Detection of Human/Mouse/Rat BAK by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line, CH‑1 mouse B cell lymphoma cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat BAK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8161) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BAK at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
BAK in HEK293 Human Cell Line.
BAK was detected in immersion fixed HEK293 human embryonic kidney cell line using Goat Anti-Human/Mouse/Rat BAK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8161) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
BAK (Bcl-2 homologous antagonist/killer; also BAK1) is a 25-30 kDa member of the BCL-2 family of proteins. It is widely expressed, and participates in the apoptotic cycle. BAK is an outer mitochondrial membrane protein that is inactive as a Zn-dependent homodimer. Upon activation by p53 or tBID, BAK oligomerizes, creating a pore in the mitochondrial membrane and allowing for cytochrome C release. Human BAK is 211 amino acids (aa) in length and contains three BCL-2 homology domains (aa 74-88, 117 -136 and 169-184), a Zn-binding region (aa 160-166) and a C-terminal transmembrane segment (aa 188-205). Amino acids 67-94 mediate oligomerization of BAK. There are two potential isoform variants; one shows an alternate start site at Met96, while a second shows a deletion of aa 46-66. Over amino acids 20-124, human BAK shares 76% aa identity with mouse BAK.
Entrez Gene IDs:
578 (Human); 12018 (Mouse)
Apoptosis regulator BAK; BAK; BAK1; BAK-LIKE; BAKMGC3887; BCL2-antagonist/killer 1; BCL2L7; bcl2-L-7; BCL2L7bcl-2 homologous antagonist/killer; BCL2-like 7 protein; Bcl-2-like protein 7; CDN1; MGC117255; pro-apoptotic protein BAK
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