Human/Mouse/Rat Musashi-1 Antibody

Catalog # Availability Size / Price Qty
AF2628
AF2628-SP
Detection  of Recombinant Human Musashi‑1 by Western Blot.
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Product Details
Citations (4)
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Human/Mouse/Rat Musashi-1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Musashi-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Musashi‑2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Musashi-1
Met1-His362
Accession # O43347
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunohistochemistry
1-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Recombinant Human Musashi‑1 by Western Blot. View Larger

Detection of Recombinant Human Musashi‑1 by Western Blot. Western blot shows 25 ng of Recombinant Human Musashi-1 and Recombinant Human Musashi-2. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/
Rat Musashi‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Musashi‑1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry Musashi‑1 in Mouse Cortical Stem Cells. View Larger

Musashi‑1 in Mouse Cortical Stem Cells. Musashi‑1 was detected in immersion fixed undifferentiated mouse cortical stem cells using Goat Anti-Human/Mouse/Rat
Musashi‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunocytochemistry Musashi‑1 in Rat Cortical Stem Cells. View Larger

Musashi‑1 in Rat Cortical Stem Cells. Musashi‑1 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Human/Mouse/Rat Musashi‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunohistochemistry Musashi‑1 in Human Intestine. View Larger

Musashi‑1 in Human Intestine. Musashi‑1 was detected in immersion fixed paraffin-embedded sections of human intestine using 1 µg/mL Goat Anti-Human/Mouse/
Rat Musashi‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) overnight at
4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Musashi-1

Musashi-1 is an RNA-binding protein that is expressed in the developing central nervous system. Expression is highest in proliferating multipotent neural precursor cells and decreases during neuronal differentiation (1, 2).

References
  1. Kaneko, Y. et al. (2000) Dev. Neurosci. 22:139.
  2. Kanemura, Y. et al. (2002) Meth. Mol. Biol. 198:273.
Entrez Gene IDs
4440 (Human)
Alternate Names
MSI1; Musashi (Drosophila) homolog 1; musashi homolog 1 (Drosophila); Musashi1; Musashi-1; RNA-binding protein Musashi homolog 1

Product Datasheets

Citations for Human/Mouse/Rat Musashi-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Musashi-1 is the candidate of the regulator of hair cell progenitors during inner ear regeneration
    Authors: T Wakasaki, H Niiro, S Jabbarzade, M Ohashi, T Kimitsuki, T Nakagawa, S Komune, K Akashi
    BMC Neurosci, 2017;18(1):64.
    Species: Chicken
    Sample Types: Whooe Tissue
    Applications: IHC OCT-embedded
  2. Rapid generation of OPC-like cells from human pluripotent stem cells for treating spinal cord injury
    Authors: DS Kim, SJ Jung, JS Lee, BY Lim, HA Kim, JE Yoo, DW Kim, JW Leem
    Exp. Mol. Med., 2017;49(7):e361.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Expression and regulation of prostate apoptosis response-4 (Par-4) in human glioma stem cells in drug-induced apoptosis.
    Authors: Jagtap, Jayashre, Dawood, Parveen, Shah, Reecha D, Chandrika, Goparaju, Natesh, Kumar, Shiras, Anjali, Hegde, Amba S, Ranade, Deepak, Shastry, Padma
    PLoS ONE, 2014;9(2):e88505.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Side populations of gastrointestinal cancers are not enriched in stem cells.
    Authors: Burkert J, Otto WR, Wright NA
    J. Pathol., 2008;214(5):564-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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