Human/Mouse/Rat PTEN Antibody

Catalog # Availability Size / Price Qty
AF847
AF847-SP
Detection of Human/Mouse/Rat PTEN by Western Blot.
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Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse/Rat PTEN Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat PTEN in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Human PTEN synthetic peptide
Ser385-Val403
Accession # P60484
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
See below
Immunohistochemistry
3-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below
Knockout Validated
PTEN is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in PTEN knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human/Mouse/Rat PTEN antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat PTEN by Western Blot. Western blot shows lysates of mouse and rat brain tissue, A431 human epithelial carcinoma cell line, and MRC-5 human embryonic lung fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL Rabbit Anti-Human/Mouse/Rat PTEN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF847) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, recombinant human PTEN (5 ng) was included. A specific band for PTEN was detected at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Immunohistochemistry View Larger

PTEN in Human Pancreas. PTEN was detected in immersion fixed paraffin-embedded sections of human pancreas using Rabbit Anti-Human/Mouse/Rat PTEN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF847) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in islet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry View Larger

PTEN in Mouse Pancreas. PTEN was detected in immersion fixed paraffin-embedded sections of mouse pancreas using Rabbit Anti-Human/Mouse/Rat PTEN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF847) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in islet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Intracellular Staining by Flow Cytometry Detection of PTEN antibody in Human PBMC lymphocytes antibody by Flow Cytometry. View Larger

Detection of PTEN in Human PBMC lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Rabbit Anti-Human/Mouse/Rat PTEN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF847, filled histogram) or control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Simple Western Detection of Human PTEN antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human PTEN by Simple WesternTM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTEN at approximately 60 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human/Mouse/Rat PTEN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF847). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated Western Blot Shows Human PTEN Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human PTEN Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and PTEN knockout HeLa cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat PTEN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF847) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PTEN at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTEN

The tumor suppressor gene PTEN (phosphatase and tensin homolog deleted on chromosome 10), also known as MMAC1 (mutated in multiple advanced cancers 1), encodes a phosphatase that contains the catalytic signature motif (HCXXGXXRS/T) found in all members of the protein tyrosine phosphatase family. In vitro, the recombinant PTEN has both lipid phosphatase and protein phosphatase activities (1, 2). Interestingly, accumulating evidence has shown that the tumor suppressor activity of PTEN relies on its ability to dephosphorylate phosphatidylinositol (3, 4, 5)-triphosphate specifically at position 3 of the inositol ring (3). This activity reduces the levels of phosphatidylinositol (3, 4, 5)-triphosphate which is specifically produced from phosphatidylinositol (4, 5)-diphosphate by PI 3-kinase upon activation by a variety of stimuli. Therefore, PTEN antagonizes PI 3-kinase-induced downstream signaling events and cellular processes including cell growth, apoptosis and cell motility. In vivo, the importance of PTEN catalytic activity in its tumor suppressor functions is underscored by the fact that the majority of PTEN missense mutations detected in tumor specimens target the phosphatase domain and cause a loss in PTEN phosphatase activity (4).

References
  1. Maehama, T. and J. Dixon (1998) J. Biol. Chem. 273:13375.
  2. Das, S. et al. (2003) Proc. Natl. Acad. Sci. USA 100:7491.
  3. Myers, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13513.
  4. Waite, K. and C. Eng (2002) Am. J. Hum. Genet. 70:829.
Long Name
Phosphatase and Tensin Homolog Deleted on Chromosome 10
Entrez Gene IDs
5728 (Human); 19211 (Mouse); 50557 (Rat)
Alternate Names
BZS; EC 3.1.3.16,10q23del; EC 3.1.3.48; EC 3.1.3.67; GLM2; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; MMAC1; MMAC1MGC11227; Mutated in multiple advanced cancers 1; phosphatase and tensin homologDEC; phosphatidylinositol-34,5-trisphosphate 3-phosphatase and dual-specificityprotein phosphatase PTEN; PTEN; PTEN1; TEP1MHAM

Product Datasheets

Product Specific Notices

This product is covered by the following U.S. patent: USSN # 10/299,003.

Citations for Human/Mouse/Rat PTEN Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Nickel's Role in Pancreatic Ductal Adenocarcinoma: Potential Involvement of microRNAs
    Authors: M Mortoglou, L Mani?, A Buha Djord, Z Bulat, V ?or?evi?, K Manis, E Valle, L York, D Wallace, P Uysal-Onga
    Toxics, 2022;10(3):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ELISA Development
  2. A scalable Drosophila assay for clinical interpretation of human PTEN variants in suppression of PI3K/AKT induced cellular proliferation
    Authors: P Ganguly, L Madonsela, JT Chao, CJR Loewen, TP O'Connor, EM Verheyen, DW Allan
    PloS Genetics, 2021;17(9):e1009774.
    Species: Drosophila
    Sample Types: Whole Tissue
    Applications: IHC
  3. TGF-? induces phosphorylation of phosphatase and tensin homolog: implications for fibrosis of the trabecular meshwork tissue in glaucoma
    Authors: N Tellios, JC Belrose, AC Tokarewicz, C Hutnik, H Liu, A Leask, M Motolko, M Iijima, SK Parapuram
    Sci Rep, 2017;7(1):812.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Biomarker analysis of the NeoSphere study: pertuzumab, trastuzumab, and docetaxel versus trastuzumab plus docetaxel, pertuzumab plus trastuzumab, or pertuzumab plus docetaxel for the neoadjuvant treatment of HER2-positive breast cancer
    Authors: G Bianchini, A Kiermaier, GV Bianchi, YH Im, T Pienkowski, MC Liu, LM Tseng, M Dowsett, L Zabaglo, S Kirk, T Szado, J Eng-Wong, LC Amler, P Valagussa, L Gianni
    Breast Cancer Res, 2017;19(1):16.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. A novel role for GSK3? as a modulator of Drosha microprocessor activity and MicroRNA biogenesis
    Nucleic Acids Res, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Tumor suppressor PTEN in breast cancer: heterozygosity, mutations and protein expression.
    Authors: Kechagioglou, Petros, Papi, Rigini M, Provatopoulou, Xeni, Kalogera, Eleni, Papadimitriou, Elli, Grigoropoulos, Petros, Nonni, Aphrodit, Zografos, George, Kyriakidis, Dimitrio, Gounaris, Antonia
    Anticancer Res, 2014;34(3):1387-400.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Basal activation of p70S6K results in adipose-specific insulin resistance in protein-tyrosine phosphatase 1B -/- mice.
    Authors: Ruffolo SC, Forsell PK, Yuan X, Desmarais S, Himms-Hagen J, Cromlish W, Wong KK, Kennedy BP
    J. Biol. Chem., 2007;282(42):30423-33.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human/Mouse/Rat PTEN Antibody
By Punitha Senthil on 01/12/2018
Application: Western Blot Sample Tested: PANC-1 human pancreatic carcinoma cell line Species: Human