Human/Mouse SOCS-4 Antibody

Catalog # Availability Size / Price Qty
MAB5628
MAB5628-SP
Human/Mouse SOCS-4 Antibody in Western Blot
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Product Details
Citations (1)
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Human/Mouse SOCS-4 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects endogenous human and mouse SOCS-4 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 517331
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human SOCS-4
Met1-Cys440
Accession # Q8WXH5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human/Mouse SOCS‑4 by Western Blot. View Larger

Detection of Human/Mouse SOCS‑4 by Western Blot. Western blot shows lysates of NC-37 human Burkitt's lymphoma B lymphoblast cell line, HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse SOCS-4 Monoclonal Antibody (Catalog # MAB5628) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOCS-4 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOCS-4

Suppressor of cytokine signaling 4 (SOCS-4) is a 51 kDa (predicted) member of the SOCS family of proteins, which are Src homology-2 (SH2)-containing proteins originally identified as negative regulators of cytokine signaling. Human SOCS-4 is 440 amino acids (aa) in length. It contains an SH2 domain (aa 289‑381) and a SOCS box domain (aa 376‑425). Human SOCS-4 shares 93% and 86% aa sequence identity with bovine and mouse SOCS-4, respectively. The biological function of SOCS-4 remains poorly understood, but it has been found to be elevated in cells following treatment with EGF, acting perhaps in a feedback loop inhibiting STAT3.

Long Name
Suppressor Of Cytokine Signaling 4
Entrez Gene IDs
122809 (Human); 67296 (Mouse); 305828 (Rat)
Alternate Names
CIS4; DKFZp686J1568; SOCS4; SOCS-4; SOCS7; SOCS-7; SSI4; STAT4; suppressor of cytokine signaling 4; Suppressor of cytokine signaling 7SH2 domain containing SOCS box protein

Product Datasheets

Citation for Human/Mouse SOCS-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. In vitro significance of SOCS-3 and SOCS-4 and potential mechanistic links to wound healing
    Authors: Y Feng, AJ Sanders, LD Morgan, S Owen, F Ruge, KG Harding, WG Jiang
    Sci Rep, 2017;7(1):6715.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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