Human/Mouse/Rat VAMP-2 Antibody

Catalog # Availability Size / Price Qty
Detection of Human, Mouse, and Rat VAMP‑2 by Western Blot.
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Product Details
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Human/Mouse/Rat VAMP-2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse, and rat VAMP-2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) VAMP-1 is observed and less than 2% cross-reactivity with rhVAMP-3 and rhVAMP-7 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human VAMP-2
Accession # P63027
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat VAMP-2 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat VAMP‑2 by Western Blot. Western blot shows lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line, SH-SY5Y human neuroblastoma cell line, mouse brain tissue, and rat testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat VAMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5136) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VAMP-2 at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry VAMP-2 antibody in Rat Spinal Cord by Immunohistochemistry (IHC-Fr). View Larger

VAMP-2 in Rat Spinal Cord. Vesicle-Associate Membrane Protein 2 (VAMP-2) was detected in perfusion-fixed frozen sections of rat spinal cord using a Goat Anti-Human/Mouse/Rat VAMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5136). The cells were stained with the NorthernLights 557-conjugated Donkey Anti-Goat IgG Affinity-purified Secondary Antibody (red; Catalog # NL001). The cell nuclei were counterstained with DAPI (blue). VAMP-2 immunoreactivity was localized to synaptic vesicles. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VAMP-2

VAMP-2 (vesicle associated membrane protein 2; also synaptobrevin-2) is a 13 kDa member of the synaptobrevin family of proteins. It is a type IV transmembrane (TM) protein (i.e.- a type II TM protein whose C-terminus is almost completely transmembrane) that is found in the presynaptic terminals of neurons. VAMP-2 is targeted to presynaptic vesicles following binding to synaptophysin I. Dissociation allows for synaptic vesicle fusion at the synaptic cleft with subsequent granule release. Human VAMP-2 is 116 amino acids (aa) in length. It contains one acetylation site at Ser2, a vSNARE coiled-coil homology region (aa 31‑91), and a membrane-anchor domain (aa 95‑114). Over aa 1‑94, human VAMP-2 shares 100% and 99% aa identity with canine and mouse VAMP-2, respectively.

Long Name
Vesicle-Associated Membrane Protein 2
Entrez Gene IDs
6844 (Human); 22318 (Mouse); 24803 (Rat)
Alternate Names
FLJ11460; SYB2; SYB2vesicle-associated membrane protein 2; synaptobrevin 2; synaptobrevin-2; VAMP2; VAMP-2; VAMP-2vesicle associated membrane protein 2; vesicle-associated membrane protein 2 (synaptobrevin 2)

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