Receptor-interacting serine-threonine kinase 4 (RIPK4/PKK) is a serine/threonine protein kinase which interacts with protein kinase C-delta, but also activates NFkappaB, and interacts with the Wnt3a coreceptor LRP6 after Wnt3a stimulation. RIPK4 is required for keratinocyte differentiation and has broad expression in epithelial tissues. Down regulation of RIPK4 has been observed in squamous cell carcinomas, hepatocellular carcinoma, urothelial carcinoma and cervical carcinoma. Mutations in in the RIPK4 gene cause Popliteal pterygium syndrome, lethal type (PPS-L), CHAND syndrome and Bartsocas-Papas syndrome.
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human/mouse RIPK4/RIP4
Thr218-Thr450
Accession # P57078
Thr218-Thr450
Accession # P57078
Specificity
Detects human and mouse RIPK4/RIP4 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse RIPK4/RIP4 Antibody
Detection of Human RIPK4/RIP4 by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line, PANC-1 human pancreatic carcinoma cell line, and SK-OV-3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse RIPK4/RIP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10002) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for RIPK4/RIP4 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
RIPK4/RIP4 in Human Kidney.
RIPK4/RIP4 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human/Mouse RIPK4/RIP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10002) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cells in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
RIPK4/RIP4 in Mouse Kidney.
RIPK4/RIP4 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Human/Mouse RIPK4/RIP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10002) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cells in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Applications for Human/Mouse RIPK4/RIP4 Antibody
Application
Recommended Usage
Immunohistochemistry
0.3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney, and perfusion fixed frozen sections of mouse kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney, and perfusion fixed frozen sections of mouse kidney
Western Blot
2 µg/mL
Sample: A549 human lung carcinoma cell line, PANC‑1 human pancreatic carcinoma cell line, and SK‑OV‑3 human ovarian adenocarcinoma cell line
Sample: A549 human lung carcinoma cell line, PANC‑1 human pancreatic carcinoma cell line, and SK‑OV‑3 human ovarian adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: RIPK4/RIP4
Long Name
Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 4
Alternate Names
ANKRD3, RIP4
Gene Symbol
RIPK4
UniProt
Additional RIPK4/RIP4 Products
Product Documents for Human/Mouse RIPK4/RIP4 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse RIPK4/RIP4 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars