Human/Mouse/Rat YAP1 Antibody

MAB8094 2 Citations Datasheet / COA / SDS

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MAB8094
MAB8094-SP

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Human/Mouse/Rat YAP1 Antibody in Western Blot

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat YAP1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human YAP1 in ELISA. Detects human, mouse and rat YAP1 in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 867711

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human YAP1
Ala347-Leu504
Accession # P46937

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Simple Western

20 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

CyTOF-reported

Brodie, T.M. et al. (2018) Cytometry PartA. 93: 406. Ready to be labeled using establishedconjugation methods. No BSA or other carrier proteins that could interfere withconjugation.

Knockout Validated

YAP1 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in YAP1 knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot

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Detection of Human, Mouse, and Rat YAP1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human YAP1 Monoclonal Antibody (Catalog # MAB8094) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for YAP1 at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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YAP1 in Human Prostate. YAP1 was detected in formalin fixed paraffin-embedded sections of human prostate using Mouse Anti-Human YAP1 Monoclonal Antibody (Catalog # MAB8094) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the nuclei of smooth muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western

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Detection of Human YAP1 by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for YAP1 at approximately 79 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat YAP1 Monoclonal Antibody (Catalog # MAB8094). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated

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Western Blot Shows Human YAP1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat YAP1 Monoclonal Antibody (Catalog # MAB8094) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for YAP1 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: YAP1

YAP1 (Yes-associated protein 1), also called YAP65 or Yorkie Homolog, is a widely expressed 65 kDa cytoplasmic and nuclear transcriptional regulator protein in the Hippo pathway that regulates organ size. The 504 amino acid (aa) human YAP1 contains two WW (tryptophan-containing protein binding) domains (aa 171-263), a transactivation domain (aa 219-504) and 19 sites that may be phosphorylated by kinases such as LATS1 and 2, MAPK8 and 9, CK1, or ABL1. Isoforms of 488, 454 and 326 aa lack aa 328-343, 230-267 (plus a 3 aa substitution for aa 329-343), or 1-178, respectively. YAP1 binds the Yes tyrosine kinase SH3 domain and many other proteins, notably ErbB4. YAP1 expression is increased in some liver and prostate cancers. Within aa 347-504, human YAP1 shares 94% and 93% aa sequence identity with mouse and rat YAP1, respectively.

Long Name

Yes-associated Protein 1

Entrez Gene IDs

10413 (Human); 22601 (Mouse)

Alternate Names

65 kDa Yes-associated protein; COB1; Protein Yorkie Homolog; Transcriptional Coactivator YAP1; YAP; YAP1; YAP1-2gamma; YAP2; YAP2L; YAP65; YAP65yes-associated protein 2; Yes Associated Protein; Yes-associated protein 1; Yes-associated protein 1, 65kDa; yes-associated protein beta; yes-associated protein delta; Yes-Associated Protein YAP65 Homolog ; Yes-Associated Protein; YKI; Yorkie Homolog

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat YAP1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Antibody-drug conjugate T-DM1 treatment for HER2+ breast cancer induces ROR1 and confers resistance through activation of Hippo transcriptional coactivator YAP1
Authors: SS Islam, M Uddin, ASM Noman, H Akter, NJ Dity, M Basiruzzma, F Uddin, J Ahsan, S Annoor, AA Alaiya, M Al-Alwan, H Yeger, WA Farhat
EBioMedicine, 2019;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
A connexin43/YAP axis regulates astroglial-mesenchymal transition in hemoglobin induced astrocyte activation
Authors: Y Yang, J Ren, Y Sun, Y Xue, Z Zhang, A Gong, B Wang, Z Zhong, Z Cui, Z Xi, GY Yang, Q Sun, L Bian
Cell Death Differ., 2018;0(0):.
Species: Rat
Sample Types: Protein
Applications: Immunoprecipitation