sequence identity.

Long Name

Paired Box Gene 3 /Paired Box Gene 7

UniProt

NP_000429

Additional Pax3/Pax7 Products

Product Documents for Pax3/Pax7 /Pax7 Antibody (274212)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Pax3/Pax7 /Pax7 Antibody (274212)

For research use only

Citations for Pax3/Pax7 /Pax7 Antibody (274212)

Customer Reviews for Pax3/Pax7 /Pax7 Antibody (274212) (2)

4.5 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Pax3/Pax7 /Pax7 Antibody (274212)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Human/Mouse Pax3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: B16-F1 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 09/13/2021
    Pax3/Pax7 /Pax7 Antibody (274212) MAB2457
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 21078671
    Species: Other
    Verified Customer | Posted 02/19/2015

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies