Human MYH6 Antibody (940344)

R&D Systems | Catalog # MAB8979

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 940344
View all MYH6 Primary Antibodies »

Product Specifications

Immunogen

Human MYH6 peptide
Accession # P13533

Specificity

Detects human MYH6. The peptide immunogen sequence represents a region specific to MYH6 and not present in other MYH isoforms.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human MYH6 Antibody (940344)

MYH6 antibody in Human Heart by Immunohistochemistry (IHC-P).

MYH6 in Human Heart.

MYH6 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human MYH6 Monoclonal Antibody (Catalog # MAB8979) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
MYH6 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

MYH6 in BG01V Human Embryonic Stem Cells.

MYH6 was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Mouse Anti-Human MYH6 Monoclonal Antibody (Catalog # MAB8979) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Detection of Human MYH6 by Western Blot.

Western blot shows lysates of human heart atrium. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MYH6 Monoclonal Antibody (Catalog # MAB8979) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MYH6 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of aca2xvxrfugv1hkppchb.jpg by Immunohistochemistry

Detection of aca2xvxrfugv1hkppchb.jpg by Immunohistochemistry

ACTN2 accumulation and defective F-ACTIN polymerization during the nascent myofibril stage in UNC45B−/−-CMs(A and B) Representative IF images of MYH6, MYH10, and TTN-C in non-replated WT-CMs and UNC45B−/−-CMs harvested at day 7 or day 10. Cells were stained for (A) MYH6 and MYH10 and (B) TTN-C and MYH6. To improve visualization of MYH6 signal in the UNC45B−/− cells, additional sets of MYH6 images with a longer exposure time are shown. Scale bars: 10 μm.(C) Representative IF images of non-replated WT-CMs and UNC45B−/−-CMs harvested at day 7 or day 10 and stained for TTN-N and ACTN2. Scale bars: 10 μm.(D) Quantification results for ACTN2 signal intensity in WT-CMs and UNC45B−/−-CMs at day 7, showing ACTN2 accumulation (n = 150). Error bars represent SD. Statistical significance is indicated: ∗∗∗p < 0.001.(E and F) Left panel: representative IF images of WT-CMs and UNC45B−/−-CMs harvested at (E) day 7 and (F) day 10 and stained for ACTN2 and F-ACTIN. Scale bars: 10 μm. Results represent data from at least three independent experiments.See also Figure S3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37295424), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of cqbumwuxr5qxaj4pkse2.jpg by Immunocytochemistry/ Immunofluorescence

Detection of cqbumwuxr5qxaj4pkse2.jpg by Immunocytochemistry/ Immunofluorescence

Effect of ectopic UNC45B expression in WT-CMs and UNC45B−/−:-CMs(A) Western blots of the protein expression levels of UNC45B in WT-CMs, WTUNC45B-OE-CMs, and UNC45B−/−:OE-CMs. Exogenous and endogenous UNC45B are indicated.(B) Representative TEM images of WT-CMs and UNC45B−/−:OE-CMs. Scale bars are indicated.(C) Representative IF images of replated WT-CMs and WTUNC45B-OE-CMs harvested at day 30 and stained for the ACTN2 and TNNT2 (upper panel) or for MYH6 and F-ACTIN (bottom panel). Scale bars: 10 μm. Results represent data from at least three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37295424), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human MYH6 Antibody (940344)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart

Western Blot

10 µg/mL
Sample: Human heart (atrium) tissue

Reviewed Applications

Read 2 reviews rated 4.5 using MAB8979 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MYH6

The MYH6 gene encodes the alpha chain of cardiac myosin, a muscle motor protein with two regions that bind actin at aa 657-679 and 759-773. Defects in this gene cause atrial septal defect 3, hypertrophic cardiac myopathy and sick sinus syndrome. MYH6 participates in embryonic and adult heart development and muscle contraction.  In adult heart, the beta chain isoform predominates in the ventricular tissue, while both isoforms are found in variable proportions in atrial tissue.  Force development is much faster in atrial tissue and ATP consumption is much higher.

Long Name

Myosin Heavy Chain 6, Cardiac Muscle, Alpha

Alternate Names

alpha-MHC, ASD3, CMD1EE, CMH14, MyHC-Alpha, MYHCA, Myosin-6, SSS3

Entrez Gene IDs

4624 (Human); 17888 (Mouse); 29556 (Rat)

Gene Symbol

MYH6

UniProt

P13533

Additional MYH6 Products

Product Documents for Human MYH6 Antibody (940344)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MYH6 Antibody (940344)

For research use only

Related Research Areas

Cardiac Stem Cell Markers

Citations for Human MYH6 Antibody (940344)

Customer Reviews for Human MYH6 Antibody (940344) (2)

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2 Customer Ratings
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  • Human MYH6 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Pancreas carcinoma
    Species: Human
    Verified Customer | Posted 11/18/2021
    Human MYH6 Antibody (940344) MAB8979
  • Human MYH6 Antibody
    Name: Yongshun Lin
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: hiPSC-derived cardiomyocytes
    Species: Human
    Verified Customer | Posted 02/26/2019
    This antibody was diluted 1:50 (10ug/ml) and used for IF staining of human iPSC - derived cardiomyocytes (15 days after initiation of differentiation).
    Human MYH6 Antibody (940344) MAB8979

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