Human/Mouse/Rat Phospho-p70 S6 Kinase (T421/S424) Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8965
MAB8965-SP
Detection of Human Phospho-p70 S6 Kinase by Western Blot.
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Human/Mouse/Rat Phospho-p70 S6 Kinase (T421/S424) Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human p70 S6 Kinase when phosphorylated at T421/S424.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1143C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Phosphopeptide containing the human p70 S6 Kinase T421/S424 site
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Phospho-p70 S6 Kinase by Western Blot. View Larger

Detection of Human Phospho-p70 S6 Kinase by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 1 hour. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human/Mouse/Rat Phospho-p70 S6 Kinase (T421/S424) Monoclonal Antibody (Catalog # MAB8965) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phopsho-p70 S6 Kinase (T421/S424) at approximately 70 kDa and Phospho-p85 S6 Kinase (T444/S447) at 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
  • 12 months from date of receipt, -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 °C under sterile conditions after opening.

Background: p70 S6 Kinase

p70 S6 Kinase (p70S6K) is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues (1). p70S6K is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6K activity undergoes changes in the cell cycle and increases 20-fold in G1 cells released from G0 (2). p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well as T389, a site phosphorylated by Phosphoinositide-Dependent Kinase 1 (PDK1).

References
  1. Ferrari, S. et al. (1994) Crit. Rev. Biochem. Mol. Biol. 29:385.
  2. Edelmann, H.M. et al. (1996) J. Biol. Chem. 271:963.
Entrez Gene IDs
6198 (Human); 72508 (Mouse); 83840 (Rat)
Alternate Names
EC 2.7.11; EC 2.7.11.1; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6 Kinase; p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1; p70 S6 kinase, alpha 2; p70 S6KA; p70 S6K-alpha; p70(S6K)-alpha; p70-alpha; p70-S6K; P70S6K1; PS6K; ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kD, polypeptide 1; ribosomal protein S6 kinase, 70kDa, polypeptide 1; RPS6KB1; S6K; S6K1; S6K1p70-S6K 1; S6K-beta-1; serine/threonine kinase 14 alpha; Serine/threonine-protein kinase 14A; STK14A

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