Human/Mouse/Rat Neuropilin-2 Antibody

(11 citations)
(2 Reviews)
  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat Neuropilin-2 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Neuropilin‑2
    Gln23-Tyr855
    Accession # Q7LBX6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    HUVEC human umbilical vein endothelial cells
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 1-3 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of Recombinant Human VEGF165 (Catalog # 293-VE) to immobilized Recombinant Human Neuropilin-2 Fc Chimera (Catalog # 2215‑N2) coated at 5 µg/mL (100 µL/well). At 20 μg/mL, this antibody will block >90% of the binding.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Neuropilin‑2 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Neuropilin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2215) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Neuropilin‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Neuropilin‑2 in Human Pancreatic Cancer Tissue. Neuropilin‑2 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-Human/Mouse/Rat Neuropilin‑2 Antigen Affinity-purified Poly­clonal Antibody (Catalog # AF2215) at 5 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human, Mouse, and Rat Neuropilin‑2 by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, C6 rat glioma cell line, LL/2 mouse Lewis lung carcinoma cell line, and bEnd.3 mouse endothelioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Neuropilin‑2 at approximately 140 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Neuropilin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2215) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neuropilin-2

Neuropilin-2 (Npn-2) is a 120 kDa, type I transmembrane (TM) glycoprotein that is related to the semaphorin receptor now known as Neuropilin-1 (1). Npn-2 is a complex molecule with multiple splice forms. Five transmembrane forms are known, and one 62 kDa soluble form has been identified (2). Based on the originally reported precursor size of 909 amino acids (aa), the “standard” precursor in human will have a 20 aa signal sequence, an 842 aa extracellular region, a 25 aa TM segment, and a 42 aa cytoplasmic tail (1). The extracellular region contains two N-terminal CUB (C1r/Ugef/BMP-1) domains, two jellyroll-shaped coagulation factor V type C domains, and a juxtamembrane MAM (meprin/A-5 protein/tyrosine phosphatase μ) domain (1, 3). The CUB and factor V domain are involved in VEGF and semaphorin binding. The MAM domain appears necessary for signaling through plexin-1 (4). The five transmembrane isoforms all share the same CUB, factor V and MAM domains. Splicing begins at aa 809, seven amino acids after the end of the MAM domain, and it involves the end of the extracellular region, the TM segment, and the cytoplasmic domain (a total of 101 aa). Two of the four variants show a complete replacement of these 101 aa with a totally unrelated stretch of approximately 90 aa. This creates a new TM and cytoplasmic tail. These forms are called “Npn-2b” forms. Two other isoforms (plus the standard 909 aa form) retain the 101 aa stretch, and add either 17 or 22 aa to the end of the extracellular region. These forms are called “Npn-2a” forms. The isoform offered by R&D Systems is the “a” form with the 17 aa addition. This isoform shows 94% aa identity to the equivalent regions in mouse and rat Npn-2. The soluble form of Npn-2 is 555 aa in precursor length, and contains the two CUB domains plus the first 1½ factor V type C domains (1). Npn-2 binds Sema3B through F, and VEGF isoforms 165, 145, PlGF-2, and VEGF-C (5). It is known to form homodimers and heterodimers with Npn-1, and it forms receptor complexes with plexin-1 and VEGF R1 (4, 5). Npn-2 is found on a variety of cell types including neurons (motor, autonomic, sensory), vascular endothelial cells, Schwann cells and pancreatic acinar cells.

  • References:
    1. Chen, H. et al. (1997) Neuron 19:547.
    2. Rossignol, M. et al. (2000) Genomics 70:211.
    3. He, Z. and M. Tessier-lavigne (1997) Cell 90:739.
    4. Nakamura, F. and Y. Goshima (2002) Adv. Exp. Med. Biol. 515:55.
    5. Neufeld, G. et al. (2002) Adv. Exp. Med. Biol. 515:81.
  • Entrez Gene IDs:
    8828 (Human); 18187 (Mouse); 81527 (Rat)
  • Alternate Names:
    MGC126574; neuropilin 2; Neuropilin2; Neuropilin-2; neuropilin-2a(22); neuropilin-2b(0); NP2; NPN 2; NPN2; PRO2714; receptor for VEGF165 and semaphorins class3; Vascular endothelial cell growth factor 165 receptor 2; vascular endothelial growth factor-165 receptor 2; VEGF1265R2; VEGF165R2neuropilin-2a(17)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4
    Authors: LD Volk-Drape, KL Hall, AC Wilber, S Ran
    PLoS ONE, 2017;12(6):e0179257.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. Promotion of metastasis of thyroid cancer cells via NRP-2-mediated induction
    Oncol Lett, 2016;12(5):4224-4230.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Class 3 semaphorins induce F-actin reorganization in human dendritic cells: Role in cell migration
    J Leukoc Biol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Neuropilin-2 Regulates Endosome Maturation and EGFR Trafficking to Support Cancer Cell Pathobiology.
    Authors: Dutta S, Roy S, Polavaram N, Stanton M, Zhang H, Bhola T, Honscheid P, Donohue T, Band H, Batra S, Muders M, Datta K
    Cancer Res, 2016;76(2):418-28.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Neuropilin 1 is an entry factor that promotes EBV infection of nasopharyngeal epithelial cells.
    Authors: Wang, Hong-Bo, Zhang, Hua, Zhang, Jing-Pin, Li, Yan, Zhao, Bo, Feng, Guo-Kai, Du, Yong, Xiong, Dan, Zhong, Qian, Liu, Wan-Li, Du, Huamao, Li, Man-Zhi, Huang, Wen-Lin, Tsao, Sai Wah, Hutt-Fletcher, Lindsey, Zeng, Yi-Xin, Kieff, Elliott, Zeng, Mu-Sheng
    Nat Commun, 2015;6(0):6240.
    Species: Human
    Sample Type: Whole Cells
    Application: Blocking
  6. Bone marrow-derived cells are implicated as a source of lymphatic endothelial progenitors in human breast cancer.
    Authors: Van't Hull E, Bron S, Henry L, Ifticene-Treboux A, Turrini R, Coukos G, Delaloye J, Doucey M
    Oncoimmunology, 2014;3(0):e29080.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  7. Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes.
    Authors: Boyer-Di Ponio J, El-Ayoubi F, Glacial F, Ganeshamoorthy K, Driancourt C, Godet M, Perriere N, Guillevic O, Couraud P, Uzan G
    PLoS ONE, 2014;9(1):e84179.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  8. A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy.
    Authors: Hagerling R, Pollmann C, Andreas M, Schmidt C, Nurmi H, Adams R, Alitalo K, Andresen V, Schulte-Merker S, Kiefer F
    EMBO J, 2013;32(5):629-44.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC OCT-embedded
  9. Neuropilin-2 Is upregulated in lung cancer cells during TGF-beta1-induced epithelial-mesenchymal transition.
    Authors: Nasarre P, Gemmill R, Potiron V, Roche J, Lu X, Baron A, Korch C, Garrett-Mayer E, Lagana A, Howe P, Drabkin H
    Cancer Res, 2013;73(23):7111-21.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  10. Sequential arrival and graded secretion of Sema3F by olfactory neuron axons specify map topography at the bulb.
    Authors: Takeuchi H, Inokuchi K, Aoki M
    Cell, 2010;141(6):1056-67.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  11. Soluble neuropilin-2, a nerve repellent receptor, is increased in rheumatoid arthritis synovium and aggravates sympathetic fiber repulsion and arthritis.
    Authors: Fassold A, Falk W, Anders S, Hirsch T, Mirsky VM, Straub RH
    Arthritis Rheum., 2009;60(10):2892-901.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
Expand to show all 11 Citations
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Average Rating: 3 (Based on 2 reviews)

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 Human/Mouse/Rat Neuropilin‑2 Antibody AF2215-SP
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 Human/Mouse/Rat Neuropilin‑2 Antibody AF2215-SP
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedNeonatal dorsal root ganglia cells
SpeciesMouse

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