Human/Mouse SOCS-4 Antibody Summary
Accession # Q8WXH5
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human/Mouse SOCS‑4 by Western Blot. Western blot shows lysates of NC-37 human Burkitt's lymphoma B lymphoblast cell line, HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse SOCS-4 Monoclonal Antibody (Catalog # MAB5628) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOCS-4 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Suppressor of cytokine signaling 4 (SOCS-4) is a 51 kDa (predicted) member of the SOCS family of proteins, which are Src homology-2 (SH2)-containing proteins originally identified as negative regulators of cytokine signaling. Human SOCS-4 is 440 amino acids (aa) in length. It contains an SH2 domain (aa 289‑381) and a SOCS box domain (aa 376‑425). Human SOCS-4 shares 93% and 86% aa sequence identity with bovine and mouse SOCS-4, respectively. The biological function of SOCS-4 remains poorly understood, but it has been found to be elevated in cells following treatment with EGF, acting perhaps in a feedback loop inhibiting STAT3.
Citation for Human/Mouse SOCS-4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
In vitro significance of SOCS-3 and SOCS-4 and potential mechanistic links to wound healing
Authors: Y Feng, AJ Sanders, LD Morgan, S Owen, F Ruge, KG Harding, WG Jiang
Sci Rep, 2017;7(1):6715.
Sample Types: Cell Lysates
Applications: Western Blot
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