hh Human/Mouse/Rat/Chicken Pax7 Antibody MAB1675: R&D Systems

Human/Mouse/Rat/Chicken Pax7 Antibody

(16 citations)   
  • Species Reactivity
    Human, Mouse, Rat, Chicken
  • Specificity
    Detects human, mouse, rat, and chicken Pax7 (3-8).
  • Source
    Monoclonal Mouse IgG1 Clone # PAX7
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant chicken Pax7
    aa 352-523
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Pax7 in C2C12 Mouse Cell Line. Pax7 was detected in immersion fixed C2C12 mouse myoblast cell line using 10 µg/mL Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) for 3 hours at room temperature. Cells were stained (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
Pax7 in iBJ6 iPS Cell Line. Pax7 was detected in immersion fixed iBJ6 iPS cell line differentiated into myoblasts using Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pax7
Pax7 belongs to the paired box protein family of transcription factors. It is an important regulator of neural and skeletal muscle development (1 - 3).
  • References:
    1. Jostes, B. et al. (1990) Mech. Dev. 33(1):27.
    2. Asakura, A. et al. (2001) Differentiation 68(4-5):245.
    3. Kawakami, A. et al. (1997) Mech. Dev. 66:119.
    4. Ericson, J. et al. (1996) Cell 87:661.
    5. Berggren, K. et al. (2001) Dev. Dyn. 222:1.
    6. Takebayashi, H. et al. (2002) Mech. Dev. 113:169.
    7. McLoon, L.K. and J. Wirtschafter (2003) Invest. Opthamal. Vis. Sci. 44:1927.
    8. Yamamoto, S. et al. (2001) J. Neurosci. 21:9814.
  • Long Name:
    Paired Box Gene 7
  • Entrez Gene IDs:
    5081 (Human); 18509 (Mouse); 500574 (Rat)
  • Alternate Names:
    FLJ37460; HUP1; paired box 7; paired box gene 7; paired box homeotic gene 7; paired box protein Pax-7; paired domain gene 7; PAX7 transcriptional factor; Pax7; PAX7B; RMS2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Irisin prevents and restores bone loss and muscle atrophy in hind-limb suspended mice
    Authors: G Colaianni, T Mongelli, C Cuscito, P Pignataro, L Lippo, G Spiro, A Notarnicol, I Severi, G Passeri, G Mori, G Brunetti, B Moretti, U Tarantino, SC Colucci, JE Reseland, R Vettor, S Cinti, M Grano
    Sci Rep, 2017;7(1):2811.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Deletion of the Ste20-like kinase SLK in skeletal muscle results in a progressive myopathy and muscle weakness
    Authors: BR Pryce, KN Al-Zahrani, S Dufresne, N Belkina, C Labr?che, G Patino-Lop, J Frenette, S Shaw, LA Sabourin
    Skelet Muscle, 2017;7(1):3.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: IHC
  3. CXCL12 and osteopontin from bone marrow-derived mesenchymal stromal cells improve muscle regeneration
    Authors: Y Maeda, Y Yonemochi, Y Nakajyo, H Hidaka, T Ikeda, Y Ando
    Sci Rep, 2017;7(1):3305.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. RAGE signaling deficiency in rhabdomyosarcoma cells causes upregulation of PAX7 and uncontrolled proliferation.
    Authors: Riuzzi, Francesc, Sorci, Guglielm, Sagheddu, Roberta, Sidoni, Angelo, Alaggio, Rita, Ninfo, Vito, Donato, Rosario
    J Cell Sci, 2014;127(0):1699-711.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Viral expression of insulin-like growth factor I E-peptides increases skeletal muscle mass but at the expense of strength.
    Authors: Brisson, Becky K, Spinazzola, Janelle, Park, SooHyun, Barton, Elisabet
    Am J Physiol Endocrinol Metab, 2014;306(8):E965-74.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC
  6. Regenerative defect in vastus lateralis muscle of patients with chronic obstructive pulmonary disease.
    Authors: Theriault M, Pare M, Lemire B, Maltais F, Debigare R
    Respir Res, 2014;15(0):35.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  7. Identification of FHL1 as a therapeutic target for Duchenne muscular dystrophy.
    Authors: D'Arcy, Colleen, Feeney, Sandra J, McLean, Catriona, Gehrig, Stefan M, Lynch, Gordon S, Smith, Jaclyn E, Cowling, Belinda, Mitchell, Christin, McGrath, Meagan J
    Hum Mol Genet, 2014;23(3):618-36.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  8. A simplified but robust method for the isolation of avian and mammalian muscle satellite cells.
    BMC Cell Biol., 2012;13(0):16.
    Species: Avian - Chicken
    Sample Type: Whole Cells
    Application: ICC
  9. Skeletal muscle regeneration in Xenopus tadpoles and zebrafish larvae.
    Authors: Rodrigues AM, Christen B, Marti M, Izpisua Belmonte JC
    BMC Dev. Biol., 2012;12(0):9.
    Species: Xenopus
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  10. Satellite cells senescence in limb muscle of severe patients with COPD.
    Authors: Theriault ME, Pare MÈ, Maltais F, Debigare R
    PLoS ONE, 2012;7(6):e39124.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  11. Selective development of myogenic mesenchymal cells from human embryonic and induced pluripotent stem cells.
    Authors: Awaya, Tomonari, Kato, Takeo, Mizuno, Yuta, Chang, Hsi, Niwa, Akira, Umeda, Katsutsu, Nakahata, Tatsutos, Heike, Toshio
    PLoS ONE, 2012;7(12):e51638.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  12. Sparing of extraocular muscle in aging and muscular dystrophies: a myogenic precursor cell hypothesis.
    Authors: Kallestad KM, Hebert SL, McDonald AA, Daniel ML, Cu SR, McLoon LK
    Exp. Cell Res., 2011;317(6):873-85.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  13. Extracellular matrix components direct porcine muscle stem cell behavior.
    Authors: Wilschut KJ, Haagsman HP, Roelen BA
    Exp. Cell Res., 2010;316(3):341-52.
    Species: Porcine
    Sample Type: Whole Cells
    Application: ICC
  14. Acceleration of skeletal muscle regeneration in a rat skeletal muscle injury model by local injection of human peripheral blood-derived CD133-positive cells.
    Authors: Shi M, Ishikawa M, Kamei N, Nakasa T, Adachi N, Deie M, Asahara T, Ochi M
    Stem Cells, 2009;27(4):949-60.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  15. Efficient conversion of ES cells into myogenic lineage using the gene-inducible system.
    Authors: Ozasa S, Kimura S, Ito K, Ueno H, Ikezawa M, Matsukura M, Yoshioka K, Araki K, Yamamura KI, Abe K, Niwa H, Miike T
    Biochem. Biophys. Res. Commun., 2007;357(4):957-63.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  16. Skeletal myogenesis by human embryonic stem cells.
    Authors: Zheng JK, Wang Y, Karandikar A, Wang Q, Gai H, Liu AL, Peng C, Sheng HZ
    Cell Res., 2006;16(8):713-22.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 16 Citations
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