Human/Mouse/Rat RIPK1/RIP1 Antibody

  (5 citations)     
Datasheet / CoA / SDS
Product Details
Citations (5)
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  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse and rat RIPK1/RIP1 in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 334640
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human RIPK1/RIP1
    Met1-Asn671
    Accession # Q13546
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse/Rat RIPK1/RIP1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, DA3 mouse myeloma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat RIPK1/RIP1 Monoclonal Antibody (Catalog # MAB3585) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RIPK1/RIP1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Immunocytochemistry
RIPK1/RIP1 in MCF‑7 Human Cell Line. RIPK1/RIP1 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat RIPK1/RIP1 Monoclonal Antibody (Catalog # MAB3585) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human RIPK1/RIP1 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for RIPK1/RIP1 at approximately 78 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat RIPK1/RIP1 Monoclonal Antibody (Catalog # MAB3585). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RIPK1/RIP1

Receptor-Interacting Protein 1 (RIP1, also known as RIPK1) is a 671 amino acid (aa) 75 kDa protein that contains an N-terminal protein kinase domain, a C-terminal death domain, and a unique internal region called the intermediate domain. RIP1 is a serine/threonine protein kinase and is constitutively expressed in many tissues. RIP1 interacts with the cytoplasmic death domain of FAS and TNF receptors and is an important element in the signal transduction machinery that mediates apoptosis. RIP1 has been shown to interact with a number of proteins including TRADD, TRAF1, TRAF2, and TRAF3, to form larger signaling complexes. These complexes, in turn, activate specific signaling cascades, such as NF kappa B. RIP1 also interacts through the C-terminal RIP homotypic interaction motif (RHIM) of TRIF in TLR3 dependent activation of NF kappa B.

  • Long Name:
    Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 1
  • Entrez Gene IDs:
    8737 (Human); 19766 (Mouse)
  • Alternate Names:
    Cell death protein RIP; EC 2.7.11.1; receptor (TNFRSF)-interacting serine-threonine kinase 1; receptor interacting protein; Receptor-interacting protein 1; receptor-interacting serine/threonine-protein kinase 1; RIP; Rip1 kinase; RIP1; RIP-1; RIPFLJ39204; RIPK1; Serine/threonine-protein kinase RIP
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. MPP+ induces necrostatin-1- and ferrostatin-1-sensitive necrotic death of neuronal SH-SY5Y cells
    Authors: K Ito, Y Eguchi, Y Imagawa, S Akai, H Mochizuki, Y Tsujimoto
    Cell Death Discov, 2017;3(0):17013.
    Species: Human
    Sample Type: Cell Lystaes
    Application: WB
  2. Hypoxia potentiates the cytotoxic effect of piperlongumine in pheochromocytoma models
    Oncotarget, 2016;7(26):40531-40545.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. ESCRT-0 dysfunction compromises autophagic degradation of protein aggregates and facilitates ER stress-mediated neurodegeneration via apoptotic and necroptotic pathways
    Authors: R Oshima, T Hasegawa, K Tamai, N Sugeno, S Yoshida, J Kobayashi, A Kikuchi, T Baba, A Futatsugi, I Sato, K Satoh, A Takeda, M Aoki, N Tanaka
    Sci Rep, 2016;6(0):24997.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  4. Open reading frame 3 of genotype 1 hepatitis E virus inhibits nuclear factor-kappaappa B signaling induced by tumor necrosis factor-alpha in human A549 lung epithelial cells.
    Authors: Xu J, Wu F, Tian D, Wang J, Zheng Z, Xia N
    PLoS ONE, 2014;9(6):e100787.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. TWEAK Induces Apoptosis through a Death-signaling Complex Comprising Receptor-interacting Protein 1 (RIP1), Fas-associated Death Domain (FADD), and Caspase-8.
    Authors: Ikner A, Ashkenazi A
    J. Biol. Chem., 2011;286(24):21546-54.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

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