NKX6.1 in beta TC‑6 Mouse Cell Line.
NKX6.1 was detected in immersion fixed beta TC‑6 mouse beta cell insulinoma cell line using Human NKX6.1 Monoclonal Antibody (Catalog # MAB5857) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
NKX6.1 (NK homeobox factor 6.1; also NKX6A) is a 39-43 kDa member of the homeodomain family of transcription factors. It is expressed in embryonic ventral neural tube where it drives neural progenitors to form motoneurons and V2-type interneurons. It is also expressed in both fetal and neonatal pancreas where it promotes a beta -cell phenotype. NKX6.1 exhibits both transcriptional gene repression (NKX6.2 and Dbx2) and activation (cyclin A2 and B1). When overexpressed, it induces beta -cell mitosis. Human NKX6.1 is 367 amino acids (aa) in length. It contains a repressor region composed of Pro and Ala (aa 136-173), a DNA binding homeobox (aa 236-295) and a Glu:Asp-rich transactivation domain (aa 305-337). Over aa 1-120, human NKX6.1 shares 96% aa identity with mouse NKX6.1.
NK6 Homeobox 1
Entrez Gene IDs:
4825 (Human); 18096 (Mouse); 65193 (Rat)
Homeobox protein NK-6 homolog A; NK homeo box, family 6, member A; NK homeobox (Drosophila), family 6, A; NK homeobox, family 6, A; NK6 homeobox 1; NK6 transcription factor homolog A; NK6 transcription factor related, locus 1 (Drosophila); NK6 transcription factor related, locus 1; NKX6.1; NKX6-1; NKX6A; NKX6Ahomeobox protein Nkx-6.1
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