Human NKX6.1 Antibody

Catalog # Availability Size / Price Qty
NKX6.1 in  beta TC‑6 Mouse Cell Line.
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Product Details
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Human NKX6.1 Antibody Summary

Species Reactivity
Detects human NKX6.1 in direct ELISAs and Western blots.  In direct ELISAs, no cross-reactivity with recombinant human NKX2E, 2.2, 3.1 or recombinant mouse NKX3.1 is observed.
Monoclonal Mouse IgG2B Clone # 631438
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human NKX6.1
Accession # P78426
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry NKX6 by Immunocytochemistry (ICC).1 antibody in  beta TC-6 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

NKX6.1 in beta TC‑6 Mouse Cell Line. NKX6.1 was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human NKX6.1 Monoclonal Antibody (Catalog # MAB5857) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKX6.1

NKX6.1 (NK homeobox factor 6.1; also NKX6A) is a 39-43 kDa member of the homeodomain family of transcription factors. It is expressed in embryonic ventral neural tube where it drives neural progenitors to form motoneurons and V2-type interneurons. It is also expressed in both fetal and neonatal pancreas where it promotes a beta -cell phenotype. NKX6.1 exhibits both transcriptional gene repression (NKX6.2 and Dbx2) and activation (cyclin A2 and B1). When overexpressed, it induces  beta -cell mitosis. Human NKX6.1 is 367 amino acids (aa) in length. It contains a repressor region composed of Pro and Ala (aa 136-173), a DNA binding homeobox (aa 236-295) and a Glu:Asp-rich transactivation domain (aa 305-337). Over aa 1-120, human NKX6.1 shares 96% aa identity with mouse NKX6.1.

Long Name
NK6 Homeobox 1
Entrez Gene IDs
4825 (Human); 18096 (Mouse); 65193 (Rat)
Alternate Names
Homeobox protein NK-6 homolog A; NK homeo box, family 6, member A; NK homeobox (Drosophila), family 6, A; NK homeobox, family 6, A; NK6 homeobox 1; NK6 transcription factor homolog A; NK6 transcription factor related, locus 1 (Drosophila); NK6 transcription factor related, locus 1; NKX6.1; NKX6-1; NKX6A; NKX6Ahomeobox protein Nkx-6.1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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