Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 684433
View all Stathmin-2/STMN2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Stathmin-2/STMN2
Tyr26-Gly179
Accession # Q93045

Specificity

Detects human Stathmin-2/STMN2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Stathmin-1, -3, or -4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse Stathmin‑2/STMN2 Antibody

Detection of Human Stathmin-2/STMN2 antibody by Western Blot.

Detection of Human Stathmin‑2/STMN2 by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human Stathmin-2/STMN2 Monoclonal Antibody (Catalog # MAB6930) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Stathmin-1 and recombinant human Stathmin-2 (20 ng/lane) were included. A specific band was detected for Stathmin-2/STMN2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human, Mouse, and Rat Stathmin‑2/STMN2 by Western Blot.

Western blot shows lysates of Neuro‑2A mouse neuroblastoma cell line, SH‑SY5Y human neuroblastoma cell line, Mouse Brain, and Rat Brain. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse Stathmin‑2/STMN2 Monoclonal Antibody (Catalog # MAB6930) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Stathmin‑2/STMN2 at approximately 21-23 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Stathmin-2/STMN2 antibody in Human Liver by Immunohistochemistry (IHC-P).

Stathmin‑2/STMN2 in Human Liver.

Stathmin-2/STMN2 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Stathmin-2/STMN2 Monoclonal Antibody (Catalog # MAB6930) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Mouse Stathmin‑2/STMN2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Western Blot

0.05 - 0.2 µg/mL
Sample: Neuro‑2A mouse neuroblastoma cell line, SH‑SY5Y human neuroblastoma cell line, Mouse Brain, and Rat Brain

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Stathmin-2/STMN2

Stathmin-2 (STMN2), also known as superior cervical ganglion 10 protein (SCG10), is a 19 kDa microtubule-destabilizing protein that is preferentially expressed in neurons. It promotes neuronal proliferation and migration as well as neurite outgrowth. Stathmin-2 contains a membrane attachment region
(aa 1‑26), a regulatory domain (aa 39-96), and an overlapping coiled-coil domain (aa 75-179). It is subject to phosphorylation of multiple serines within the regulatory domain. Within aa 26‑179, human Stathmin-2 shares 100% and 99% aa sequence identity with mouse and rat Stathmin-2, respectively.

Alternate Names

SCG10, SCGN10, Stathmin2, STMN2

Entrez Gene IDs

11075 (Human); 20257 (Mouse); 84510 (Rat)

Gene Symbol

STMN2

UniProt

Q93045

Additional Stathmin-2/STMN2 Products

Product Documents for Human/Mouse Stathmin‑2/STMN2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Stathmin‑2/STMN2 Antibody

For research use only

Related Research Areas

Microtubules

Citations for Human/Mouse Stathmin‑2/STMN2 Antibody

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Protocols

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