Human/Mouse/Rat Activin A beta A subunit Antibody

(9 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse and rat Activin A in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Activin A
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human/Mouse/Rat Activin A (Catalog # 338-AC)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize Activin A-induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. Schwall, R. H. et al. (1991) Method Enzymol. 198:340. The Neutralization Dose (ND50) is typically 2-6 µg/mL in the presence of 7.5 ng/mL Recombinant Human/Mouse/Rat Activin A.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Hemoglobin Expression Induced by Activin A and Neutralization by Human/Mouse/Rat Activin A Antibody. Recombinant Human/Mouse/Rat Activin A (Catalog # 338-AC) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin Expression elicited by Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Activin A beta A subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF338). The ND50 is typically 2‑6 µg/mL.
Immunohistochemistry
Activin A in Human Breast Cancer Tissue. Activin A was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 5 µg/mL Goat Anti-Human/Mouse/Rat Activin A beta A Subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF338) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Activin A
Activin and Inhibin are members of the TGF-beta superfamily of cytokines and are involved in a wide range of biological processes including tissue morphogenesis and repair, fibrosis, inflammation, neural development, hematopoiesis, reproductive system function, and carcinogenesis (1‑7). Activin and Inhibin are produced as precursor proteins. Their amino terminal propeptides are proteolytically cleaved and facilitate formation of disulfide-linked dimers of the bioactive proteins (8, 9). Activins are nonglycosylated homodimers or heterodimers of various beta subunits ( beta A, beta B, beta C, and beta E in mammals), while Inhibins are heterodimers of a unique alpha subunit and one of the beta subunits. Activin A is a widely expressed homodimer of two beta A chains. The beta A subunit can also heterodimerize with a beta B or beta C subunit to form Activin AB and Activin AC, respectively (10). The 14 kDa mature human beta A chain shares 100% amino acid sequence identity with bovine, feline, mouse, porcine, and rat beta A. Activin A exerts its biological activities by binding to the type 2 serine/threonine kinase Activin RIIA which then noncovalently associates with the type 1 serine/threonine kinase Activin RIB/ALK-4 (7, 11). Signaling through this receptor complex leads to Smad activation and regulation of activin-responsive gene transcription (7, 11). The bioactivity of Activin A is regulated by a variety of mechanisms (11). BAMBI, Betaglycan, and Cripto are cell-associated molecules that function as decoy receptors or limit the ability of Activin A to induce receptor complex assembly (12‑14). The intracellular formation of Activin A can be prevented by the incorporation of the beta A subunit into Activin AC or Inhibin A (3, 10). And the bioavailability of Activin A is restricted by its incorporation into inactive complexes with alpha 2-Macroglobulin, Follistatin, and FLRG (15, 16).
  • References:
    1. Kumanov, P. et al. (2005) Reprod. Biomed. Online 10:786.
    2. Maeshima, A. et al. (2008) Endocr. J. 55:1.
    3. Rodgarkia-Dara, C. et al. (2006) Mutat. Res. 613:123.
    4. Werner, S. and C. Alzheimer (2006) Cytokine Growth Factor Rev. 17:157.
    5. Xu, P. and A.K. Hall (2006) Dev. Biol. 299:303.
    6. Shav-Tal, Y. and D. Zipori (2002) Stem Cells 20:493.
    7. Chen, Y.G. et al. (2006) Exp. Biol. Med. 231:534.
    8. Gray, A.M. and A.J. Mason (1990) Science 247:1328.
    9. Mason, A.J. et al. (1996) Mol. Endocrinol. 10:1055.
    10. Thompson, T.B. et al. (2004) Mol. Cell. Endocrinol. 225:9.
    11. Harrison, C.A. et al. (2005) Trends Endocrinol. Metab. 16:73.
    12. Onichtchouk, D. et al. (1999) Nature 401:480.
    13. Gray, P.C. et al. (2002) Mol. Cell. Endocrinol. 188:254.
    14. Kelber, J.A. et al. (2008) J. Biol. Chem. 283:4490.
    15. Phillips, D.J. et al. (1997) J. Endocrinol. 155:65.
    16. Schneyer, A. et al. (2003) Endocrinology 144:1671.
  • Entrez Gene IDs:
    3624 (Human); 16323 (Mouse)
  • Alternate Names:
    Activin A; INHBA
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
Filter your results:

Species
Applications
Sample Type
  1. Hypoxia-induced activin A diminishes endothelial cell vasculogenic activity
    Authors: S Merfeld-Cl, H Lu, X Wu, KL March, DO Traktuev
    J. Cell. Mol. Med., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Differential Expression and Release of Activin A and Follistatin in Chronic Rhinosinusitis with and without Nasal Polyps.
    Authors: Yang Y, Zhang N, Crombruggen K, Lan F, Hu G, Hong S, Bachert C
    PLoS ONE, 2015;10(6):e0128564.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  3. Activin A as a mediator of NK-dendritic cell functional interactions.
    Authors: Seeger P, Bosisio D, Parolini S, Badolato R, Gismondi A, Santoni A, Sozzani S
    J Immunol, 2014;192(3):1241-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. Activin A induces growth arrest through a SMAD- dependent pathway in hepatic progenitor cells.
    Authors: Chen L, Zhang W, Liang H, Zhou Q, Ding Z, Yang H, Liu W, Wu Y, Man Q, Zhang B, Chen X
    Cell Commun Signal, 2014;12(0):18.
    Species: Rat
    Sample Type: Whole Cells
    Application: IHC Paraffin-embedded
  5. An activin receptor IIA ligand trap corrects ineffective erythropoiesis in beta-thalassemia.
    Authors: Dussiot M, Maciel T, Fricot A, Chartier C, Negre O, Veiga J, Grapton D, Paubelle E, Payen E, Beuzard Y, Leboulch P, Ribeil J, Arlet J, Cote F, Courtois G, Ginzburg Y, Daniel T, Chopra R, Sung V, Hermine O, Moura I
    Nat Med, 2014;20(4):398-407.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
  6. Inhibition of activin A ameliorates skeletal muscle injury and rescues contractile properties by inducing efficient remodeling in female mice.
    Authors: Yaden B, Wang Y, Wilson J, Culver A, Milner A, Datta-Mannan A, Shetler P, Croy J, Dai G, Krishnan V
    Am J Pathol, 2014;184(4):1152-66.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  7. Fgf-2 overexpression increases excitability and seizure susceptibility but decreases seizure-induced cell loss.
    Authors: Zucchini S, Buzzi A, Barbieri M, Rodi D, Paradiso B, Binaschi A, Coffin JD, Marzola A, Cifelli P, Belluzzi O, Simonato M
    J. Neurosci., 2008;28(49):13112-24.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. Interleukin-1 in combination with oncostatin M up-regulates multiple genes in chondrocytes: implications for cartilage destruction and repair.
    Authors: Barksby HE, Hui W, Wappler I, Peters HH, Milner JM, Richards CD, Cawston TE, Rowan AD
    Arthritis Rheum., 2006;54(2):540-50.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  9. Activin A is a potent activator of renal interstitial fibroblasts.
    Authors: Yamashita S, Maeshima A, Kojima I, Nojima Y
    J. Am. Soc. Nephrol., 2004;15(1):91-101.
    Species: Canine
    Sample Type: Whole Cells
    Application: ICC
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Basic

CTS013 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mounting Medium

CTS011 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Acidic

CTS014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Guard Mounting Media

NL996
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG HRP-conjugated Antibody

WB HAF017 15  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Antibody

WB AF109 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG VisUCyte HRP Polymer

IHC VC004  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human/Mouse/Rat Activin A beta A subunit Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information