Human/Mouse/Rat GBL Antibody Summary
Accession # Q9BVC4
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human/Mouse/Rat G beta L by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat G beta L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4004) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for G beta L at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
G beta L in Human Prostate. G beta L was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human/Mouse/Rat G beta L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4004) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
G-protein beta -subunit-like (G beta L), also known as mLST8, is a subunit of the Target of Rapamycin (TOR) complex. G beta L contains 7 WD-40 repeats, and binds to the kinase domain of TOR in both the Raptor (Rapamycin-sensitive)-and Rictor (Rapamycin-insensitive)-containing complexes. G beta L is necessary for the full activation of the TOR kinase, and phosphorylation of many downstream proteins, including 4E-BP1 and p70S6 kinase.
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