Detection of Human and Mouse TORC2 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U2OS human osteosarcoma cell line, MDA‑MB‑453 human breast cancer cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 0.01 µg/mL of Mouse Anti-Human/Mouse TORC2 Monoclonal Antibody (Catalog # MAB6338) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TORC2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TORC2, also known as CREB-regulated transcription co-activator 1 (crtc2), is an approximately 75 kDa protein that functions as a co‑activator for CREB1 in promoting transcription through both consensus and variant cAMP response element (CRE) sites. TORC2 activity is important in regulating the expression of genes involved in cellular energy metabolism. Its O-glycosylation triggers TORC2-dependent induction of hepatic gluconeogenic enzymes. It mediates hypothalamic glucose sensing and also the sensing of endoplasmic reticulum stress. In addition, TORC2 contributes to the transcription of HTLV-1 genes and the activation of latent EBV infections. Within aa 453‑613, human TORC2 shares 84% aa sequence identity with mouse TORC2.
Transducer of Regulated CREB protein 1
Entrez Gene IDs:
200186 (Human); 74343 (Mouse); 310615 (Rat)
CREB regulated transcription coactivator 2; CREB-regulated transcription coactivator 2; CRTC2; TORC2; TORC-2; TORC2transducer of regulated cAMP response element-binding protein (CREB) 2; Transducer of CREB protein 2; Transducer of regulated cAMP response element-binding protein 2
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