Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 631438
View all NKX6.1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NKX6.1
Met1-Pro120
Accession # P78426

Specificity

Detects human NKX6.1 in direct ELISAs and Western blots.  In direct ELISAs, no cross-reactivity with recombinant human NKX2E, 2.2, 3.1 or recombinant mouse NKX3.1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human NKX6.1 Antibody

NKX6 by Immunocytochemistry (ICC).1 antibody in beta TC-6 Mouse Cell Line by Immunocytochemistry (ICC).

NKX6.1 in beta TC‑6 Mouse Cell Line.

NKX6.1 was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human NKX6.1 Monoclonal Antibody (Catalog # MAB5857) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human NKX6.1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed beta TC‑6 mouse beta cell insulinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NKX6.1

NKX6.1 (NK homeobox factor 6.1; also NKX6A) is a 39-43 kDa member of the homeodomain family of transcription factors. It is expressed in embryonic ventral neural tube where it drives neural progenitors to form motoneurons and V2-type interneurons. It is also expressed in both fetal and neonatal pancreas where it promotes a beta -cell phenotype. NKX6.1 exhibits both transcriptional gene repression (NKX6.2 and Dbx2) and activation (cyclin A2 and B1). When overexpressed, it induces  beta -cell mitosis. Human NKX6.1 is 367 amino acids (aa) in length. It contains a repressor region composed of Pro and Ala (aa 136-173), a DNA binding homeobox (aa 236-295) and a Glu:Asp-rich transactivation domain (aa 305-337). Over aa 1-120, human NKX6.1 shares 96% aa identity with mouse NKX6.1.

Long Name

NK6 Homeobox 1

Alternate Names

NKX6-1, NKX6A

Entrez Gene IDs

4825 (Human); 18096 (Mouse); 65193 (Rat)

Gene Symbol

NKX6-1

UniProt

P78426 (Human)

Additional NKX6.1 Products

Product Documents for Human NKX6.1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NKX6.1 Antibody

For research use only

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