Detection of Human/Mouse/Rat PI 3-Kinase p85 alpha by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PI 3‑Kinase p85 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2998) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PI 3‑Kinase p85 alpha at approximately 85 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PI 3-Kinase p85 alpha
Class I phosphoinositide 3-kinases (PI 3-Kinases) are heterodimeric proteins that consist of a catalytic subunit of 110‑120 kDa and an associated regulatory subunit. p85 alpha is one of the regulatory subunits that associate with the class IA PI 3-Kinases. Composed of one SH3 and two SH2 domains, p85 alpha functions as an adapter, coupling catalytic p110 to activated receptor tyrosine kinases.
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