Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Dopa Decarboxylase/DDC Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dopa Decarboxylase/DDC
Met1-Glu480
Accession # P20711

Specificity

Detects human Dopa Decarboxylase/DDC in direct ELISAs and human, mouse and rat Dopa Decarboxylase/DDC in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Dopa Decarboxylase/DDC Antibody

Detection of Human, Mouse, and Rat Dopa Decarboxylase/DDC antibody by Western Blot.

Detection of Human, Mouse, and Rat Dopa Decarboxylase/DDC by Western Blot.

Western blot shows lysates of mouse kidney tissue, PC-12 rat adrenal pheochromocytoma cell line, and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3564) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Dopa Decarboxylase/DDC at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Dopa Decarboxylase/DDC antibody in IMR-32 Human Cell Line by Immunocytochemistry (ICC).

Dopa Decarboxylase/DDC in IMR‑32 Human Cell Line.

Dopa Decarboxylase/DDC was detected in immersion fixed IMR-32 human neuroblastoma cell line using Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3564) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Dopa Decarboxylase/DDC antibody in Human Brain by Immunohistochemistry (IHC-P).

Dopa Decarboxylase/DDC in Human Brain.

Dopa Decarboxylase/DDC was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3564) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Dopa Decarboxylase/DDC antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

Dopa Decarboxylase/DDC in Mouse Brain.

Dopa Decarboxylase/DDC was detected in perfusion fixed frozen sections of mouse brain using Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3564) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse Dopa Decarboxylase/DDC by Immunocytochemistry/Immunofluorescence

Detection of Mouse Dopa Decarboxylase/DDC by Immunocytochemistry/Immunofluorescence

DOPA decarboxylase is expressed non-uniformly in the CNS vasculature.(A–F) Coronal section of a P7 C57BL/6 brain stained for either GS lectin (green) to mark blood vessels or DOPA decarboxylase (DDC; magenta). Rightmost column shows merged images. Images are from the same brain section and correspond to cortex (A and B), striatum (C and D), and basal forebrain (E and F). DDC immunoreactivity is observed in blood vessels and in axon terminals, and in the latter it appears as distinct puncta in the cortex or more concentrated staining in the striatum. Yellow arrows in (C) indicate a large vein. Str, striatum; Ctx, cortex; AC, anterior commissure; Scale bar: 50 um. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30188322), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat Dopa Decarboxylase/DDC Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed IMR-32 human neuroblastoma cell line

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (substantia nigra) and perfusion fixed frozen sections of mouse brain

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Dopa Decarboxylase/DDC (Catalog # 3564-DC), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Mouse kidney tissue, PC‑12 rat adrenal pheochromocytoma cell line, and human kidney tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dopa Decarboxylase/DDC

Dopa decarboxylase (DDC), also known as aromatic amino acid decarboxylase, is a group II decarboxylase (1). The enzyme catalyzes the decarboxylation of aromatic L-amino acids to produce the corresponding amines. DDC produces the neurotransmitters dopamine and serotonin from L-Dopa and L-5-hydroxytryptophan, respectively. The inhibition of DDC could be used for the treatment of schizophrenia and Parkinson's disease (2).

References

  1. Sandmeier, E. et al. (1994) Eur. J. Biochem. 221:997.
  2. Bertoldi, M. et al. (1996) J. Biol. Chem. 271:23954.

Alternate Names

AADC, DDC

Entrez Gene IDs

1644 (Human)

Gene Symbol

DDC

UniProt

P20711

Additional Dopa Decarboxylase/DDC Products

Product Documents for Human/Mouse/Rat Dopa Decarboxylase/DDC Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat Dopa Decarboxylase/DDC Antibody

For research use only

Citations for Human/Mouse/Rat Dopa Decarboxylase/DDC Antibody

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Protocols

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