FKBP38 Antibody (391505)

R&D Systems | Catalog # MAB3580

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 391505
View all FKBP38 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FKBP38
Met58-Gly382
Accession # Q14318

Specificity

Detects endogenous human, mouse and rat FKBP38 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for FKBP38 Antibody (391505)

Detection of Human/Mouse/Rat FKBP38 antibody by Western Blot.

Detection of Human/Mouse/Rat FKBP38 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat FKBP38 Monoclonal Antibody (Catalog # MAB3580) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FKBP38 at approximately 60 to 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for FKBP38 Antibody (391505)

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line

Reviewed Applications

Read 1 review rated 3 using MAB3580 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FKBP38

FK506 Binding Proteins (FKBPs) are intracellular receptors for the immuno-suppressive drug FK506. The FKBP/FK506 complex exerts its immunosuppressive effects by inhibiting calcineurin, a calcium- and calmodulin-dependent serine/threonine phosphatase that functions as a critical signaling molecule during T-cell activation. FKBP38, also known as FKBP8, is a 355 to 412 amino acid (aa) protein with an apparent molecular mass of ~60‑64 kDa in SDS-PAGE. FKBP38 binds to and inhibits calcineurin even in the absence of FK506, indicating that FKBP38 is a constitutively active inhibitor of calcineurin. Additionally, FKBP38 co‑immunoprecipitates with Bcl-2 and Bcl-xL, suggesting that FKBP38 may regulate apoptosis by anchoring Bcl‑2 and Bcl‑xL to mitochondrial membranes. Mouse FKBP38 shares 94% and 87% sequence identity with human and rat FKBP38, respectively.

Long Name

38 kDa FK506 Binding Protein

Alternate Names

FKBP8

Entrez Gene IDs

23770 (Human); 14232 (Mouse); 290652 (Rat)

Gene Symbol

FKBP8

UniProt

Q14318

Additional FKBP38 Products

Product Documents for FKBP38 Antibody (391505)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for FKBP38 Antibody (391505)

For research use only

Related Research Areas

Phosphatases and Regulators

Citations for FKBP38 Antibody (391505)

Customer Reviews for FKBP38 Antibody (391505) (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 21950602
    Species: Human
    Verified Customer | Posted 02/20/2015

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