PTP mu/PTPRM Antibody (436502)

Detection of Human PTPμ/PTPRM by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, and CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat PTPµ/PTPRM Monoclonal Antibody (Catalog # MAB4446) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTPµ/PTPRM at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of PTP mu/PTPRM by Western Blot

DDIAS inhibits the interaction between STAT3 and PTPRM.a mRNA levels of PTPRM, PTPRT, SHP1, and TC-PTP in DDIAS-knockdown cells. RT-qPCR was performed 72 h after siDDIAS transfection. All experiments were independently performed three times, and each experiment was performed in triplicate. b Western blot analysis of PTPRM, SHP1, and TC-PTP in DDIAS-knockdown cells. c Activity of total PTP in DDIAS-knockdown cells. d Interaction between PTPRM and STAT3 in the presence of IL-6 in DDIAS-knockdown cells. Immunoprecipitation was performed using anti-STAT3 antibody. e Interaction between Myc-PTPRM and Flag-DDIAS. Immunoprecipitation was performed using anti-Myc antibody. f PTPRM expression in different NSCLC cell lines. g DDIAS, PTPRM, and pSTAT3 (Y705) expression in a human lung cancer tissue array. Representative images of LC tissue stained with an anti-DDIAS (1:100), anti-PTPRM (1:200), and anti-pSTAT3 antibodies (1:100). Scale bar, 500 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31900385), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of PTP mu/PTPRM by Western Blot

DDIAS inhibits the interaction between STAT3 and PTPRM.a mRNA levels of PTPRM, PTPRT, SHP1, and TC-PTP in DDIAS-knockdown cells. RT-qPCR was performed 72 h after siDDIAS transfection. All experiments were independently performed three times, and each experiment was performed in triplicate. b Western blot analysis of PTPRM, SHP1, and TC-PTP in DDIAS-knockdown cells. c Activity of total PTP in DDIAS-knockdown cells. d Interaction between PTPRM and STAT3 in the presence of IL-6 in DDIAS-knockdown cells. Immunoprecipitation was performed using anti-STAT3 antibody. e Interaction between Myc-PTPRM and Flag-DDIAS. Immunoprecipitation was performed using anti-Myc antibody. f PTPRM expression in different NSCLC cell lines. g DDIAS, PTPRM, and pSTAT3 (Y705) expression in a human lung cancer tissue array. Representative images of LC tissue stained with an anti-DDIAS (1:100), anti-PTPRM (1:200), and anti-pSTAT3 antibodies (1:100). Scale bar, 500 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31900385), licensed under a CC-BY license. Not internally tested by R&D Systems.