Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 817227
View all NAALADase-like 2/NAALADL2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NAALADase‑like 2/NAALADL2
Ser152-Asn795
Accession # Q58DX5

Specificity

Detects human NAALADase‑like 2/NAALADL2 in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human NAALADase‑like 2/NAALADL2 Antibody

NAALADase-like 2/NAALADL2 antibody in Human Kidney by Immunohistochemistry (IHC-P).

NAALADase‑like 2/NAALADL2 in Human Kidney.

NAALADase-like 2/NAALADL2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human NAALADase-like 2/ NAALADL2 Monoclonal Antibody (Catalog # MAB46651) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human NAALADase‑like 2/NAALADL2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Reviewed Applications

Read 1 review rated 5 using MAB46651 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: NAALADase-like 2/NAALADL2

NAALADL2 (N-acetylated alpha -linked acidic dipeptidase like 2) is a member of the peptidase M28 family of enzymes. It is presumably a type II transmembrane (TM) protein that may have O-glycosyl hydrolase activity. Human NAALADL2 is 795 amino acids (aa) in length. It is believed to contain a cytoplasmic and TM segment at the N-terminus, followed by a peptidase domain (aa 444‑596) and a TfR-like dimerization region (aa 688‑777). There are multiple splice forms. One shows a 4 aa substitution for aa 292‑795, a second shows an alternate start site at Met18 with a 24 aa substitution for aa 314‑795, and a third contains an alternate start site at Met283, accompanied by a deletion of aa 363‑411 and 633‑795. Over aa 152‑795, human NAALADL2 shares 87% and 82% aa sequence identity with mouse and canine NAALADL2, respectively.

Long Name

N-Acetylated Alpha-Linked Acidic Dipeptidase-like 2

Alternate Names

NAALADase like 2, NAALADL2

Entrez Gene IDs

254827 (Human); 635702 (Mouse); 499583 (Rat)

Gene Symbol

NAALADL2

UniProt

Q58DX5

Additional NAALADase-like 2/NAALADL2 Products

Product Documents for Human NAALADase‑like 2/NAALADL2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human NAALADase‑like 2/NAALADL2 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Customer Reviews for Human NAALADase‑like 2/NAALADL2 Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human NAALADase‑like 2/NAALADL2 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human NAALADase-like 2/NAALADL2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Kidney tissue
    Species: Human
    Verified Customer | Posted 12/26/2021
    Human NAALADase‑like 2/NAALADL2 Antibody MAB46651

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies