Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GAD1/GAD67 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GAD1/GAD67
Ala2-Asn97
Accession # Q99259

Specificity

Detects human GAD1/GAD67 in direct ELISAs and detects human, mouse, and rat GAD1/GAD67 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat GAD1/GAD67 Antibody

Detection of Human, Mouse, and Rat GAD1/GAD67 antibody by Western Blot.

Detection of Human, Mouse, and Rat GAD1/GAD67 by Western Blot.

Western blot shows lysates of human brain (hypothalamus) tissue, mouse brain (total) tissue, and rat brain (total) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2086) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody HAF017). A specific band was detected for GAD1/GAD67 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GAD1/GAD67 antibody in Human Spinal Cord by Immunohistochemistry (IHC-P).

GAD1/GAD67 in Human Spinal Cord.

GAD1/GAD67 was detected in immersion fixed paraffin-embedded sections of human spinal cord using 15 µg/mL Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2086) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Mouse GAD1/GAD67 antibody by Simple WesternTM.

Detection of Mouse GAD1/GAD67 by Simple WesternTM.

Simple Western lane view shows lysates of mouse brain tissue, loaded at 0.2 mg/mL. A specific band was detected for GAD1/GAD67 at approximately 65 kDa (as indicated) using 1 µg/mL of Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2086) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat GAD1/GAD67 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spinal cord

Simple Western

1 µg/mL
Sample:   Mouse brain tissue

Western Blot

1 µg/mL
Sample:   Human brain (hypothalamus) tissue, mouse brain (total) tissue, and rat brain (total) tissue

Reviewed Applications

Read 1 review rated 1 using AF2086 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GAD1/GAD67

GAD1, also named 67 kDa or brain GAD, is an enzyme that catalyzes the formation of the inhibitory neurotransmitter gamma -amino butyric acid (GABA) from glutamate. GAD1 is also expressed in multiple non-neuronal tissues during development.

Long Name

Glutamate Decarboxylase 1 (Brain, 67kDa)

Alternate Names

GAD67

Entrez Gene IDs

2571 (Human)

Gene Symbol

GAD1

UniProt

Q99259

Additional GAD1/GAD67 Products

Product Documents for Human/Mouse/Rat GAD1/GAD67 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat GAD1/GAD67 Antibody

For research use only

Citations for Human/Mouse/Rat GAD1/GAD67 Antibody

Customer Reviews for Human/Mouse/Rat GAD1/GAD67 Antibody (1)

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  • Human/Mouse/Rat GAD1/GAD67 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult brain (hippocampus)
    Species: Mouse
    Verified Customer | Posted 04/28/2023
    We reached out to the customer offering to troubleshoot with no response. Does not work with incubation at 1:500 to 1:1000 dilutions overnight plus secondary AlexaFluor (multiple tested). No fluorescence detected. Sample tissue fixed with PFA.
    Human/Mouse/Rat GAD1/GAD67 Antibody AF2086
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

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Protocols

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FAQs

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