Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1075611
View all Neurotensin Primary Antibodies »

Product Specifications

Immunogen

Neurotensin containing synthetic peptide
Accession # P30990

Specificity

Detects a synthetic peptide around aa 135 in Direct ELISA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Neurotensin Antibody

Detection of Neurotensin in Human Small Bowel.

Neurotensin was detected in immersion fixed paraffin-embedded sections of human small bowel using Mouse Anti-Human Neurotensin Monoclonal Antibody (Catalog # MAB11548) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neuroendocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Neurotensin Antibody

Application
Recommended Usage

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human small bowel

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neurotensin

Neurotensin is a neuropeptide neurotransmitter expressed in both the central and peripheral nervous systems.  It acts as a neurotransmitter in the central nervous system and as a hormone in the periphery.  This neuropeptide has many roles in the body such as:  temperature regulation, feeding, analgesia, ethanol sensitivity, psychosis, substance use, and pain. Neurotensin may also play an endocrine or paracrine role in the regulation of fat metabolism.  It is expressed in neuronal synaptic vesicles in the CNS and in the periphery is expressed in the GI tract and in neuroendocrine cells.

References

  1. Torruella-Suárez ML, McElligott ZA. Neurotensin in reward processes. Neuropharmacology. 2020 May 1;167:108005. doi: 10.1016/j.neuropharm.2020.108005. Epub 2020 Feb 11. PMID: 32057800; PMCID: PMC7238864. 
  2. Mazella J, Béraud-Dufour S, Devader C, Massa F, Coppola T. Neurotensin and its receptors in the control of glucose homeostasis. Front Endocrinol (Lausanne). 2012 Nov 26;3:143. doi: 10.3389/fendo.2012.00143. PMID: 23230428; PMCID: PMC3515879.

Long Name

Neurotensin/neuromedin N [Cleaved into: Large neuromedin N

Alternate Names

NTS

Entrez Gene IDs

4922 (Human); 280881 (Bovine)

Gene Symbol

NTS

UniProt

P30990 (Human)

Additional Neurotensin Products

Product Documents for Human Neurotensin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Neurotensin Antibody

For research use only

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Protocols

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