Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FABP1/L-FABP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat FABP1/L-FABP
Met1-Ile127
Accession # P02692

Specificity

Detects rat FABP1/L-FABP in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat FABP1/L‑FABP Antibody

Detection of Human, Mouse, and Rat FABP1/L-FABP antibody by Western Blot.

Detection of Human, Mouse, and Rat FABP1/L‑FABP by Western Blot.

Western blot shows lysates of H4-II-E-C3 rat hepatoma cell line, mouse liver tissue, human liver tissue, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/ Mouse/Rat FABP1/L-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1565) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP1/L-FABP at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Rat FABP1/L-FABP antibody by Western Blot.

Detection of Rat FABP1/L‑FABP by Western Blot.

Western blot shows lysate of rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/ Rat FABP1/L-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1565) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP1/ L-FABP at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FABP1 antibody in Rat Liver by Immunohistochemistry (IHC-Fr).

FABP1 in Rat Liver.

FABP1 was detected in perfusion fixed frozen sections of rat liver using Goat Anti-Human/Mouse/Rat FABP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1565) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse and Rat FABP1/L-FABP antibody by Simple WesternTM.

Detection of Mouse and Rat FABP1/L‑FABP by Simple WesternTM.

Simple Western lane view shows lysates of H4-II-E-C3 rat hepatoma cell line and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L-FABP at approximately 16 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat FABP1/L-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1565) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat FABP1/L‑FABP Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed rat liver cells

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat liver

Simple Western

10 µg/mL
Sample: H4‑II‑E‑C3 rat hepatoma cell line and mouse liver tissue

Western Blot

0.2-0.5 µg/mL
Sample: Rat liver tissue, H4‑II‑E‑C3 rat hepatoma cell line, mouse liver tissue, human liver tissue, and HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FABP1/L-FABP

FABP1, also known as liver FABP (L-FABP, Z-protein and squalene, and sterol-carrier protein [SCP]) is a member of the intracellular FABP family. It is highly expressed in the liver, intestine, kidney, and lung. FABP1 binds free fatty acids, their co-enzyme A derivatives, and may be involved in intracellular lipid transport.

Long Name

Fatty Acid-Binding Protein 1

Alternate Names

L-FABP, LFABP

Entrez Gene IDs

2168 (Human); 14080 (Mouse); 24360 (Rat)

Gene Symbol

FABP1

UniProt

P02692

Additional FABP1/L-FABP Products

Product Documents for Human/Mouse/Rat FABP1/L‑FABP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat FABP1/L‑FABP Antibody

For research use only

Related Research Areas

Early Endodermal Lineage Markers
Obesity

Citations for Human/Mouse/Rat FABP1/L‑FABP Antibody

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Protocols

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