Human/Mouse/Rat p38 gamma Antibody

Name: Human/Mouse/Rat p38 gamma Antibody
Brand: R&D Systems
SKU: AF1347
Price: 159 USD
Availability: InStock

R&D Systems | Catalog # AF1347

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Citations (16)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all p38 gamma Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human p38 gamma
Accession # P53778

Specificity

Detects human, mouse, and rat p38 gamma in Western blots. The antibody is highly specific for p38 gamma and in Western blots, shows extremely weak cross-reactivity with recombinant p38 alpha, p38 beta, or p38 delta after prolonged exposure to film.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat p38 gamma Antibody

Detection of Mouse/Rat p38 gamma by Western Blot.

Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p38 gamma was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

p38 gamma in MCF‑7 Human Cell Line.

p38 gamma was detected in immersion fixed MCF-7 human breast cancer cell line using Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of human p38 gamma by Western Blot.

Western blot shows recombinant human p38 beta, p38 gamma, p38d and p38a (1 ng/lane). PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p38 gamma was detected at approx-imately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse and Rat p38 gamma by Simple Western^TM.

Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and PC‑12 rat adrenal pheochromocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for p38 gamma at approximately 48 kDa (as indicated) using 2 µg/mL of Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Porcine p38 gamma/SAPK3 by Western Blot

MAPK expression in normal and diabetic rat inferior vena cava. Immunoblot analyses indicating protein content of A) p44/42-MAPK, B) p38-MAPK, and C) JNK-MAPK in venae cavae from non-diabetic lean Zucker (LNZ) and diabetic obese syndrome X Zucker (OSXZ) rats. * Significantly different from the non-diabetic venae cavae, (P < 0.05). n = 6/group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/16961925), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Rat p38 gamma/SAPK3 by Western Blot

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Applications for Human/Mouse/Rat p38 gamma Antibody

Application

Recommended Usage

Immunocytochemistry

3-15 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast, human skin cancer tissue, and mouse skeletal muscle

Simple Western

2 µg/mL
Sample: C2C12 mouse myoblast cell line and PC‑12 rat adrenal pheochromocytoma cell line

Western Blot

0.2 µg/mL
Sample: PC-12 rat adrenal pheochromocytoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: p38 gamma

p38 mitogen-activated protein kinase (MAPK)gamma, also known as p38 gamma, MAPK12, SAPK3 and ERK6 is a serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays a role in myoblast differentiation and also in the
down-regulation of cyclin D1 in response to hypoxia in adrenal cells.