Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all VAMP-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human VAMP-2
Met1-Lys94
Accession # P63027

Specificity

Detects human, mouse, and rat VAMP-2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) VAMP-1 is observed and less than 2% cross-reactivity with rhVAMP-3 and rhVAMP-7 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat VAMP‑2 Antibody

Detection of Human, Mouse, and Rat VAMP-2 antibody by Western Blot.

Detection of Human, Mouse, and Rat VAMP‑2 by Western Blot.

Western blot shows lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line, SH-SY5Y human neuroblastoma cell line, mouse brain tissue, and rat testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat VAMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5136) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VAMP-2 at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

VAMP-2 antibody in Rat Spinal Cord by Immunohistochemistry (IHC-Fr).

VAMP-2 in Rat Spinal Cord.

Vesicle-Associate Membrane Protein 2 (VAMP-2) was detected in perfusion-fixed frozen sections of rat spinal cord using a Goat Anti-Human/Mouse/Rat VAMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5136). The cells were stained with the NorthernLights 557-conjugated Donkey Anti-Goat IgG Affinity-purified Secondary Antibody (red; Catalog # NL001). The cell nuclei were counterstained with DAPI (blue). VAMP-2 immunoreactivity was localized to synaptic vesicles. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Human/Mouse/Rat VAMP‑2 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat spinal cord

Western Blot

1 µg/mL
Sample: Nalm‑6 human Pre-B acute lymphocytic leukemia cell line, SH‑SY5Y human neuroblastoma cell line, mouse brain tissue, and rat testis tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VAMP-2

VAMP-2 (vesicle associated membrane protein 2; also synaptobrevin-2) is a 13 kDa member of the synaptobrevin family of proteins. It is a type IV transmembrane (TM) protein (i.e.- a type II TM protein whose C-terminus is almost completely transmembrane) that is found in the presynaptic terminals of neurons. VAMP-2 is targeted to presynaptic vesicles following binding to synaptophysin I. Dissociation allows for synaptic vesicle fusion at the synaptic cleft with subsequent granule release. Human VAMP-2 is 116 amino acids (aa) in length. It contains one acetylation site at Ser2, a vSNARE coiled-coil homology region (aa 31‑91), and a membrane-anchor domain (aa 95‑114). Over aa 1‑94, human VAMP-2 shares 100% and 99% aa identity with canine and mouse VAMP-2, respectively.

Long Name

Vesicle-Associated Membrane Protein 2

Alternate Names

SYB2, VAMP2

Entrez Gene IDs

6844 (Human); 22318 (Mouse); 24803 (Rat)

Gene Symbol

VAMP2

UniProt

P63027

Additional VAMP-2 Products

Product Documents for Human/Mouse/Rat VAMP‑2 Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat VAMP‑2 Antibody

For research use only

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Protocols

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