Nectin‑4 in Human Placenta. Nectin‑4 was detected in immersion fixed paraffin-embedded sections of human placenta using 10 µg/mL Goat Anti-Human Nectin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2659) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin
family of Ig superfamily proteins. It is both a homophilic and
heterophilic (with Nectin-1) cell adhesion molecule that is expressed
in the embryo and in breast carcinoma. A soluble form of Nectin-4 is
generated from the membrane protein via the action of TACE/ADAM-17. The
extracellular domain of human Nectin-4 shares 90% and 92% amino acid
sequence homology with the corresponding regions of mouse and rat
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