Human/Mouse/Rat PI 3‑Kinase p85 alpha Antibody

Name: Human/Mouse/Rat PI 3‑Kinase p85 alpha Antibody
Brand: R&D Systems
SKU: AF2998
Price: 159 USD
Availability: InStock

R&D Systems | Catalog # AF2998

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all PI 3-Kinase p85 alpha Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PI 3-Kinase p85 alpha
Leu328-Tyr431
Accession # P27986

Specificity

Detects endogenous human, mouse and rat PI 3-Kinase p85 alpha in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat PI 3‑Kinase p85 alpha Antibody

Detection of Human/Mouse/Rat PI 3-Kinase p85 alpha by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PI 3‑Kinase p85 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2998) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PI 3‑Kinase p85 alpha at approximately 85 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat PI 3‑Kinase p85 alpha Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, mouse brain tissue, and rat brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PI 3-Kinase p85 alpha

Class I phosphoinositide 3-kinases (PI 3-Kinases) are heterodimeric proteins that consist of a catalytic subunit of 110‑120 kDa and an associated regulatory subunit. p85 alpha is one of the regulatory subunits that associate with the class IA PI 3-Kinases. Composed of one SH3 and two SH2 domains, p85 alpha functions as an adapter, coupling catalytic p110 to activated receptor tyrosine kinases.