Human/Mouse/Rat FABP1/L-FABP Antibody

Catalog # Availability Size / Price Qty
AF1565
AF1565-SP
Detection  of Human, Mouse, and Rat FABP1/L‑FABP by Western Blot.
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Citations (1)
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Human/Mouse/Rat FABP1/L-FABP Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects rat FABP1/L-FABP in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat FABP1/L-FABP
Met1-Ile127
Accession # P02692
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2-0.5 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
Immersion fixed rat liver cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human, Mouse, and Rat FABP1/L‑FABP by Western Blot. View Larger

Detection of Human, Mouse, and Rat FABP1/L‑FABP by Western Blot. Western blot shows lysates of H4‑II‑E‑C3 rat hepatoma cell line, mouse liver tissue, human liver tissue, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/
Mouse/Rat FABP1/L‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1565) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP1/L‑FABP at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection  of Rat FABP1/L‑FABP by Western Blot. View Larger

Detection of Rat FABP1/L‑FABP by Western Blot. Western blot shows lysate of rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/
Rat FABP1/L‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1565) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP1/
L‑FABP at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry FABP1 in Rat Liver. View Larger

FABP1 in Rat Liver. FABP1 was detected in perfusion fixed frozen sections of rat liver using Goat Anti-Human/Mouse/Rat FABP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1565) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western Detection  of Mouse and Rat FABP1/L‑FABP by Simple  WesternTM. View Larger

Detection of Mouse and Rat FABP1/L‑FABP by Simple WesternTM. Simple Western lane view shows lysates of H4‑II‑E‑C3 rat hepatoma cell line and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L‑FABP at approximately 16 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat FABP1/L‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1565) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP1/L-FABP

FABP1, also known as liver FABP (L-FABP, Z-protein and squalene, and sterol-carrier protein [SCP]) is a member of the intracellular FABP family. It is highly expressed in the liver, intestine, kidney, and lung. FABP1 binds free fatty acids, their co-enzyme A derivatives, and may be involved in intracellular lipid transport.

Long Name
Fatty Acid-Binding Protein 1
Entrez Gene IDs
2168 (Human); 14080 (Mouse); 24360 (Rat)
Alternate Names
FABP1; FABPL; fatty acid binding protein 1, liver; fatty acid-binding protein, liver; LFABP; L-FABP; L-FABPFatty acid-binding protein 1; Liver-type fatty acid-binding protein

Product Datasheets

Citation for Human/Mouse/Rat FABP1/L-FABP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Identification of biomarkers associated with the development of hepatocellular carcinoma in CuZn superoxide dismutase deficient mice.
    Authors: Elchuri S, Naeemuddin M, Sharpe O, Robinson WH, Huang TT
    Proteomics, 2007;7(12):2121-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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