Human/Mouse/Rat Nrf2 Antibody

(6 citations)
(2 Reviews)
  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat Nrf2 in Western blots. In Western blots, no cross-reactivity with recombinant human Nrf1 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 383727
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Nrf2
    Met17-Asn605
    Accession # Q16236
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Nrf2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-), treated (+) or mock treated (m) with 10 µM MG132 (Catalog # 1748) for 4 hours and 10 pmol siRNA. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Nrf2 Monoclonal Antibody (Catalog # MAB3925) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Nrf2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nrf2

NF-E2 related factor 2 (Nrf2) is a member of the cap ‘n’ collar family of basic leucine zipper transcription factors. By binding antioxidant response elements (AREs), Nrf2 activates the transcription of genes encoding antioxidant and phase II drug-metabolizing enzymes needed to protect cells against oxidative damage. Transcriptional targets regulated by Nrf2 include genes encoding glutathione S-transferases, superoxide dismutase-1, and heme oxygenase-1.

  • Long Name:
    NF-E2-related Factor 2
  • Entrez Gene IDs:
    4780 (Human); 18024 (Mouse); 83619 (Rat)
  • Alternate Names:
    HEBP1; NFE2L2; NF-E2-related factor 2; NFE2-related factor 2; NRF2 erythroid derived 2, like 2; Nrf2; nuclear factor (erythroid-derived 2)-like 2; nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Cervical Cancer Cell Line Secretome Highlights the Roles of Transforming Growth Factor-Beta-Induced Protein ig-h3, Peroxiredoxin-2, and NRF2 on Cervical Carcinogenesis
    Authors: G Kontostath, J Zoidakis, M Makridakis, V Lygirou, G Mermelekas, T Papadopoul, K Vougas, A Vlamis-Gar, P Drakakis, D Loutradis, A Vlahou, NP Anagnou, KI Pappa
    Biomed Res Int, 2017;2017(0):4180703.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Paeoniflorin Attenuated Oxidative Stress in Rat COPD Model Induced by Cigarette Smoke
    Evid Based Complement Alternat Med, 2016;2016(0):1698379.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
  3. Sestrin 2 protein regulates platelet-derived growth factor receptor beta (Pdgfrbeta) expression by modulating proteasomal and Nrf2 transcription factor functions.
    Authors: Tomasovic A, Kurrle N, Surun D, Heidler J, Husnjak K, Poser I, Schnutgen F, Scheibe S, Seimetz M, Jaksch P, Hyman A, Weissmann N, von Melchner H
    J Biol Chem, 2015;290(15):9738-52.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. Vitiligo-inducing phenols activate the unfolded protein response in melanocytes resulting in upregulation of IL6 and IL8.
    J. Invest. Dermatol., 2012;132(11):2601-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Dimethyl fumarate, an immune modulator and inducer of the antioxidant response, suppresses HIV replication and macrophage-mediated neurotoxicity: a novel candidate for HIV neuroprotection.
    Authors: Cross SA, Cook DR, Chi AW, Vance PJ, Kolson LL, Wong BJ, Jordan-Sciutto KL, Kolson DL
    J. Immunol., 2011;187(10):5015-25.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. The drug monosodium luminol (GVT) preserves crypt-villus epithelial organization and allows survival of intestinal T cells in mice infected with the ts1 retrovirus.
    Authors: Scofield VL, Yan M, Kuang X, Kim SJ, Wong PK
    Immunol. Lett., 2009;122(2):150-8.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Mouse IgG2B Antibody

Flow MAB0043  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Average Rating: 3 (Based on 2 reviews)

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We have 1 review tested in 1 species: Mouse.
We have 2 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human/Mouse/Rat Nrf2 Antibody MAB3925
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 WB Mouse Anonymous 02/06/2017
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Western Blot Human/Mouse/Rat Nrf2 Antibody MAB3925
Western Blot: Human/Mouse/Rat Nrf2 Antibody [MAB3925]

Summary

ApplicationWestern Blot
Sample TestedFL5-12
SpeciesMouse
Western Blot Human/Mouse/Rat Nrf2 Antibody MAB3925
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 WB Anonymous 04/25/2016
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Western Blot Human/Mouse/Rat Nrf2 Antibody MAB3925
Western Blot: Human/Mouse/Rat Nrf2 Antibody [MAB3925]

Summary

ApplicationWestern Blot
Sample Testedmc-1 cell line

Other Experimental Details

Other Experimental DetailsRMC-1 cells (rat Muller cells) were cultured and treated, collect with NP-40 buffer, run WB. The problem is the molecular weight, we only see two specific bands around 75 KD marker, we didn't see any band around 120Kd as datasheet from R&D even longer exposure time. We would like to know why. I would like to provide more detailed information if you want to know. Thanks a lot. R&D Systems Technical Service is investigating.

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