Human/Mouse/Rat MEK1 Antibody

Name: Human/Mouse/Rat MEK1 Antibody
Brand: R&D Systems
SKU: AF2079
Price: 149 USD
Availability: InStock

R&D Systems | Catalog # AF2079

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all MEK1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MEK1
Lys2-Val393 (Ser218Asp, Ser222Asp)
Accession # Q02750

Specificity

Detects human, mouse, and rat MEK1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat MEK1 Antibody

Detection of Human/Mouse/Rat MEK1 by Western Blot.

Western blot shows lysates of BT-20 human breast cancer cell line, U937 human histiocytic lymphoma cell line, DU145 human prostate carcinoma cell line, and mouse brain tissue. PVDF membrane was probed with 0.2 µg/mL Goat Anti-Human/Mouse/Rat MEK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2079) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human MEK1 (5 ng) was included. A specific band for MEK1 was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat MEK1 Antibody

Application

Recommended Usage

Western Blot

0.2 µg/mL
Sample: BT-20 human breast cancer cell line, U937 human histiocytic lymphoma cell line, DU145 human prostate carcinoma cell line, and mouse brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: MEK1

MEK1 and MEK2, also known as MAP2K1 and MAP2K2, are activated in response to mitogenic stimuli. Activation occurs through dual phosphorylation at S218/S222 of MEK1 and S222/S226 of MEK2 by several upstream MAP3Ks. Both MEK1 and MEK2 are dual-specificity protein kinases, phosphorylating and activating ERK1 and ERK2 at Thr and Tyr positions.