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Human/Mouse/Rat/Chicken Pax7 Antibody

Catalog #: MAB1675
30 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1675
MAB1675-SP
Pax7 in C2C12 Mouse Cell Line.
3 Images
Product Details
Citations (30)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat/Chicken Pax7 Antibody Summary

Species Reactivity
Human, Mouse, Rat, Chicken
Specificity
Detects human, mouse, rat, and chicken Pax7 (3-8).
Source
Monoclonal Mouse IgG1 Clone # PAX7
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant chicken Pax7
aa 352-523
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
Immersion fixed paraffin-embedded sections of human skeletal muscle
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Pax7 antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Pax7 in C2C12 Mouse Cell Line. Pax7 was detected in immersion fixed C2C12 mouse myoblast cell line using 10 µg/mL Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) for 3 hours at room temperature. Cells were stained (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry Pax7 antibody in iBJ6 iPS Cell Line by Immunocytochemistry (ICC). View Larger

Pax7 in iBJ6 iPS Cell Line. Pax7 was detected in immersion fixed iBJ6 iPS cell line differentiated into myoblasts using Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunohistochemistry View Larger

Pax7 in Human Skeletal Muscle. Pax7 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pax7

Pax7 belongs to the paired box protein family of transcription factors. It is an important regulator of neural and skeletal muscle development (1 - 3).

References
  1. Jostes, B. et al. (1990) Mech. Dev. 33(1):27.
  2. Asakura, A. et al. (2001) Differentiation 68(4-5):245.
  3. Kawakami, A. et al. (1997) Mech. Dev. 66:119.
  4. Ericson, J. et al. (1996) Cell 87:661.
  5. Berggren, K. et al. (2001) Dev. Dyn. 222:1.
  6. Takebayashi, H. et al. (2002) Mech. Dev. 113:169.
  7. McLoon, L.K. and J. Wirtschafter (2003) Invest. Opthamal. Vis. Sci. 44:1927.
  8. Yamamoto, S. et al. (2001) J. Neurosci. 21:9814.
Long Name
Paired Box Gene 7
Entrez Gene IDs
5081 (Human); 18509 (Mouse); 500574 (Rat)
Alternate Names
FLJ37460; HUP1; paired box 7; paired box gene 7; paired box homeotic gene 7; paired box protein Pax-7; paired domain gene 7; PAX7 transcriptional factor; Pax7; PAX7B; RMS2

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Citations for Human/Mouse/Rat/Chicken Pax7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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  1. Replication collisions induced by de-repressed S-phase transcription are connected with malignant transformation of adult stem cells
    Authors: T Zhang, C Künne, D Ding, S Günther, X Guo, Y Zhou, X Yuan, T Braun
    Nature Communications, 2022;13(1):6907.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Integrative molecular roadmap for direct conversion of fibroblasts into myocytes and myogenic progenitor cells
    Authors: I Kim, A Ghosh, N Bundschuh, L Hinte, E Petrosyan, F von Meyenn, O Bar-Nur
    Science Advances, 2022;8(14):eabj4928.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC/IF
  3. Human spinal GABA neurons alleviate spasticity and improve locomotion in rats with spinal cord injury
    Authors: C Gong, X Zheng, F Guo, Y Wang, S Zhang, J Chen, X Sun, SZA Shah, Y Zheng, X Li, Y Yin, Q Li, X Huang, T Guo, X Han, SC Zhang, W Wang, H Chen
    Cell Reports, 2021;34(12):108889.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  4. VEGF and bFGF induction by nitric oxide is associated with hyperbaric oxygen-induced angiogenesis and muscle regeneration
    Authors: N Yamamoto, T Oyaizu, M Enomoto, M Horie, M Yuasa, A Okawa, K Yagishita
    Sci Rep, 2020;10(1):2744.
    Species: Mouse
    Sample Types: Whole Tissh
    Applications: IHC
  5. The natural involution of the sheep proximal sesamoidean ligament is due to depletion of satellite cells and simultaneous proliferation of fibroblasts: Ultrastructural evidence
    Authors: L Melotti, E Vezzoli, F Mascarello, L Maccatrozz, M Patruno
    Res. Vet. Sci., 2019;124(0):106-111.
    Species: Ovine
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. Inhibition of FLT1 ameliorates muscular dystrophy phenotype by increased vasculature in a mouse model of Duchenne muscular dystrophy
    Authors: M Verma, Y Shimizu-Mo, Y Asakura, JP Ennen, J Bosco, Z Zhou, GH Fong, S Josiah, D Keefe, A Asakura
    PLoS Genet., 2019;15(12):e1008468.
    Species: Mouse
    Sample Types: Tissue
    Applications: IHC frozen fixed
  7. Adipose tissue protects against sepsis-induced muscle weakness in mice: from lipolysis to ketones
    Authors: C Goossens, R Weckx, S Derde, T Dufour, S Vander Per, L Pauwels, SE Thiessen, PP Van Veldho, G Van den Be, L Langouche
    Crit Care, 2019;23(1):236.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Tbx1 regulates inherited metabolic and myogenic abilities of progenitor cells derived from slow- and fast-type muscle
    Authors: N Motohashi, A Uezumi, A Asakura, M Ikemoto-Ue, S Mori, Y Mizunoe, R Takashima, Y Miyagoe-Su, S Takeda, K Shigemoto
    Cell Death Differ., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Effect of 8-week leucine supplementation and resistance exercise training on muscle hypertrophy and satellite cell activation in rats
    Authors: CH Lim, JH Gil, H Quan, DH Viet, CK Kim
    Physiol Rep, 2018;6(12):e13725.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  10. Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line
    Authors: Y Kitajima, Y Ono
    Skelet Muscle, 2018;8(1):26.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Deletion of the Ste20-like kinase SLK in skeletal muscle results in a progressive myopathy and muscle weakness
    Authors: BR Pryce, KN Al-Zahrani, S Dufresne, N Belkina, C Labr?che, G Patino-Lop, J Frenette, S Shaw, LA Sabourin
    Skelet Muscle, 2017;7(1):3.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: IHC
  12. Generation of thalamic neurons from mouse embryonic stem cells
    Authors: A Shiraishi, K Muguruma, Y Sasai
    Development, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Recovery from impaired muscle growth arises from prolonged postnatal accretion of myonuclei in Atrx mutant mice
    Authors: MS Huh, KG Young, K Yan, T Price-O'De, DJ Picketts
    PLoS ONE, 2017;12(11):e0186989.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  14. CXCL12 and osteopontin from bone marrow-derived mesenchymal stromal cells improve muscle regeneration
    Authors: Y Maeda, Y Yonemochi, Y Nakajyo, H Hidaka, T Ikeda, Y Ando
    Sci Rep, 2017;7(1):3305.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Irisin prevents and restores bone loss and muscle atrophy in hind-limb suspended mice
    Authors: G Colaianni, T Mongelli, C Cuscito, P Pignataro, L Lippo, G Spiro, A Notarnicol, I Severi, G Passeri, G Mori, G Brunetti, B Moretti, U Tarantino, SC Colucci, JE Reseland, R Vettor, S Cinti, M Grano
    Sci Rep, 2017;7(1):2811.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. A Gingiva-Derived Mesenchymal Stem Cell-Laden Porcine Small Intestinal Submucosa Extracellular Matrix Construct Promotes Myomucosal Regeneration of the Tongue
    Authors: Qilin Xu
    Tissue Eng Part A, 2016;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. PRENATAL MYONUCLEI PLAY A CRUCIAL ROLE FOR SKELETAL MUSCLE HYPERTROPHY IN RODENTS
    Authors: Fuminori Kawano
    Am. J. Physiol., Cell Physiol, 2016;0(0):ajpcell.00151.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  18. Viral expression of insulin-like growth factor I E-peptides increases skeletal muscle mass but at the expense of strength.
    Authors: Brisson, Becky K, Spinazzola, Janelle, Park, SooHyun, Barton, Elisabet
    Am J Physiol Endocrinol Metab, 2014;306(8):E965-74.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  19. Regenerative defect in vastus lateralis muscle of patients with chronic obstructive pulmonary disease.
    Authors: Theriault M, Pare M, Lemire B, Maltais F, Debigare R
    Respir Res, 2014;15(0):35.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  20. Identification of FHL1 as a therapeutic target for Duchenne muscular dystrophy.
    Authors: D'Arcy, Colleen, Feeney, Sandra J, McLean, Catriona, Gehrig, Stefan M, Lynch, Gordon S, Smith, Jaclyn E, Cowling, Belinda, Mitchell, Christin, McGrath, Meagan J
    Hum Mol Genet, 2014;23(3):618-36.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. RAGE signaling deficiency in rhabdomyosarcoma cells causes upregulation of PAX7 and uncontrolled proliferation.
    Authors: Riuzzi, Francesc, Sorci, Guglielm, Sagheddu, Roberta, Sidoni, Angelo, Alaggio, Rita, Ninfo, Vito, Donato, Rosario
    J Cell Sci, 2014;127(0):1699-711.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. A simplified but robust method for the isolation of avian and mammalian muscle satellite cells.
    BMC Cell Biol., 2012;13(0):16.
    Species: Avian - Chicken
    Sample Types: Whole Cells
    Applications: ICC
  23. Satellite cells senescence in limb muscle of severe patients with COPD.
    Authors: Theriault ME, Pare MÈ, Maltais F, Debigare R
    PLoS ONE, 2012;7(6):e39124.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  24. Skeletal muscle regeneration in Xenopus tadpoles and zebrafish larvae.
    Authors: Rodrigues AM, Christen B, Marti M, Izpisua Belmonte JC
    BMC Dev. Biol., 2012;12(0):9.
    Species: Xenopus
    Sample Types: Whole Tissue
    Applications: IHC-P
  25. Selective development of myogenic mesenchymal cells from human embryonic and induced pluripotent stem cells.
    Authors: Awaya, Tomonari, Kato, Takeo, Mizuno, Yuta, Chang, Hsi, Niwa, Akira, Umeda, Katsutsu, Nakahata, Tatsutos, Heike, Toshio
    PLoS ONE, 2012;7(12):e51638.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. Sparing of extraocular muscle in aging and muscular dystrophies: a myogenic precursor cell hypothesis.
    Authors: Kallestad KM, Hebert SL, McDonald AA, Daniel ML, Cu SR, McLoon LK
    Exp. Cell Res., 2011;317(6):873-85.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. Extracellular matrix components direct porcine muscle stem cell behavior.
    Authors: Wilschut KJ, Haagsman HP, Roelen BA
    Exp. Cell Res., 2010;316(3):341-52.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: ICC
  28. Acceleration of skeletal muscle regeneration in a rat skeletal muscle injury model by local injection of human peripheral blood-derived CD133-positive cells.
    Authors: Shi M, Ishikawa M, Kamei N, Nakasa T, Adachi N, Deie M, Asahara T, Ochi M
    Stem Cells, 2009;27(4):949-60.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  29. Efficient conversion of ES cells into myogenic lineage using the gene-inducible system.
    Authors: Ozasa S, Kimura S, Ito K, Ueno H, Ikezawa M, Matsukura M, Yoshioka K, Araki K, Yamamura KI, Abe K, Niwa H, Miike T
    Biochem. Biophys. Res. Commun., 2007;357(4):957-63.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  30. Skeletal myogenesis by human embryonic stem cells.
    Authors: Zheng JK, Wang Y, Karandikar A, Wang Q, Gai H, Liu AL, Peng C, Sheng HZ
    Cell Res., 2006;16(8):713-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human/Mouse/Rat/Chicken Pax7 Antibody

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Human/Mouse/Rat/Chicken Pax7 Antibody
By Anonymous on 07/03/2022
Application: ICC/IF Sample Tested: single muscle fiber Species: Mouse

Fixed with PFA.Block with BSA.1:500 primary ab.

Immunocytochemistry/Immunofluorescence Human/Mouse/Rat/Chicken Pax7 Antibody MAB1675
Human/Mouse/Rat/Chicken Pax7 Antibody
By Anonymous on 03/29/2022
Application: ICC/IF Sample Tested: human muscle precursor cells,Muscle precursor cells Species: Human
Immunocytochemistry/Immunofluorescence Human/Mouse/Rat/Chicken Pax7 Antibody MAB1675
Human/Mouse/Rat/Chicken Pax7 Antibody
By Anonymous on 12/04/2020
Application: ICC/IF Sample Tested: Primary myoblasts Species: Horse and Mouse
Immunocytochemistry/Immunofluorescence Human/Mouse/Rat/Chicken Pax7 Antibody MAB1675