Human NGF R/TNFRSF16 Antibody

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  • Species Reactivity
    Human
  • Specificity
    Detects human NGF R/TNFRSF16 in direct ELISAs. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse NGF R is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NGF R/TNFRSF16
    Lys29-Asn250
    Accession # P08138
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
    • Human NGF R Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Human NGF R/TNFRSF16 Antibody (Catalog # AF367 )
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human/Mouse NGF R/TNFRSF16 Biotinylated Antibody (Catalog # BAF367 )
  • ELISA Standard
     
    Recombinant Human NGF R/TNFRSF16 Fc Chimera Protein (Catalog # 367-NR )
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
ELISA Capture (Matched Antibody Pair)
Human/Mouse/Rat NGF R/TNFRSF16 ELISA Standard Curve. Recombinant Human/Mouse/Rat NGF R/TNFRSF16 (Catalog # 367-NR) was serially diluted 2-fold and captured by Goat Anti-Human/Mouse/Rat NGF R/TNFRSF16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF367) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse NGF R/TNFRSF16 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF367) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NGF R/TNFRSF16

p75 neurotrophin receptor, also named low affinity NGF receptor (NGF R), is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family. NGF R cDNA encodes a 427 amino acid (aa) residue precursor protein with a 28 aa residue signal peptide, a 222 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 155 aa residue intracellular domain. The extracellular region contains four cysteine-rich domains and binds NGF, BDNF, NT-3, and NT-4 approximately equally with low affinity. The cytoplasmic region contains a subtype 2 death domain.

NGF R expression has been shown to occur widely during development and in the adult. Expression has been detected in both neuronal and non-neuronal cells. NGF R was originally reported to function as a positive regulator of TrkA activity. NGF R has also been shown to signal by itself. Depending on its cellular environment, NGF R has now been shown to regulate cell migration, gene expression and to mediate apoptosis. Recombinant NGF R Fc chimera binds NGF with high affinity and is a potent NGF antagonist. Naturally occurring truncated NGF R containing the extracellular domain and lacking the transmembrane or intracellular domain has been detected in vivo in urine, plasma and in amniotic fluid of humans and rats.

  • References:
    1. Barker, P.A. and R.A. Murphy (1992) Molecular and Cellular Biochemistry 110:1.
    2. Bamji, A.X. et al. (1998) J. Cell Biol. 140:911.
    3. Feinstein, E. et al. (1995) Trends Biochem. Sci. 20:342.
  • Long Name:
    Nerve Growth Factor Receptor
  • Entrez Gene IDs:
    4804 (Human); 18053 (Mouse); 117089 (Rat)
  • Alternate Names:
    CD271 antigen; CD271; Gp80-LNGFR; member 16; nerve growth factor receptor; NGF R; NGF receptor; NGFR; p75 ICD; p75 NTR; TNFRSF16; TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16); tumor necrosis factor receptor superfamily member 16
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Direct and bystander killing of sarcomas by novel cytosine deaminase fusion gene.
    Authors: Ramnaraine M, Pan W, Goblirsch M, Lynch C, Lewis V, Orchard P, Mantyh P, Clohisy DR
    Cancer Res., 2003;63(20):6847-54.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 203  
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB , Simple Western HAF109 20  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL003 6
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 7  
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB , ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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