Detection of Human Stathmin‑2/STMN2 by Western Blot.
Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human Stathmin‑2/STMN2 Monoclonal Antibody (Catalog # MAB6930) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Stathmin‑1 and recombinant human Stathmin‑2 (20 ng/lane) were included. A specific band was detected for Stathmin‑2/STMN2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Stathmin‑2/STMN2 in Human Liver.
Stathmin‑2/STMN2 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Stathmin‑2/STMN2 Monoclonal Antibody (Catalog # MAB6930) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Stathmin-2 (STMN2), also known as superior cervical ganglion 10 protein (SCG10), is a 19 kDa microtubule-destabilizing protein that is preferentially expressed in neurons. It promotes neuronal proliferation and migration as well as neurite outgrowth. Stathmin-2 contains a membrane attachment region (aa 1‑26), a regulatory domain (aa 39-96), and an overlapping coiled-coil domain (aa 75-179). It is subject to phosphorylation of multiple serines within the regulatory domain. Within aa 26‑179, human Stathmin-2 shares 100% and 99% aa sequence identity with mouse and rat Stathmin-2, respectively.
Entrez Gene IDs:
11075 (Human); 20257 (Mouse); 84510 (Rat)
FLJ34868; FLJ50995; neuron-specific growth-associated protein; Protein SCG10; SCG10; SCG10neuronal growth-associated protein (silencer element); SCGN10; SCGN10stathmin-2; Stathmin2; Stathmin-2; stathmin-like 2; STMN2; superior cervical ganglia, neural specific 10; Superior cervical ganglion-10 protein
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