MCHR1 Antibody (851047)

R&D Systems | Catalog # MAB7938

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 851047
View all MCHR1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MCHR1
Ser2-Pro113
Accession # Q99705

Specificity

Detects human MCHR1 in ELISAs. Detects human, mouse and rat MCHR1 in Western Blots

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for MCHR1 Antibody (851047)

Detection of Human, Mouse, and Rat MCHR1 antibody by Western Blot.

Detection of Human, Mouse, and Rat MCHR1 by Western Blot.

Western blot shows lysates of human brain (hypothalamus) tissue, mouse brain (thalamus/hypothalamus) tissue, and rat brain (no cortex) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat MCHR1 Monoclonal Antibody (Catalog # MAB7938) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MCHR1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human and Mouse MCHR1 antibody by Simple WesternTM.

Detection of Human and Mouse MCHR1 by Simple WesternTM.

Simple Western lane view shows lysates of human brain (hypothalamus) tissue and mouse brain tissue, loaded at 0.5 mg/mL. A specific band was detected for MCHR1 at approximately 50-52 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human/Mouse/Rat MCHR1 Monoclonal Antibody (Catalog # MAB7938). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for MCHR1 Antibody (851047)

Application
Recommended Usage

Simple Western

50 µg/mL
Sample: human brain (hypothalamus) tissue and mouse brain tissue

Western Blot

1 µg/mL
Sample: Human brain (hypothalamus) tissue, mouse brain (thalamus/hypothalamus) tissue, and rat brain (no cortex) tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MCHR1

MCHR1 (melanin-concentrating hormone receptor-1), also called GPR24 (G-protein coupled receptor 24) or SLC1 (somatostatin receptor-like 1), is an approximately 44 kDa, seven-transmembrane receptor for MHC. It is most highly expressed in the brain but is also present in peripheral tissues. Engagement of MHCR1 has effects on food intake, energy homeostasis, anxiety and depression, such that rodents treated with small molecule antagonists weigh less and show reduced anxiety and depression in model systems than untreated rodents. Human MCHR1 shares 74% amino acid (aa) sequence identity with mouse MCHR1 between aa 1-113, and 82% with rat MCHR1 between aa 71-113.

Long Name

Melanin-concentrating Hormone Receptor 1

Alternate Names

GPR24, MCH1R, SLC1

Entrez Gene IDs

2847 (Human); 207911 (Mouse); 83567 (Rat)

Gene Symbol

MCHR1

UniProt

Q99705 (Human)

Additional MCHR1 Products

Product Documents for MCHR1 Antibody (851047)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for MCHR1 Antibody (851047)

For research use only

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