Human/Mouse/Rat Pannexin-1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human, Mouse, and Rat Pannexin‑1 by Western Blot.
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Product Details
Citations (4)
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Human/Mouse/Rat Pannexin-1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human Pannexin-1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Pannexin-2 or
-3 is observed.
Monoclonal Mouse IgG2B Clone # 720505
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Pannexin-1
Accession # Q96RD7
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Pannexin-1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Pannexin‑1 by Western Blot. Western blot shows lysates of human brain tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Pannexin-1 Monoclonal Antibody (Catalog # MAB7097) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Pannexin-1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pannexin-1

Pannexin-1 (PANX1) is an approximately 45 kDa member of the Pannexin family of four-transmembrane channel proteins with a conserved pattern of cysteines. It is expressed on epithelial cells, neuronal and glial cells, cardiac myocytes, T cells, and erythrocytes. Pannexin-1 forms large pore hexameric channels or heteromeric channels with Pannexin-2 and enables the efflux of ATP and UTP. Its conductance is increased in response to hypoxia, isotonic stress, NMDA R activation, and Caspase-3 and -7 activation during apopotosis. Extracellular release of ATP through Pannexin-1 activates P2X7 receptors which in turn induces the closure of Pannexin-1 channels. Pannexin-1 cooperates with P2X7 for inflammasome activation, Caspase-1 activation, and the release of mature IL-1 beta and also regulates T cell activation at the immunological synapse. Within aa 298-376 (in the C-terminal cytoplasmic domain), human Pannexin-1 shares 96% and 98% aa sequence identity with mouse and rat Pannexin-1, respectively. Alternate splicing generates an additional isoform that lacks the C-terminal four amino acids in the cytoplasmic tail.

Entrez Gene IDs
24145 (Human); 55991 (Mouse); 315435 (Rat)
Alternate Names
Innexin; MGC21309; MRS1; MRS1innexin; pannexin 1; Pannexin1; Pannexin-1; PANX1; PX1; UNQ2529

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Citations for Human/Mouse/Rat Pannexin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Acute Pannexin 1 Blockade Mitigates Early Synaptic Plasticity Defects in a Mouse Model of Alzheimer's Disease
    Authors: C Flores-Muñ, B Gómez, E Mery, P Mujica, I Gajardo, C Córdova, D Lopez-Espí, C Durán-Anio, C Hetz, P Muñoz, AM Gonzalez-J, ÁO Ardiles
    Front Cell Neurosci, 2020;14(0):46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. Adaptive thermogenesis in brown adipose tissue involves activation of pannexin-1 channels
    Authors: S Senthivina, V Serbulea, CM Upchurch, R Polanowska, SK Mendu, S Sahu, P Jayaguru, KW Aylor, MD Chordia, L Steinberg, N Oberholzer, S Uchiyama, N Inada, UM Lorenz, TE Harris, SR Keller, AK Meher, A Kadl, BN Desai, BK Kundu, N Leitinger
    Mol Metab, 2020;0(0):101130.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Novel evidence that extracellular nucleotides and purinergic signaling induce innate immunity-mediated mobilization of hematopoietic stem/progenitor cells
    Authors: M Adamiak, K Bujko, M Cymer, M Plonka, T Glaser, M Kucia, J Ratajczak, H Ulrich, A Abdel-Lati, MZ Ratajczak
    Leukemia, 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Human stem cells express pannexins
    Authors: N Hainz, A Beckmann, M Schubert, A Haase, U Martin, T Tschernig, C Meier
    BMC Res Notes, 2018;11(1):54.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot


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