Human MYH6 Antibody

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Human MYH6 Antibody in Data
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Product Details
Citations (1)
Supplemental Products
Reviews (1)

Human MYH6 Antibody Summary

Species Reactivity
Detects human MYH6. The peptide immunogen sequence represents a region specific to MYH6 and not present in other MYH isoforms.
Monoclonal Mouse IgG1 Clone # 940344
Protein A or G purified from hybridoma culture supernatant
Human MYH6 peptide
Accession # P13533
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
10 µg/mL
See below
5-25 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human MYH6 antibody by Western Blot. View Larger

Detection of Human MYH6 by Western Blot. Western blot shows lysates of human heart (atrium) tissue. PVDF membrane was probed with 10 µg/mL of Mouse Anti-Human MYH6 Monoclonal Antibody (Catalog # MAB8979) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MYH6 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry MYH6 antibody in Human Heart by Immunohistochemistry (IHC-P). View Larger

MYH6 in Human Heart. MYH6 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human MYH6 Monoclonal Antibody (Catalog # MAB8979) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry MYH6 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

MYH6 in BG01V Human Embryonic Stem Cells. MYH6 was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Mouse Anti-Human MYH6 Monoclonal Antibody (Catalog # MAB8979) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MYH6

The MYH6 gene encodes the alpha chain of cardiac myosin, a muscle motor protein with two regions that bind actin at aa 657-679 and 759-773. Defects in this gene cause atrial septal defect 3, hypertrophic cardiac myopathy and sick sinus syndrome. MYH6 participates in embryonic and adult heart development and muscle contraction.  In adult heart, the beta chain isoform predominates in the ventricular tissue, while both isoforms are found in variable proportions in atrial tissue.  Force development is much faster in atrial tissue and ATP consumption is much higher.

Long Name
Myosin Heavy Chain 6, Cardiac Muscle, Alpha
Entrez Gene IDs
4624 (Human); 17888 (Mouse); 29556 (Rat)
Alternate Names
alpha-MHC; ASD3; cardiomyopathy; CMD1EE; CMH14; Hypertrophic 1; MYH6; MYHC; MYHCA; MyHC-Alpha; Myosin Heavy Chain 6; Myosin Heavy Chain, Cardiac Muscle Alpha Isoform; Myosin, Heavy Chain 6, Cardiac Muscle, Alpha; Myosin, Heavy Polypeptide 6, Cardiac Muscle, Alpha (Cardiomyopathy, Hypertrophic 1); Myosin-6; SSS3

Product Datasheets

Citation for Human MYH6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. MAP4K4 Inhibition Promotes Survival of Human Stem Cell-Derived Cardiomyocytes and Reduces Infarct Size In�Vivo
    Authors: LR Fiedler, K Chapman, M Xie, E Maifoshie, M Jenkins, PA Golforoush, M Bellahcene, M Noseda, D Faust, A Jarvis, G Newton, MA Paiva, M Harada, DJ Stuckey, W Song, J Habib, P Narasimham, R Aqil, D Sanmugalin, R Yan, L Pavanello, M Sano, SC Wang, RD Sampson, S Kanayagana, GE Taffet, LH Michael, ML Entman, TH Tan, SE Harding, CMR Low, C Tralau-Ste, T Perrior, MD Schneider
    Cell Stem Cell, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC


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Human MYH6 Antibody
By Anonymous on 02/26/2019
Application: ICC/IF Sample Tested: hiPSC-derived cardiomyocytes Species: Human

This antibody was diluted 1:50 (10ug/ml) and used for IF staining of human iPSC - derived cardiomyocytes (15 days after initiation of differentiation).