Human Nectin-4 Antibody

Catalog # Availability Size / Price Qty
AF2659
AF2659-SP
Nectin‑4 in Human Placenta.
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Product Details
Citations (11)
FAQs
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Human Nectin-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Nectin-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross‑reactivity with recombinant mouse Nectin-4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Nectin-4
Gly27-Val351
Accession # Q96NY8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Nectin‑4 (Catalog # 2659-N4)
Flow Cytometry
0.25 µg/106 cells
MCF‑7 human breast cancer cell line
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry Nectin‑4 in Human Placenta. View Larger

Nectin‑4 in Human Placenta. Nectin-4 was detected in immersion fixed paraffin-embedded sections of human placenta using 10 µg/mL Goat Anti-Human Nectin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2659) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Flow Cytometry Detection of Nectin-4 in MCF-7 Human Cell Line by Flow Cytometry. View Larger

Detection of Nectin-4 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast adenocarcinoma cell line was stained with Goat Anti-Human Nectin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2659, filled histogram) or control antibody (AB-108-C, open histogram), followed by PE-conjugated Anti-Goat IgG Secondary Antibody (F0107).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nectin-4

Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the embryo and in breast carcinoma. A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of human Nectin-4 shares 90% and 92% amino acid sequence homology with the corresponding regions of mouse and rat Nectin-4, respectively.

Long Name
Poliovirus Receptor Related 4
Entrez Gene IDs
81607 (Human); 71740 (Mouse); 498281 (Rat); 102127936 (Cynomolgus Monkey)
Alternate Names
EDSS1; Ig superfamily receptor LNIR; LNIR; LNIRnectin 4; Nectin4; Nectin-4; poliovirus receptor-related 4; poliovirus receptor-related protein 4; PRR4; PRR4DKFZp686K05193; PVRL4

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Citations for Human Nectin-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Antitumor activity of an oncolytic measles virus against canine urinary bladder transitional cell carcinoma cells
    Authors: K Iizuka, K Shoji, T Fujiyuki, K Moritoh, K Tamura, A Yoshida, H Sato, M Yoneda, K Asano, C Kai
    Research in Veterinary Science, 2020;0(0):.
    Species: Canine
    Sample Types: Whole Tissue
    Applications: IHC
  2. Over-expression of Nectin-4 promotes progression of esophageal cancer and correlates with poor prognosis of the patients
    Authors: H Deng, H Shi, L Chen, Y Zhou, J Jiang
    Cancer Cell Int., 2019;19(0):106.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Expression of canine distemper virus receptor nectin-4 in the central nervous system of dogs
    Authors: W Pratakpiri, AP Ping Teh, A Radtanakat, N Pirarat, N Thi Lan, M Takeda, S Techangams, R Yamaguchi
    Sci Rep, 2017;7(1):349.
    Species: Canine
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. A Novel Nectin-mediated Cell Adhesion Apparatus That Is Implicated in Prolactin Receptor Signaling for Mammary Gland Development
    Authors: M Kitayama, K Mizutani, M Maruoka, K Mandai, S Sakakibara, Y Ueda, T Komori, Y Shimono, Y Takai
    J. Biol. Chem, 2016;291(11):5817-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  5. SLAM- and Nectin-4-Independent Noncytolytic Spread of Canine Distemper Virus in Astrocytes.
    Authors: Alves L, Khosravi M, Avila M, Ader-Ebert N, Bringolf F, Zurbriggen A, Vandevelde M, Plattet P
    J Virol, 2015;89(10):5724-33.
    Species: Canine
    Sample Types: Whole Tissue
    Applications: IHC
  6. Nectin-4 expression contributes to tumor proliferation, angiogenesis and patient prognosis in human pancreatic cancer.
    Authors: Nishiwada S, Sho M, Yasuda S, Shimada K, Yamato I, Akahori T, Kinoshita S, Nagai M, Konishi N, Nakajima Y
    J Exp Clin Cancer Res, 2015;34(0):30.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Oncolytic measles virus expressing the sodium iodide symporter to treat drug-resistant ovarian cancer.
    Authors: Galanis E, Atherton P, Maurer M, Knutson K, Dowdy S, Cliby W, Haluska P, Long H, Oberg A, Aderca I, Block M, Bakkum-Gamez J, Federspiel M, Russell S, Kalli K, Keeney G, Peng K, Hartmann L
    Cancer Res, 2015;75(1):22-30.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. Nectin-4 mutations causing ectodermal dysplasia with syndactyly perturb the rac1 pathway and the kinetics of adherens junction formation.
    Authors: Fortugno P, Josselin E, Tsiakas K, Agolini E, Cestra G, Teson M, Santer R, Castiglia D, Novelli G, Dallapiccola B, Kurth I, Lopez M, Zambruno G, Brancati F
    J Invest Dermatol, 2014;134(8):2146-53.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Lethal canine distemper virus outbreak in cynomolgus monkeys in Japan in 2008.
    J Virol, 2013;87(2):1105-14.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. A role for PVRL4-driven cell-cell interactions in tumorigenesis.
    Authors: Pavlova N, Pallasch C, Elia A, Braun C, Westbrook T, Hemann M, Elledge S
    Elife, 2013;2(0):e00358.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  11. Tumor cell marker PVRL4 (nectin 4) is an epithelial cell receptor for measles virus.
    Authors: Noyce RS, Bondre DG, Ha MN
    PLoS Pathog., 2011;7(8):e1002240.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Neutralization

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