Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, and CHP‑100 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat PTPμ/PTPRM Monoclonal Antibody (Catalog # MAB4446) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTPμ/PTPRM at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTP mu/PTPRM
Protein Tyrosine Phosphatase, receptor type M (PTPRM), also called PTPμ, PTPRμ, and RPTPμ, is expressed at highest levels in pulmonary vascular epithelia, where interactions with cadhedrins are believed to be important in regulating barrier permeability. The MAM and Fibronectin III (FNIII) domains on the extracellular side of PTPRM bind between cells, affecting adhesion and contact inhibition. Culturing cells to high density concentrates PTPRM at sites of tight contact and induces proteolytic cleavage of the 100 kDa extracellular domain.
Protein Tyrosine Phosphatase Receptor-type M
Entrez Gene IDs:
5797 (Human); 19274 (Mouse); 29616 (Rat)
EC 126.96.36.199; hR-PTPu; MGC166994; protein tyrosine phosphatase mu; protein tyrosine phosphatase, receptor type, M; protein tyrosine phosphatase, receptor type, mu polypeptide; Protein-tyrosine phosphatase mu; PTPRL1; PTPRL1R-PTP-MU; PTPRM; receptor-type tyrosine-protein phosphatase mu; RPTPM; R-PTP-mu; RPTPU
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