Detection of Human/Mouse SOCS‑4 by Western Blot. Western blot shows lysates of NC‑37 human Burkitt's lymphoma B lymphoblast cell line, HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse SOCS‑4 Monoclonal Antibody (Catalog # MAB5628) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOCS‑4 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Suppressor of cytokine signaling 4 (SOCS-4) is a 51 kDa (predicted) member of the SOCS family of proteins, which are Src homology-2 (SH2)-containing proteins originally identified as negative regulators of cytokine signaling. Human SOCS-4 is 440 amino acids (aa) in length. It contains an SH2 domain (aa 289‑381) and a SOCS box domain (aa 376‑425). Human SOCS-4 shares 93% and 86% aa sequence identity with bovine and mouse SOCS-4, respectively. The biological function of SOCS-4 remains poorly understood, but it has been found to be elevated in cells following treatment with EGF, acting perhaps in a feedback loop inhibiting STAT3.
Suppressor Of Cytokine Signaling 4
Entrez Gene IDs:
122809 (Human); 67296 (Mouse); 305828 (Rat)
CIS4; DKFZp686J1568; SOCS4; SOCS-4; SOCS7; SOCS-7; SSI4; STAT4; suppressor of cytokine signaling 4; Suppressor of cytokine signaling 7SH2 domain containing SOCS box protein
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