Human MT2A Antibody

Catalog # Availability Size / Price Qty
MAB10176-100
MAB10176-SP
Human MT2A Antibody in Immunohistochemistry
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Human MT2A Antibody Summary

Species Reactivity
Human
Specificity
Detects human MT2A in diect ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 1006811
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Synthetic peptide containing human MT2A
Accession # P02795
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry MT2A in Human Liver. View Larger

MT2A in Human Liver. MT2A was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human MT2A Monoclonal Antibody (Catalog # MAB10176) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MT2A

Metallothionein-2 (MT2A) is a protein that is transcriptionally regulated by heavy metals and glucocorticoids. This Metallothionein (MT) binds zinc. MTs sequester and/or dispense zinc during stress and inflammation to protect cells against reactive oxygen species.

Long Name
Metallothionein 2A
Entrez Gene IDs
4502 (Human); 17750 (Mouse)
Alternate Names
MT2A; MT-II

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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