Human/Mouse/Rat Yes Antibody Summary
Accession # P07947.3
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Rat Yes by Western Blot. Western blot shows lysates of Rat-2 rat embryonic fibroblast cell line and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Yes Monoclonal Antibody (Catalog # MAB32051) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Yes at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Yes in Human Breast Cancer Tissue. Yes was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Human/Mouse/Rat Yes Monoclonal Antibody (Catalog # MAB32051) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Yes is the cellular homolog of the oncogenic protein encoded by the Yamaguchi 73 and Esh avian sarcoma viruses. After Src, Yes is the most widely expressed member of the Src family of nonreceptor tyrosine kinases. Src family kinases regulate an array of cellular processes, including growth factor signaling, cytoskeleton dynamics, and cell proliferation.
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