Human N-Cadherin Antibody (691721R)

R&D Systems | Catalog # MAB13883

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 691721R
View all N-Cadherin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human N-Cadherin
Asp160-Ala724
Accession # P19022.4

Specificity

Detects human N- Cadherin in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human N-Cadherin Antibody (691721R)

Human N-Cadherin Antibody in ELISA Standard Curve.

Human N‑Cadherin ELISA Standard Curve.

Recombinant Human N-Cadherin protein was serially diluted 2-fold and captured by Mouse Anti-Human N-Cadherin Monoclonal Antibody (Catalog # MAB13883) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human/Mouse/Rat N-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6426) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human N-Cadherin Antibody (691721R)

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Sheep Anti-Human/Mouse/Rat N‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6426).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human N-Cadherin DuoSet ELISA Kit (Catalog # DY1388-05) for convenient development of a sandwich ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: N-Cadherin

Neuronal Cadherin (N-Cadherin or NCAD), also known as Cadherin-2 (CDH2), is a 130 kDa type I membrane protein belonging to the Cadherin superfamily of calcium-dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1, 2). Human N‑Cadherin is synthesized with a 25 amino acid (aa) signal peptide and a 134 aa N‑terminal propeptide. The mature cell surface‑expressed protein consists of a 565 amino acid (aa) extracellular domain (ECD) that contains five Cadherin repeats, a 21 aa transmembrane segment, and a 161 aa cytoplasmic domain (3). Within the ECD, human N-Cadherin shares 98% aa sequence identity with mouse and rat N-Cadherin. In the nervous system, N-Cadherin mediates adhesion between the opposing faces of developing neuronal synapses and between Schwann cells and neuronal axons (4, 5). It interacts in cis or in trans homophilically and with the GluR2 subunit of neuronal AMPA receptors (1, 6). During synaptic maturation, its expression is lost from inhibitory terminals but maintained at excitatory terminals (5). ADAM10-mediated shedding of the N-Cadherin ECD alters cell-cell adhesion, synaptic development, and AMPA receptor activity (7, 8). N-Cadherin can also be cleaved at multiple additional sites within the intracellular or extracellular domains by Calpain, gamma ‑Secretase, and several MMPs (9-13). Cleavage of N‑Cadherin in atherosclerotic plaques contributes alternatively to vascular smooth muscle cell proliferation (MMP-9 and -12) or apoptosis (MMP‑7 (12, 13). Aberrant cell surface expression of the pro and mature forms of N-Cadherin in cancer results in increased tumor progression and invasiveness (14, 15). N‑Cadherin also mediates the adhesion between hematopoeitic progenitor cells and mesenchymal stromal cells of the bone marrow (16).

References

  1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  2. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
  3. Reid, R.A. and J.J. Hemperly (1990) Nucleic Acids Res. 18:5896.
  4. Wanner, I.B. and P.M. Wood (2002) J. Neurosci. 22:4066.
  5. Benson, D.L. and H. Tanaka (1998) J. Neurosci. 18:6892.
  6. Saglietti, L. et al. (2007) Neuron 54:461.
  7. Reiss, K. et al. (2005) EMBO J. 24:742.
  8. Malinverno, M. et al. (2010) J. Neurosci. 30:16343.
  9. Jang, Y.-N. et al. (2009) J. Neurosci. 29:5974.
  10. Uemura, K. et al. (2006) Neurosci. Lett. 402:278.
  11. Hartland, S.N. et al. (2009) Liver Int. 29:966.
  12. Williams, H. et al. (2010) Cardiovasc. Res. 87:137.
  13. Dwivedi, A. et al. (2009) Cardiovasc. Res. 81:178.
  14. Maret, D. et al. (2010) Neoplasia 12:1066.
  15. Tanaka, H. et al. (2010) Nat. Med. 16:1414.
  16. Wein, F. et al. (2010) Stem Cell Res. 4:129.

Long Name

Neural Cadherin

Alternate Names

Cadherin-2, CD325, CDH2, NCadherin

Entrez Gene IDs

1000 (Human); 12558 (Mouse); 83501 (Rat)

Gene Symbol

CDH2

UniProt

P19022.4

Additional N-Cadherin Products

Product Documents for Human N-Cadherin Antibody (691721R)

Certificate of Analysis

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Product Specific Notices for Human N-Cadherin Antibody (691721R)

For research use only

Citations for Human N-Cadherin Antibody (691721R)

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