Detection of Musashi‑2 in Rat Cortical Stem Cells by Flow Cytometry.
Rat cortical stem cells were stained with Mouse Anti-Human Musashi‑2 Monoclonal Antibody (Catalog # MAB3255, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Musashi‑2 in K562 Human Cell Line.
Musashi‑2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human Musashi‑2 Monoclonal Antibody (Catalog # MAB3255) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm (punctate). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Musashi-2 belongs to the evolutionarily conserved Musashi family of RNA
binding proteins which are involved in the translational control of
their target mRNAs. Musashi-2 has two tandem RNA-recognition domains
(RRM-1 and RRM-2) that have the highly conserved RNP
(ribonucleoprotein) motifs. By alternative splicing, at least three
Musashi-2 isoforms exist. In mammalian nervous system, Musashi-1 and –2
are selectively expressed in neural progenitor cells and play important
roles in maintenance of the stem cell fate. Human Musashi-2 shares 95%
amino acid sequence homology with mouse Musashi-2 and 80% amino acid
sequence identity with human Musashi-1. Translocations resulting in the
formation of Musashi-2/HOXA9 fusion protein is associated with
progression of chronic myelogenous leukemia to the accelerated phase
and blast crisis.
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