Human/Mouse Plexin C1 Antibody

Catalog # Availability Size / Price Qty
MAB3887
MAB3887-SP
Plexin C1 in Human Melanoma.
2 Images
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Human/Mouse Plexin C1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Plexin C1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Plexin A4, B1, B2, B3, D1, recombinant mouse Plexin A1, A2, or A3 is observed.
Source
Monoclonal Mouse IgG2B Clone # 544232
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Plexin C1
Ile49-Ser161
Accession # O60486
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry Plexin C1 in Human Melanoma. View Larger

Plexin C1 in Human Melanoma. Plexin C1 was detected in immersion fixed paraffin-embedded sections of human melanoma using Mouse Anti-Human Plexin C1 Monoclonal Antibody (Catalog # MAB3887) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Plexin C1 in Mouse Embryo. View Larger

Plexin C1 in Mouse Embryo. Plexin C1 was detected in immersion fixed frozen sections of mouse embryonic gut (12 d.p.c.) using Mouse Anti-Human Plexin C1 Monoclonal Antibody (Catalog # MAB3887) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin C1

Plexin C1 (also VESPR and CD232) is a 210 kDa member of the plexin-C subfamily, plexin family of proteins. It is a type I transmembrane glycoprotein that is found on monocytes, dendritic cells and neutrophils. Plexin C1 binds Sema7A, and its viral counterpart, vaccinia semaphorin A39R. Ligation induces actin cytoskeletal rearrangement and cell immobilization. Mature human Plexin C1 is 1534 amino acids (aa) in length. It contains a 910 aa extracellular domain (ECD) and a 603 aa cytoplasmic region. The ECD contains one Sema-domain (aa 49-440), two PSI domains (aa 454-605) and two IPT regions (aa 664-842). Over aa 49-161, human Plexin C1 shares 92% aa identity with mouse Plexin C1.

Entrez Gene IDs
10154 (Human); 54712 (Mouse); 362873 (Rat)
Alternate Names
CD232 antigen; CD232; plexin (semaphorin receptor); Plexin C1; PLXNC1; PLXN-C1; receptor for viral semaphorin protein; receptor for virally-encoded semaphorin; VESPR; VESPRplexin-C1; Virus-encoded semaphorin protein receptor

Product Datasheets

Citations for Human/Mouse Plexin C1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Viral semaphorin inhibits dendritic cell phagocytosis and migration but is not essential for gammaherpesvirus-induced lymphoproliferation in malignant catarrhal fever.
    Authors: Myster F, Palmeira L, Sorel O, Bouillenne F, DePauw E, Schwartz-Cornil I, Vanderplasschen A, Dewals B
    J Virol, 2015;89(7):3630-47.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Flow
  2. Intestinal epithelial cell-derived semaphorin 7A negatively regulates development of colitis via alphavbeta1 integrin.
    Authors: Kang S, Okuno T, Takegahara N, Takamatsu H, Nojima S, Kimura T, Yoshida Y, Ito D, Ohmae S, You DJ, Toyofuku T, Jang MH, Kumanogoh A
    J. Immunol., 2011;188(3):1108-16.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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