Human Nectin-4 Antibody

(9 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Nectin-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross‑reactivity with recombinant mouse Nectin-4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Nectin-4
    Gly27-Val351
    Accession # Q96NY8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Nectin‑4 (Catalog # 2659-N4)
  • Flow Cytometry
    0.25 µg/106 cells
    MCF‑7 human breast cancer cell line
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Nectin‑4 in Human Placenta. Nectin‑4 was detected in immersion fixed paraffin-embedded sections of human placenta using 10 µg/mL Goat Anti-Human Nectin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2659) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nectin-4
Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the embryo and in breast carcinoma. A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of human Nectin-4 shares 90% and 92% amino acid sequence homology with the corresponding regions of mouse and rat Nectin-4, respectively.
  • Long Name:
    Poliovirus Receptor Related 4
  • Entrez Gene IDs:
    81607 (Human); 71740 (Mouse)
  • Alternate Names:
    EDSS1; Ig superfamily receptor LNIR; LNIR; LNIRnectin 4; Nectin4; Nectin-4; poliovirus receptor-related 4; poliovirus receptor-related protein 4; PRR4; PRR4DKFZp686K05193; PVRL4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
Filter your results:

Species
Applications
Sample Type
  1. Expression of canine distemper virus receptor nectin-4 in the central nervous system of dogs
    Authors: W Pratakpiri, AP Ping Teh, A Radtanakat, N Pirarat, N Thi Lan, M Takeda, S Techangams, R Yamaguchi
    Sci Rep, 2017;7(1):349.
    Species: Canine
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  2. A Novel Nectin-mediated Cell Adhesion Apparatus That Is Implicated in Prolactin Receptor Signaling for Mammary Gland Development
    Authors: M Kitayama, K Mizutani, M Maruoka, K Mandai, S Sakakibara, Y Ueda, T Komori, Y Shimono, Y Takai
    J. Biol. Chem, 2016;291(11):5817-31.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  3. Oncolytic measles virus expressing the sodium iodide symporter to treat drug-resistant ovarian cancer.
    Authors: Galanis E, Atherton P, Maurer M, Knutson K, Dowdy S, Cliby W, Haluska P, Long H, Oberg A, Aderca I, Block M, Bakkum-Gamez J, Federspiel M, Russell S, Kalli K, Keeney G, Peng K, Hartmann L
    Cancer Res, 2015;75(1):22-30.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  4. SLAM- and Nectin-4-Independent Noncytolytic Spread of Canine Distemper Virus in Astrocytes.
    Authors: Alves L, Khosravi M, Avila M, Ader-Ebert N, Bringolf F, Zurbriggen A, Vandevelde M, Plattet P
    J Virol, 2015;89(10):5724-33.
    Species: Canine
    Sample Type: Whole Tissue
    Application: IHC
  5. Nectin-4 expression contributes to tumor proliferation, angiogenesis and patient prognosis in human pancreatic cancer.
    Authors: Nishiwada S, Sho M, Yasuda S, Shimada K, Yamato I, Akahori T, Kinoshita S, Nagai M, Konishi N, Nakajima Y
    J Exp Clin Cancer Res, 2015;34(0):30.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - paraffin
  6. Nectin-4 mutations causing ectodermal dysplasia with syndactyly perturb the rac1 pathway and the kinetics of adherens junction formation.
    Authors: Fortugno P, Josselin E, Tsiakas K, Agolini E, Cestra G, Teson M, Santer R, Castiglia D, Novelli G, Dallapiccola B, Kurth I, Lopez M, Zambruno G, Brancati F
    J Invest Dermatol, 2014;134(8):2146-53.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  7. Lethal canine distemper virus outbreak in cynomolgus monkeys in Japan in 2008.
    J Virol, 2013;87(2):1105-14.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. A role for PVRL4-driven cell-cell interactions in tumorigenesis.
    Authors: Pavlova N, Pallasch C, Elia A, Braun C, Westbrook T, Hemann M, Elledge S
    Elife, 2013;2(0):e00358.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  9. Tumor cell marker PVRL4 (nectin 4) is an epithelial cell receptor for measles virus.
    Authors: Noyce RS, Bondre DG, Ha MN
    PLoS Pathog., 2011;7(8):e1002240.
    Species: Hamster
    Sample Type: Whole Cells
    Application: Neut
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