Human/Mouse Plexin C1 Antibody Summary
Accession # O60486
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Plexin C1 in Human Melanoma. Plexin C1 was detected in immersion fixed paraffin-embedded sections of human melanoma using Mouse Anti-Human Plexin C1 Monoclonal Antibody (Catalog # MAB3887) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Plexin C1 in Mouse Embryo. Plexin C1 was detected in immersion fixed frozen sections of mouse embryonic gut (12 d.p.c.) using Mouse Anti-Human Plexin C1 Monoclonal Antibody (Catalog # MAB3887) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Plexin C1
Plexin C1 (also VESPR and CD232) is a 210 kDa member of the plexin-C subfamily, plexin family of proteins. It is a type I transmembrane glycoprotein that is found on monocytes, dendritic cells and neutrophils. Plexin C1 binds Sema7A, and its viral counterpart, vaccinia semaphorin A39R. Ligation induces actin cytoskeletal rearrangement and cell immobilization. Mature human Plexin C1 is 1534 amino acids (aa) in length. It contains a 910 aa extracellular domain (ECD) and a 603 aa cytoplasmic region. The ECD contains one Sema-domain (aa 49-440), two PSI domains (aa 454-605) and two IPT regions (aa 664-842). Over aa 49-161, human Plexin C1 shares 92% aa identity with mouse Plexin C1.
Citations for Human/Mouse Plexin C1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Viral semaphorin inhibits dendritic cell phagocytosis and migration but is not essential for gammaherpesvirus-induced lymphoproliferation in malignant catarrhal fever.
Authors: Myster F, Palmeira L, Sorel O, Bouillenne F, DePauw E, Schwartz-Cornil I, Vanderplasschen A, Dewals B
J Virol, 2015;89(7):3630-47.
Sample Types: Whole Cells
Intestinal epithelial cell-derived semaphorin 7A negatively regulates development of colitis via alphavbeta1 integrin.
Authors: Kang S, Okuno T, Takegahara N, Takamatsu H, Nojima S, Kimura T, Yoshida Y, Ito D, Ohmae S, You DJ, Toyofuku T, Jang MH, Kumanogoh A
J. Immunol., 2011;188(3):1108-16.
Sample Types: Whole Cells
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