Human/Mouse/Rat Akt2 Antibody

Catalog # Availability Size / Price Qty
MAB2315
MAB2315-SP
Detection of Human/Mouse/Rat Akt2 by Western Blot.
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Human/Mouse/Rat Akt2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Akt2. Detects human Akt2 in direct ELISAs and endogenous Akt2 in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Akt1 or rhAkt3 is observed.
Source
Monoclonal Mouse IgG3 Clone # 302514
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Akt2
Asn2-Glu481
Accession # P31751
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat Akt2 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat Akt2 by Western Blot. Western blot shows lysates of MDA-MB-468 human breast cancer cell line and Huh-7 human hepatoma cell line. PVDF membrane was probed with 2 µg/mL Mouse Anti-Human/Mouse/Rat Akt2 Monoclonal Antibody (Catalog # MAB2315) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Active Akt1 (Catalog # 1775-KS), recombinant human Akt2, and recombinant human Akt3 (2 ng/lane) were included. A specific band for Akt2 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Akt2

The serine/threonine kinase Akt, also known as protein kinase B (PKB), is a central regulator of such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. In mammals, three highly homologous members define the Akt family: Akt1 (PKB alpha ), Akt2 (PKB beta ), and Akt3 (PKB gamma ). Akt2 is expressed predominantly in insulin target tissues such as liver, skeletal muscle, and fat.

Long Name
v-Akt Murine Thymoma Viral Oncogene Homolog 2
Entrez Gene IDs
208 (Human); 11652 (Mouse); 29414 (Rat)
Alternate Names
Akt2; EC 2.7.11; EC 2.7.11.1; Murine thymoma viral (v-akt) homolog-2; PKB beta; PKBB; PKBBETA; PRKBB; Protein kinase Akt-2; Protein kinase B beta; rac protein kinase beta; RAC-beta serine/threonine-protein kinase; RAC-beta; RAC-PK-beta; v-akt murine thymoma viral oncogene homolog 2

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