Human/Mouse/Rat MCHR1 Antibody

Catalog # Availability Size / Price Qty
MAB7938
MAB7938-SP
Detection of Human, Mouse, and Rat MCHR1 by Western Blot.
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Human/Mouse/Rat MCHR1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human MCHR1 in ELISAs. Detects human, mouse and rat MCHR1 in Western Blots
Source
Monoclonal Mouse IgG2A Clone # 851047
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human MCHR1
Ser2-Pro113
Accession # Q99705
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat MCHR1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat MCHR1 by Western Blot. Western blot shows lysates of human brain (hypothalamus) tissue, mouse brain (thalamus/hypothalamus) tissue, and rat brain (no cortex) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat MCHR1 Monoclonal Antibody (Catalog # MAB7938) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MCHR1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human and Mouse MCHR1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human and Mouse MCHR1 by Simple WesternTM. Simple Western lane view shows lysates of human brain (hypothalamus) tissue and mouse brain tissue, loaded at 0.5 mg/mL. A specific band was detected for MCHR1 at approximately 50-52 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human/Mouse/Rat MCHR1 Monoclonal Antibody (Catalog # MAB7938). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MCHR1

MCHR1 (melanin-concentrating hormone receptor-1), also called GPR24 (G-protein coupled receptor 24) or SLC1 (somatostatin receptor-like 1), is an approximately 44 kDa, seven-transmembrane receptor for MHC. It is most highly expressed in the brain but is also present in peripheral tissues. Engagement of MHCR1 has effects on food intake, energy homeostasis, anxiety and depression, such that rodents treated with small molecule antagonists weigh less and show reduced anxiety and depression in model systems than untreated rodents. Human MCHR1 shares 74% amino acid (aa) sequence identity with mouse MCHR1 between aa 1-113, and 82% with rat MCHR1 between aa 71-113.

Long Name
Melanin-concentrating Hormone Receptor 1
Entrez Gene IDs
2847 (Human); 207911 (Mouse); 83567 (Rat)
Alternate Names
G protein-coupled receptor 24; GPR24; GPR24SLC-1; G-protein coupled receptor 24; MCH receptor 1; MCH1R; MCH-1R; MCH1RMCHR-1; MCHR1; MCH-R1; melanin-concentrating hormone receptor 1; MGC32129; SLC1; SLC1MCHR; Somatostatin receptor-like protein

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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