FABP1/L-FABP Antibody

Detection of Mouse and Rat FABP1/L‑FABP by Simple Western^TM.

Simple Western lane view shows lysates of H4-II-E-C3 rat hepatoma cell line and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L-FABP at approximately 16 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat FABP1/L-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1565) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of FABP1/L-FABP by Western Blot

SVIP KO significantly reduces the intracellular levels of L-FABP. (A) RT-qPCR assay depicting relative levels of L-FABP mRNA in SVIP KO cells. The data are representative of mean ± SD of three independent experiments. (**** p < 0.0001, using unpaired t-test). (B) Western blot image confirming a significant reduction in the L-FABP protein levels in the SVIP-KO cells compared to wildtype (WT) cells. beta -actin was used as a loading control. (C) Relative expression levels of the L-FABP and beta -actin proteins in the wildtype and SVIP KO cells. The data are representative of mean ± SD of three independent experiments. (*** p = 0.0006, using 2-way ANOVA, ns = not significant). Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/26/15/7465), licensed under a CC-BY license. Not internally tested by R&D Systems.