Human/Mouse/Rat Nrf2 Antibody

  (8 citations)
(4 Reviews)
    
Datasheet / CoA / SDS
Product Details
Citations (8)
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat Nrf2 in Western blots. In Western blots, no cross-reactivity with recombinant human Nrf1 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 383727
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Nrf2
    Met17-Asn605
    Accession # Q16236
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Human Nrf2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-), treated (+) or mock treated (m) with 10 µM MG132 (Catalog # 1748) for 4 hours and 10 pmol siRNA. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Nrf2 Monoclonal Antibody (Catalog # MAB3925) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Nrf2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nrf2

NF-E2 related factor 2 (Nrf2) is a member of the cap ‘n’ collar family of basic leucine zipper transcription factors. By binding antioxidant response elements (AREs), Nrf2 activates the transcription of genes encoding antioxidant and phase II drug-metabolizing enzymes needed to protect cells against oxidative damage. Transcriptional targets regulated by Nrf2 include genes encoding glutathione S-transferases, superoxide dismutase-1, and heme oxygenase-1.

  • Long Name:
    NF-E2-related Factor 2
  • Entrez Gene IDs:
    4780 (Human); 18024 (Mouse); 83619 (Rat)
  • Alternate Names:
    HEBP1; NFE2L2; NF-E2-related factor 2; NFE2-related factor 2; NRF2 erythroid derived 2, like 2; Nrf2; nuclear factor (erythroid-derived 2)-like 2; nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Histone H2AX deficiency causes neurobehavioral deficits and impaired redox homeostasis
    Authors: U Weyemi, BD Paul, AM Snowman, P Jailwala, A Nussenzwei, WM Bonner, SH Snyder
    Nat Commun, 2018;9(1):1526.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. Hepatoprotective effect of calculus bovis sativus on nonalcoholic fatty liver disease in mice by inhibiting oxidative stress and apoptosis of hepatocytes
    Authors: W He, Y Xu, C Zhang, J Lu, J Li, D Xiang, J Yang, M Chang, D Liu
    Drug Des Devel Ther, 2017;11(0):3449-3460.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. Cervical Cancer Cell Line Secretome Highlights the Roles of Transforming Growth Factor-Beta-Induced Protein ig-h3, Peroxiredoxin-2, and NRF2 on Cervical Carcinogenesis
    Authors: G Kontostath, J Zoidakis, M Makridakis, V Lygirou, G Mermelekas, T Papadopoul, K Vougas, A Vlamis-Gar, P Drakakis, D Loutradis, A Vlahou, NP Anagnou, KI Pappa
    Biomed Res Int, 2017;2017(0):4180703.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Paeoniflorin Attenuated Oxidative Stress in Rat COPD Model Induced by Cigarette Smoke
    Evid Based Complement Alternat Med, 2016;2016(0):1698379.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
  5. Sestrin 2 protein regulates platelet-derived growth factor receptor beta (Pdgfrbeta) expression by modulating proteasomal and Nrf2 transcription factor functions.
    Authors: Tomasovic A, Kurrle N, Surun D, Heidler J, Husnjak K, Poser I, Schnutgen F, Scheibe S, Seimetz M, Jaksch P, Hyman A, Weissmann N, von Melchner H
    J Biol Chem, 2015;290(15):9738-52.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. Vitiligo-inducing phenols activate the unfolded protein response in melanocytes resulting in upregulation of IL6 and IL8.
    J. Invest. Dermatol., 2012;132(11):2601-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Dimethyl fumarate, an immune modulator and inducer of the antioxidant response, suppresses HIV replication and macrophage-mediated neurotoxicity: a novel candidate for HIV neuroprotection.
    Authors: Cross SA, Cook DR, Chi AW, Vance PJ, Kolson LL, Wong BJ, Jordan-Sciutto KL, Kolson DL
    J. Immunol., 2011;187(10):5015-25.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  8. The drug monosodium luminol (GVT) preserves crypt-villus epithelial organization and allows survival of intestinal T cells in mice infected with the ts1 retrovirus.
    Authors: Scofield VL, Yan M, Kuang X, Kim SJ, Wong PK
    Immunol. Lett., 2009;122(2):150-8.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 34  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 32  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 12  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow , IHC , ICC NL007 10
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 4  
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG Antibody

WB AF007 4
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Mouse IgG2B Antibody

Flow MAB0043  
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Average Rating: 3.3 (Based on 4 reviews)

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Read what people are saying who have used Human/Mouse/Rat Nrf2 Antibody.

We have 4 reviews tested in 4 species: Human, Mouse, Mouse,Rat, Rat.
We have 4 reviews tested in 2 applications: Western Blot, Immunocytochemistry/Immunofluorescence.

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(3)
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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human/Mouse/Rat Nrf2 Antibody MAB3925 Array MAB3925
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Very Good
 WB Mouse,Rat Anonymous 12/21/2017
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Image Details
Western Blot Human/Mouse/Rat Nrf2 Antibody MAB3925
Western Blot: Human/Mouse/Rat Nrf2 Antibody [MAB3925].

Summary

Application Western Blot
Sample Tested Primary hippocampal neurons
Species Mouse,Rat
Immunocytochemistry/Immunofluorescence Human/Mouse/Rat Nrf2 Antibody MAB3925 Array MAB3925
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Average
 ICC/IF Human Anonymous 12/10/2017
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Immunocytochemistry/Immunofluorescence Human/Mouse/Rat Nrf2 Antibody MAB3925
Immunocytochemistry/Immunofluorescence: Human/Mouse/Rat Nrf2 Antibody [MAB3925].

Summary

Application Immunocytochemistry/Immunofluorescence
Sample Tested Kidney cancer tissue
Species Human
Western Blot Human/Mouse/Rat Nrf2 Antibody MAB3925 Array MAB3925
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Average
 WB Mouse Anonymous 02/06/2017
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Image Details
Western Blot Human/Mouse/Rat Nrf2 Antibody MAB3925
Western Blot: Human/Mouse/Rat Nrf2 Antibody [MAB3925].

Summary

Application Western Blot
Sample Tested FL5-12
Species Mouse
Western Blot Human/Mouse/Rat Nrf2 Antibody MAB3925 Array MAB3925
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Average
 WB Rat Anonymous 04/25/2016
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Western Blot Human/Mouse/Rat Nrf2 Antibody MAB3925
Western Blot: Human/Mouse/Rat Nrf2 Antibody [MAB3925].

Summary

Application Western Blot
Sample Tested mc-1 cell line
Species Rat

Other Experimental Details

Other Experimental Details RMC-1 cells (rat Muller cells) were cultured and treated, collect with NP-40 buffer, run WB. The problem is the molecular weight, we only see two specific bands around 75 KD marker, we didn't see any band around 120Kd as datasheet from R&D even longer exposure time. We would like to know why. I would like to provide more detailed information if you want to know. Thanks a lot. R&D Systems Technical Service is investigating.

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