Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Cited:

Human, Mouse, Rat, Porcine, Avian - Chicken, Avian - Duck, Fish - Danio rerio (Zebrafish), Frog - Xenopus (African Clawed Frog), Ovine

Applications

Validated:

Immunohistochemistry, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, IHC frozen fixed

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PAX7
View all Pax7 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant chicken Pax7
aa 352-523

Specificity

Detects human, mouse, rat, and chicken Pax7 (3-8).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat/Chicken Pax7 Antibody

Pax7 antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC).

Pax7 in C2C12 Mouse Cell Line.

Pax7 was detected in immersion fixed C2C12 mouse myoblast cell line using 10 µg/mL Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) for 3 hours at room temperature. Cells were stained (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Pax7 antibody in iBJ6 iPS Cell Line by Immunocytochemistry (ICC).

Pax7 in iBJ6 iPS Cell Line.

Pax7 was detected in immersion fixed iBJ6 iPS cell line differentiated into myoblasts using Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Pax7 in Human Skeletal Muscle.

Pax7 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Pax7 by Western Blot

Detection of Pax7 by Western Blot

Protein expression analyses during differentiation and ID1 expression analyses. a Protein expression profiles of PAX7, MYOD, and Desmin were compared between regular pCM (rpCM) cells and pCM cells overexpressing miR-146b (pCM-146b OE) after 4 days of myotube differentiation. The graph represents a density comparison of Western blotting results (n = 3; *p < 0.05). b Expression profiles of ID1 were compared between the rpCM cells and pCM-146b OE cells (n = 3; ***p < 0.001) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32471354), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat/Chicken Pax7 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed C2C12 mouse myoblast cell line and iBJ6 iPS cell line differentiated into myoblasts

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skeletal muscle

Reviewed Applications

Read 4 reviews rated 4.8 using MAB1675 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Pax7

Pax7 belongs to the paired box protein family of transcription factors. It is an important regulator of neural and skeletal muscle development (1 - 3).

References

  1. Jostes, B. et al. (1990) Mech. Dev. 33(1):27.
  2. Asakura, A. et al. (2001) Differentiation 68(4-5):245.
  3. Kawakami, A. et al. (1997) Mech. Dev. 66:119.
  4. Ericson, J. et al. (1996) Cell 87:661.
  5. Berggren, K. et al. (2001) Dev. Dyn. 222:1.
  6. Takebayashi, H. et al. (2002) Mech. Dev. 113:169.
  7. McLoon, L.K. and J. Wirtschafter (2003) Invest. Opthamal. Vis. Sci. 44:1927.
  8. Yamamoto, S. et al. (2001) J. Neurosci. 21:9814.

Long Name

Paired Box Gene 7

Alternate Names

HUP1, RMS2

Entrez Gene IDs

5081 (Human); 18509 (Mouse); 500574 (Rat)

Gene Symbol

PAX7

Additional Pax7 Products

Product Documents for Human/Mouse/Rat/Chicken Pax7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat/Chicken Pax7 Antibody

For research use only

Citations for Human/Mouse/Rat/Chicken Pax7 Antibody

Customer Reviews for Human/Mouse/Rat/Chicken Pax7 Antibody (4)

4.8 out of 5
4 Customer Ratings
5 Stars
75%
4 Stars
25%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human/Mouse/Rat/Chicken Pax7 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 4 of 4 reviews Showing All
Filter By:
Clear Filters X
  • Human/Mouse/Rat/Chicken Pax7 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: single muscle fiber
    Species: Mouse
    Verified Customer | Posted 07/03/2022
    Fixed with PFA.Block with BSA.1:500 primary ab.
    Human/Mouse/Rat/Chicken Pax7 Antibody MAB1675
  • Human/Mouse/Rat/Chicken Pax7 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: human muscle precursor cells and Muscle precursor cells
    Species: Human
    Verified Customer | Posted 03/29/2022
    Human/Mouse/Rat/Chicken Pax7 Antibody MAB1675
  • Human/Mouse/Rat/Chicken Pax7 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Primary myoblasts
    Species: Horse and Mouse
    Verified Customer | Posted 12/04/2020
    Human/Mouse/Rat/Chicken Pax7 Antibody MAB1675
  • Name: Zachary Patinkin
    Application: Simple Western
    Sample Tested: Human skeletal muscle satellite-derived cell lysate
    Species: Human
    Verified Customer | Posted 02/25/2015
    Human Skeletal muscle satellite derived cells at 0, 4, and 7 days of differentiation. Specific band at 49 kDa.
    Human/Mouse/Rat/Chicken Pax7 Antibody MAB1675

There are no reviews that match your criteria.

Showing  1 - 4 of 4 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Human/Mouse/Rat/Chicken Pax7 Antibody

Showing  1 - 1 of 1 FAQ Showing All

  • Q: Does MAB1675 recognize Bovine PAX7?

    A: MAB1675 binds to the C-terminal region of the Chicken PAX7 sequence, a region that is not present in the bovine PAX7 sequence. We would not expect MAB1675 to recognize Bovine PAX7 for this reason. 

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies