Human/Mouse/Rat Musashi-1 Antibody

(4 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human Musashi-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Musashi‑2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Musashi-1
    Met1-His362
    Accession # O43347
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Immunohistochemistry
    1-15 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Recombinant Human Musashi‑1 by Western Blot. Western blot shows 25 ng of Recombinant Human Musashi-1 and Recombinant Human Musashi-2. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/
Rat Musashi‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Musashi‑1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Immunocytochemistry
Musashi‑1 in Mouse Cortical Stem Cells. Musashi‑1 was detected in immersion fixed undifferentiated mouse cortical stem cells using Goat Anti-Human/Mouse/Rat
Musashi‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Immunocytochemistry
Musashi‑1 in Rat Cortical Stem Cells. Musashi‑1 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Human/Mouse/Rat Musashi‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Immunohistochemistry
Musashi‑1 in Human Intestine. Musashi‑1 was detected in immersion fixed paraffin-embedded sections of human intestine using 1 µg/mL Goat Anti-Human/Mouse/
Rat Musashi‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) overnight at
4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Musashi-1
Musashi-1 is an RNA-binding protein that is expressed in the developing central nervous system. Expression is highest in proliferating multipotent neural precursor cells and decreases during neuronal differentiation (1, 2).
  • References:
    1. Kaneko, Y. et al. (2000) Dev. Neurosci. 22:139.
    2. Kanemura, Y. et al. (2002) Meth. Mol. Biol. 198:273.
  • Entrez Gene IDs:
    4440 (Human)
  • Alternate Names:
    MSI1; Musashi (Drosophila) homolog 1; musashi homolog 1 (Drosophila); Musashi1; Musashi-1; RNA-binding protein Musashi homolog 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Rapid generation of OPC-like cells from human pluripotent stem cells for treating spinal cord injury
    Authors: DS Kim, SJ Jung, JS Lee, BY Lim, HA Kim, JE Yoo, DW Kim, JW Leem
    Exp. Mol. Med., 2017;49(7):e361.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. Musashi-1 is the candidate of the regulator of hair cell progenitors during inner ear regeneration
    Authors: T Wakasaki, H Niiro, S Jabbarzade, M Ohashi, T Kimitsuki, T Nakagawa, S Komune, K Akashi
    BMC Neurosci, 2017;18(1):64.
    Species: Chicken
    Sample Type: Whooe Tissue
    Application: IHC OCT-embedded
  3. Expression and regulation of prostate apoptosis response-4 (Par-4) in human glioma stem cells in drug-induced apoptosis.
    Authors: Jagtap, Jayashre, Dawood, Parveen, Shah, Reecha D, Chandrika, Goparaju, Natesh, Kumar, Shiras, Anjali, Hegde, Amba S, Ranade, Deepak, Shastry, Padma
    PLoS ONE, 2014;9(2):e88505.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. Side populations of gastrointestinal cancers are not enriched in stem cells.
    Authors: Burkert J, Otto WR, Wright NA
    J. Pathol., 2008;214(5):564-73.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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