Detection of Human and Rat Src by Western Blot.
Western blot shows lysates of MCF‑7 human breast cancer cell line, Y3‑Ag rat myeloid cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Src Monoclonal Antibody (Catalog # MAB3389) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Src at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
The Src family of proteins are intracellular tyrosine kinases involved in cell proliferation, differentiation, motility, and survival. Src family activity is regulated by tyrosine phosphorylation at two sites with opposing effects. Autophosphorylation in the activation loop of the kinase domain (Y419 of human Src) upregulates the enzyme, while phosphorylation in the C-terminal tail (Y530 of human Src) downregulates activity.
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