Detection of Human and Mouse ZBTB7A/Pokemon by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse ZBTB7A/Pokemon Monoclonal Antibody (Catalog # MAB3496) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ZBTB7A/Pokemon at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
ZBTB7A (zinc finger and BTB domain-containing 7A), also known as Pokemon, FBI-1 in human, LRF in mouse, and OCZF in rat, is a widely expressed nuclear protein that is overexpressed in many human cancers. It is a transcriptional repressor of ARF, c-fos, c-myc, Rb and others, including many extracellular matrix genes. These targets play important roles, both in normal differentiation and oncogenesis. Human ZBTB7A/Pokemon is a 584 amino acid (aa) protein with a BTB-POZ domain (aa 24‑131) and four zinc finger repeats (aa 382‑484). Human ZBTB7A/Pokemon shares 98% aa identity with mouse and rat within aa 349‑476.
Zinc Finger and BTB Domain-Containing Protein 7A
Entrez Gene IDs:
51341 (Human); 16969 (Mouse); 117107 (Rat)
DKFZp547O146; Factor binding IST protein 1; Factor that binds to inducer of short transcripts protein 1; FBI-1; FBI-1POK erythroid myeloid ontogenic factor; FBI1TTF-I-interacting peptide 21; HIV-1 inducer of short transcripts binding protein; Leukemia/lymphoma-related factor; LRF; LRFHIV-1 1st-binding protein 1; lymphoma related factor; MGC99631; pokemon; TIP21; ZBTB7zinc finger and BTB domain containing 7; zinc finger and BTB domain containing 7A; zinc finger and BTB domain containing 7A, HIV-1 inducer of short transcriptsbinding protein; zinc finger and BTB domain-containing protein 7A; Zinc finger protein 857A; ZNF857APOZ and Krueppel erythroid myeloid ontogenic factor