Human Musashi-1 Antibody MAB2628: R&D Systems

Human Musashi-1 Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Musashi-1 in Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 282613
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Musashi-1
    aa 1-362
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Musashi-1 by Western Blot.
Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human Musashi‑1 Monoclonal Antibody (Catalog # MAB2628) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Musashi-1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Musashi-1
Musashi-1 is an RNA-binding protein that is expressed in the developing central nervous system. Expression is highest in proliferating multipotent neural precursor cells and decreases during neuronal differentiation (1, 2).
  • References:
    1. Kaneko, Y. et al. (2000) Dev. Neurosci. 22:139.
    2. Kanemura, Y. et al. (2002) Meth. Mol. Biol. 198:273.
  • Entrez Gene IDs:
    4440 (Human)
  • Alternate Names:
    MSI1; Musashi (Drosophila) homolog 1; musashi homolog 1 (Drosophila); Musashi1; Musashi-1; RNA-binding protein Musashi homolog 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Integrin ?7 Is a Functional Marker and Potential Therapeutic Target in Glioblastoma
    Authors: TL Haas, MR Sciuto, L Brunetto, C Valvo, M Signore, ME Fiori, S di Martino, S Giannetti, L Morgante, A Boe, M Patrizii, U Warnken, M Schnölzer, A Ciolfi, C Di Stefano, M Biffoni, L Ricci-Viti, R Pallini, R De Maria
    Cell Stem Cell, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. Pharmacological inhibition of poly(ADP-ribose) polymerase-1 modulates resistance of human glioblastoma stem cells to temozolomide.
    Authors: Tentori, Lucio, Ricci-Vitiani, Lucia, Muzi, Alessia, Ciccarone, Fabio, Pelacchi, Federica, Calabrese, Roberta, Runci, Daniele, Pallini, Roberto, Caiafa, Paola, Graziani, Grazia
    BMC Cancer, 2014;14(0):151.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  3. Isolation of cancer stem cells from three human glioblastoma cell lines: characterization of two selected clones.
    Authors: Iacopino F, Angelucci C, Piacentini R, Biamonte F, Mangiola A, Maira G, Grassi C, Sica G
    PLoS ONE, 2014;9(8):e105166.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. Identification of cells with colony-forming activity, self-renewal capacity, and multipotency in ovarian endometriosis.
    Authors: Chan RW, Ng EH, Yeung WS
    Am. J. Pathol., 2011;178(6):2832-44.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Mouse IgG2B Antibody

Flow MAB0043  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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