Human/Mouse/Rat Histone H2AX Antibody

Catalog # Availability Size / Price Qty
MAB3406
MAB3406-SP
Detection of Human/Mouse/Rat Histone H2AX by Western Blot.
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Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (2)

Human/Mouse/Rat Histone H2AX Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat Histone H2AX in Western blots.
Source
Monoclonal Mouse IgG2A Clone # 322105
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Histone H2AX
Gly3-Ser131
Accession # P16104
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat Histone H2AX antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat Histone H2AX by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, K562 human chronic myelogenous leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Histone H2AX Monoclonal Antibody (Catalog # MAB3406) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Histone H2AX at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Histone H2AX antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Histone H2AX in HeLa Human Cell Line. Histone H2AX was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Histone H2AX Monoclonal Antibody (Catalog # MAB3406) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Histone H2AX

Histone H2AX is a core histone protein that is phosphorylated at S139 in cells exposed to DNA double-strand break-inducing agents, such as ionizing radiation. The S139 phosphorylated H2AX, termed gamma -H2AX, marks the site of DNA double-strand breaks and serves to recruit cell cycle checkpoint and DNA repair factors to the site of damage.

Entrez Gene IDs
3014 (Human)
Alternate Names
H2A.X; H2A/X; H2AFX; Histone H2AX

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Citations for Human/Mouse/Rat Histone H2AX Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Melflufen, a peptide-conjugated alkylator, is an efficient anti-neo-plastic drug in breast cancer cell lines
    Authors: A Schepsky, G Asta Traus, J Petur Joel, S Ingthorsso, J Kricker, J Thor Bergt, A Asbjarnars, T Gudjonsson, N Nupponen, A Slipicevic, F Lehmann, T Gudjonsson
    Cancer Med, 2020;0(0):.
    Species: Human
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  2. Phenotypic Plasticity of Invasive Edge Glioma Stem-like Cells in Response to Ionizing Radiation
    Authors: M Minata, A Audia, J Shi, S Lu, J Bernstock, MS Pavlyukov, A Das, SH Kim, YJ Shin, Y Lee, H Koo, K Snigdha, I Waghmare, X Guo, A Mohyeldin, D Gallego-Pe, J Wang, D Chen, P Cheng, F Mukheef, M Contreras, JF Reyes, B Vaillant, EP Sulman, SY Cheng, JM Markert, BA Tannous, X Lu, M Kango-Sing, LJ Lee, DH Nam, I Nakano, KP Bhat
    Cell Rep, 2019;26(7):1893-1905.e7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Platelet glycoprotein VI and C-type lectin-like receptor 2 deficiency accelerates wound healing by impairing vascular integrity in mice
    Authors: S Wichaiyo, S Lax, SJ Montague, Z Li, B Grygielska, JA Pike, EJ Haining, A Brill, SP Watson, J Rayes
    Haematologica, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Quantitative analysis of ATM phosphorylation in lymphocytes
    Authors: CJ Bakkenist, RK Czambel, Y Lin, NA Yates, X Zeng, J Shogan, JC Schmitz
    DNA Repair (Amst.), 2019;80(0):1-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: IR Fluorescence
  5. miR-24-mediated knockdown of H2AX damages mitochondria and the insulin signaling pathway
    Authors: JH Jeong, Y Cheol Kang, Y Piao, S Kang, YK Pak
    Exp. Mol. Med., 2017;49(4):e313.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. The formyl peptide receptor 1 exerts a tumor suppressor function in human gastric cancer by inhibiting angiogenesis.
    Authors: Prevete N, Liotti F, Visciano C, Marone G, Melillo R, de Paulis A
    Oncogene, 2015;34(29):3826-38.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse/Rat Histone H2AX Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human/Mouse/Rat Histone H2AX Antibody
By Anonymous on 11/04/2021
Application: ICC/IF Sample Tested: Splenocytes Species: Mouse

Human/Mouse/Rat Histone H2AX Antibody
By Anonymous on 01/24/2019
Application: WB Sample Tested: Brain (cerebral cortex) Species: Mouse

10 micrograms of mouse cortex
antibody used at 1/1300
1 second exposition
a: membrane after blotting with 15kDa molecular weight (*)
b : membrane after blotting with 20kDa molecular weight (**)