Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 466501
View all Neuromedin BR/NMBR Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Neuromedin B R/NMBR
Accession # NP_002502

Specificity

Detects human Neuromedin B R/NMBR in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Neuromedin BR/NMBR Antibody

Neuromedin B R/NMBR antibody in Human Colon Cancer Tissue by Immunohistochemistry (IHC-P).

Neuromedin B R/NMBR in Human Colon Cancer Tissue.

Neuromedin B R/NMBR was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Human Neuromedin B R/NMBR Monoclonal Antibody (Catalog # MAB47281) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Neuromedin BR/NMBR Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue

Reviewed Applications

Read 1 review rated 5 using MAB47281 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neuromedin BR/NMBR

Neuromedin B Receptor (NMBR) is an 80 kDa, 390 amino acid (aa) G-protein coupled 7-transmembrane glycoprotein receptor for bombesin-like peptides, binding Neuromedin B with high affinity and GRP (gastrin releasing peptide) with lower affinity.  Neuromedin B R expression in the olfactory and central thalamic regions of the brain plays a role in thermoregulation. It has also been shown to be mitogenic in colonic epithelium. Expression in the pituitary gland is important for regulation of the pituitary-thyroid axis. Within extracellular domains, human Neuromedin B R shares 86% and 82% aa identity with mouse and rat Neuromedin B R, respectively.

Long Name

Neuromedin B Receptor

Alternate Names

Neuromedin B R, NMBR

Entrez Gene IDs

4829 (Human); 18101 (Mouse); 25264 (Rat)

Gene Symbol

NMBR

UniProt

NP_002502

Additional Neuromedin BR/NMBR Products

Product Documents for Human Neuromedin BR/NMBR Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Neuromedin BR/NMBR Antibody

For research use only

Customer Reviews for Human Neuromedin BR/NMBR Antibody (1)

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  • Human Neuromedin BR/NMBR Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Colon tissue
    Species: Human
    Verified Customer | Posted 12/28/2021
    Human Neuromedin BR/NMBR Antibody MAB47281

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Protocols

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