Human/Mouse Pax3 Antibody

(8 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Pax3 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 274212
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Pax3 (isoform Pax3a)
    Met1-Ser215
    Accession # NP_000429
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human Pax3
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    0.25 µg/106 cells
    B16‑F1 mouse melanoma cell line fixed with paraformaldehyde and permeabilized with saponin
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Pax3 in B16‑F1 Mouse Cell Line. Pax3 was detected in immersion fixed B16‑F1 mouse melanoma cell line using Mouse Anti-Human/Mouse Pax3 Monoclonal Antibody (Catalog # MAB2457) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pax3
Pax3 belongs to the family of paired box transcription factors. Pax family proteins typically contain a paired box domain and a paired-type homeodomain. These transcription factors play critical roles during fetal development. Human and mouse Pax3 share 98% amino acid sequence identity.
  • Long Name:
    Paired Box Gene 3 [Waardenburg Syndrome 1]
  • Entrez Gene IDs:
    5077 (Human); 18505 (Mouse)
  • Alternate Names:
    CDHS; HUP2; HUP2MGC120384; MGC120381; MGC120382; MGC120383; MGC134778; paired box 3; paired box gene 3 (Waardenburg syndrome 1); paired box homeotic gene 3; paired box protein Pax-3; paired domain gene 3; paired domain gene HuP2; Pax3; Waardenburg syndrome 1; WS1; WS3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. microRNA-155, induced by interleukin-1ss, represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells.
    Authors: Arts N, Cane S, Hennequart M, Lamy J, Bommer G, Van den Eynde B, De Plaen E
    PLoS ONE, 2015;10(4):e0122517.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Pax3 and Zic1 trigger the early neural crest gene regulatory network by the direct activation of multiple key neural crest specifiers.
    Authors: Plouhinec J, Roche D, Pegoraro C, Figueiredo A, Maczkowiak F, Brunet L, Milet C, Vert J, Pollet N, Harland R, Monsoro-Burq A
    Dev Biol, 2014;386(2):461-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  3. Lineage of origin in rhabdomyosarcoma informs pharmacological response.
    Authors: Abraham J, Nunez-Alvarez Y, Hettmer S, Carrio E, Chen H, Nishijo K, Huang E, Prajapati S, Walker R, Davis S, Rebeles J, Wiebush H, McCleish A, Hampton S, Bjornson C, Brack A, Wagers A, Rando T, Capecchi M, Marini F, Ehler B, Zarzabal L, Goros M, Michalek J, Meltzer P, Langenau D, LeGallo R, Mansoor A, Chen Y, Suelves M, Rubin B, Keller C
    Genes Dev, 2014;28(14):1578-91.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. Cell-cycle dependent expression of a translocation-mediated fusion oncogene mediates checkpoint adaptation in rhabdomyosarcoma.
    Authors: Kikuchi K, Hettmer S, Aslam M, Michalek J, Laub W, Wilky B, Loeb D, Rubin B, Wagers A, Keller C
    PLoS Genet, 2014;10(1):e1004107.
  5. Functional dissection of Pax3 in paraxial mesoderm development and myogenesis.
    Authors: Magli A, Schnettler E, Rinaldi F, Bremer P, Perlingeiro R
    Stem Cells, 2013;31(1):59-70.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. Selective development of myogenic mesenchymal cells from human embryonic and induced pluripotent stem cells.
    Authors: Awaya, Tomonari, Kato, Takeo, Mizuno, Yuta, Chang, Hsi, Niwa, Akira, Umeda, Katsutsu, Nakahata, Tatsutos, Heike, Toshio
    PLoS ONE, 2012;7(12):e51638.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  7. MyoD directly up-regulates premyogenic mesoderm factors during induction of skeletal myogenesis in stem cells.
    Authors: Gianakopoulos PJ, Mehta V, Voronova A, Cao Y, Yao Z, Coutu J, Wang X, Waddington MS, Tapscott SJ, Skerjanc IS
    J. Biol. Chem., 2011;286(4):2517-25.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  8. Pax3 activation promotes the differentiation of mesenchymal stem cells toward the myogenic lineage.
    Authors: Gang EJ, Bosnakovski D, Simsek T, To K, Perlingeiro RC
    Exp. Cell Res., 2008;314(8):1721-33.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
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